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URL: https://louisville.edu/medicine/cancer-research/cores-and-facilities-1/biophysics
Proper Citation: University of Louisville Biophysical Core Facility (RRID:SCR_026277)
Description: Biophysical Core Facility supports structural biology studies focused on characterization of biophysical properties of biomolecules and their interactions with binding partners or novel drug candidates. Offers range of biophysical techniques for initial screening of hit/lead compound binding as well as rigorous characterization of the thermodynamic and hydrodynamic properties of various biological macromolecules (most commonly proteins and nucleic acids), macromolecular association reactions, and ligand-macromolecule interactions. Biophysical techniques include differential scanning fluorimetry, microscale thermophoresis, isothermal titration calorimetry, differential scanning calorimetry, circular dichroism spectroscopy, fluorescence spectroscopy, absorbance spectroscopy and analytical ultracentrifugation.
Resource Type: service resource, core facility, access service resource
Keywords: ABRF, characterization of biophysical properties, screening of hit/lead compound binding, thermodynamic properties, hydrodynamic properties, biological macromolecules, proteins, nucleic acids, macromolecular association reactions, ligand-macromolecule interactions, differential scanning fluorimetry, microscale thermophoresis, isothermal titration calorimetry, differential scanning calorimetry, circular dichroism spectroscopy, fluorescence spectroscopy, absorbance spectroscopy, analytical ultracentrifugation
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