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URL: https://sites.wustl.edu/tetrahymena/finding-strains/
Proper Citation: RRID:TSC_SD03309
Description: Tetrahymena thermophila with name CU428/fsNEO from TSC.
Species: Tetrahymena thermophila
Notes: NEO coding sequence with two frameshift mutations in RPL29 vector (conferring resistance to Cyclohexamide, but remaining sensitive to paromomycin). The intention is this will provide the OLD MAC with a (defective) copy of the resistance gene, preventing its elimination in the NEW MAC after MIC transformation. From the Winey Lab at the University of Colorado Boulder.
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Source: Tetrahymena Stock Center (RRID:SCR_008362)
Description: Centralized repository and distribution site for variety of Tetrahymena strains and species. Maintains diverse array of wild type, mutant, and genetically engineered strains of T. thermophila, the most commonly used laboratory species, and variety of other species derived from both laboratory maintained stocks and wild isolates. All stocks are stored in liquid nitrogen to maintain genetic integrity and prevent senescence. In addition to providing worldwide access to strains currently in collection, TSC continually upgrades collection by accepting deposition of newly developed laboratory strains and well characterized wild isolates collected from clearly defined natural sites.
URL: https://tetrahymena.vet.cornell.edu/
Source Database: TSC, Tetrahymena Stock Center