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URL: http://rgd.mcw.edu/tools/strains/strains_view.cgi?id=155260361
Proper Citation: RRID:RRRC_00968
Description: Rattus norvegicus with name SD-Tg(Sycp1-Cas9-eGFP)SynblRrrc from RGD.
Species: Rattus norvegicus
Notes: This project entailed generation of a novel rat strain in which a P2A-Cas9-T2A-EGFP coding sequence was inserted at the stop codon of the rat Sycp1 gene for co-expression of Cas9 and EGFP under the control of the Sycp1 regulatory elements. Animals express Cas9 in the testis in the spermatogonial layer and in meiosis stages with 4N DNA content. Strain catalyzes high levels of Active Genetic Gene conversion when paired with certain Active Genetic cassettes. ROBUST AND EFFICIENT ACTIVE GENETICS GENE CONVERSION IN THE RAT AND MOUSE Chenyen Lai, Oscar Alvarez, Kristen Read, Don van Fossan,Christopher M Conner, Shannon Xaing-Ru Xu, Dale O. Cowley, Valentino Gantz, David R. Webb, Kurt Jarnagin URL: Doi:10.1101/2022.08.30.505951 Rat Resource and Research Center
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Source: Rat Resource and Research Center (RRID:SCR_002044)
Description: Supplies biomedical investigators with rat models, embryonic stem cells, related reagents, and protocols they require for their research. In addition to repository, cryostorage and distribution functions, RRRC can facilitate acquisition of rat strains from other international repositories as well as provide consultation and technical training to investigators using rat models.
URL: http://www.rrrc.us/
Source Database: Rat Resource and Research Center (RRRC)
