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URL: https://biotech.unl.edu/cryoem
Proper Citation: University of Nebraska Lincoln CryoEM Core Facility (RRID:SCR_025012)
Description: Core facility is dedicated to structural characterization of biological samples and microcrystals using cryo-electron microscopy. CryoEM workflows including, single-particle CryoEM, Cryo-electron tomography , and Micro crystal electron diffraction. Instrumentation includes TFS 200kV Glacios CryoTEM, equipped with zero-loss Selectris Energy Filter, Flacon 4i Direct Electron Detector, and Ceta-D camera, Pelco Easy Glow Discharger, TFS Vitrobot Mark IV, and Leica EM GP2. We also present all the capacities for storing and shipping samples. Facility has Data storage Server and Live processing workstation for our users� convenience, including data processing suites RELION and CryoSPARC available at our campus supercomputer Swan cluster at the Holland Computer Center.
Synonyms: CryoEM Core Facility
Resource Type: service resource, access service resource, core facility
Keywords: ABRF, structural characterization, biological samples, microcrystals, cryo-electron microscopy, single-particle CryoEM, Cryo-electron tomography, Micro crystal electron diffraction,
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