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http://antibodyregistry.org/AB_2721146

Comments: Applications: WB, IHC
Host Organism: rabbit
Clonality: polyclonal
Target(s): WWTR1

Proper citation: ABclonal Cat# A8202, RRID:AB_2721146 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721197

Comments: Originally developed by Dr. Lester I. Binder at the University of Alabama---Original Report: The distribution of tau in the mammalian central nervous system. Binder LI, Frankfurter A, Rebhun LI. J Cell Biol. 1985 Oct;101(4):1371-8. PMID:3930508; PMCID: PMC2113928.Dephosphorylation dependence: Phosphorylation determines two distinct species of Tau in the central nervous system. Papasozomenos SC, Binder LI. . Cell Motil Cytoskeleton. 1987;8(3):210-26. PMID: 2446784.; Neurofibrillary tangles of Alzheimer disease share antigenic determinants with the axonal microtubule-associated protein tau. Wood JG, Mirra SS, Pollock NJ, Binder LI. Proc Natl Acad Sci U S A. 1986 Jun;83(11):4040-3. PMID: 2424015, PMCID: PMC323661.; Abnormal phosphorylation of the microtubule-associated protein tau in Alzheimer cytoskeletal pathologyGrundke-Iqbal I, Iqbal K, Tung YC, Quinlan M, Wisniewski HM, Binder LI. Proc Natl Acad Sci U S A. 1986. 83(13):4913-7. PMID: 3088567, PMCID: PMC323854.Epitope: Application of synthetic phospho- and unphospho- peptides to identify phosphorylation sites in a subregion of the tau molecule, which is modified in Alzheimer's disease.Liu WK, Moore WT, Williams RT, Hall FL, Yen SH. J Neurosci Res. 1993 Feb 15;34(3):371-6. PMID: 8455212; The structural basis of monoclonal antibody Alz50's selectivity for Alzheimer's disease pathologyCarmel G, Mager EM, Binder LI, Kuret J. . J Biol Chem. 1996. 271(51):32789-95. PMID: 8955115. Epitopes that span the tau molecule are shared with paired helical filamentsKosik KS, Orecchio LD, Binder L, Trojanowski JQ, Lee VM, Lee G. . Neuron. 1988. 1(9):817-25. PMID: 2483104.Monoclonal Antibody Selection and Production: BALB/c mice were immunized with phosphocellulose-purified (51) bovine MAPs, which were denatured by boiling in 0.1% SDS. Immunization was performed over a period of 2 me, with the initial injection performed in Freund's complete adjuvant and subsequent injections (at 2-wk intervals) carried out using Freund's incomplete adjuvant. Each injection contained at least 0.5 mg SDSdenatured bovine MAPs administered at both an interperitoneal and a subcutaneous site. 5 d before fusion, a final injection was administered to two animals. Immunized spleens were dissected and dissociated, and the splenocytes were fused with SP2/o myeloma cells by the procedure of Kohler and Milstein (25, 26) as modified (49). The resultant hybrid cells were plated in 12 96-well culture dishes on a thymocyte feeder layer in RPMI medium supplemented with 15% horse serum, and 24 h after plating this medium was exchanged with selection medium containing hypoxanthine, aminopterin, thymidine. Clone formation was observed within 7 d and most wells were confluent 14 d after fusion. Of the 1,156 wells plated, 1,155 were positive for hybrid formation. Antibody production was assayed by enzyme-linked immunosorbant assay (ELISA) (50) (see below, Quantitative ELISA) using phosphocellulose MAPs in the solid phase. Of the 1,155 wells assayed, >800 were positive for anti-MAP antibody production. 156 of the most intensely positive clones were picked and expanded into l-ml cultures. Upon reaching confluence, the individual clones were frozen in complete medium that contained 25% horse serum and 10% dimethyl sulfoxide. Aliquots of spent medium from each clone were tested by ELISA for binding to purified bovine MAP2 or tau. Of the 156 clones frozen, 31 expressed antibody to MAP2 whereas only 6 produced antibody that recognized bovine tau. The antibody-containing media from these six putative tau clones were tested on immunoblots of whole rat brain. Only one line, Taul, was cross-reactive in rat brain, exhibiting binding to several polypeptides in the tau region of the gel. This line was thawed and subcloned twice before injection into a BALB/c mouse for the production of antibody-containing ascites fluid. Cells obtained from sterilely drained ascites fluid were harvested by centrifugation, cultured, and subcloned once more before freezing. I 1 ml ascites fluid was harvested from one mouse and chromatographed on protein A-Sepharose, yielding nearly 30 mg of Tau-l antibody at a final concentration of 0.96 mg/ml. Except for fluctuation analysis, this affinity-purified antibody was used in all of the biochemical experiments described below. Immunocytochemistry was performed using spent tissue culture medium harvested from stationary phase Tau-l-producing hybridomas.
Host Organism: mouse
Clonality: monoclonal
Target(s): Tau protein

Proper citation: Nicholas M. Kanaan at Michigan State University Cat# Tau1, RRID:AB_2721197 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721189

Comments: Applications: Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
Host Organism: rabbit
Clonality: monoclonal
Target(s): Ki67

Proper citation: BIOCARE MEDICAL Cat# CRM 325, RRID:AB_2721189 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721100

Comments: Applications: WB,IHC
Host Organism: guinea pig
Clonality: monoclonal
Target(s): Glycine receptor; GlyR

Proper citation: Synaptic Systems Cat# 146 308, RRID:AB_2721100 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2720960

Comments: Applications: ICC/IF, WB
Host Organism: rabbit
Clonality: polyclonal
Target(s): RPS6KL1

Proper citation: Thermo Fisher Scientific Cat# PA5-77233, RRID:AB_2720960 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721082

Comments: Applications: WB,ICC,IHC,IHC-P. KO validated
Host Organism: guinea pig
Clonality: polyclonal
Target(s): Synapsin 1

Proper citation: Synaptic Systems Cat# 106 104, RRID:AB_2721082 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2720964

Comments: Applications: WB (1:500-1:2,000)
Host Organism: rabbit
Clonality: polyclonal
Target(s): PPP3R2

Proper citation: Thermo Fisher Scientific Cat# PA5-77237, RRID:AB_2720964 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721009

Comments: Applications: ChIP, Western Blotting, ELISA, Immunohistochemistry, Gel Shift
Host Organism: rabbit
Clonality: polyclonal
Target(s): NFKB p65

Proper citation: Diagenode Cat# C15310256, RRID:AB_2721009 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721242

Comments: suggested use: WB, IHC-P, ICC/IF, Flow Cyt
Host Organism: rabbit
Clonality: monoclonal
Target(s): KDM4B

Proper citation: Abcam Cat# ab191434, RRID:AB_2721242 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721140

Comments: Originating manufacturer of this product.
Applications: WB, IP, FC, , ELISA
Host Organism: rabbit
Clonality: polyclonal
Target(s): CD68

Proper citation: Proteintech Cat# 25747-1-AP, RRID:AB_2721140 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2720957

Comments: Applications: WB
Host Organism: rabbit
Clonality: polyclonal
Target(s): RPGRIP1

Proper citation: Thermo Fisher Scientific Cat# PA5-77230, RRID:AB_2720957 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2720945

Comments: Discontinued: 2023; Applications: WB (1:500-1:2,000), ICC/IF (1:50-1:100)
Host Organism: rabbit
Clonality: polyclonal
Target(s): SULT1E1

Proper citation: Thermo Fisher Scientific Cat# PA5-77218, RRID:AB_2720945 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721070

Comments: Applications: WB; IHC; ICC; IP
Host Organism: rabbit
Clonality: polyclonal
Target(s): Asporin / ASPN

Proper citation: Cloud-Clone Corp Cat# PAC321Mu01, RRID:AB_2721070 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2720982

Comments: Discontinued: 2023; Applications: IHC (P) (1:50-1:200), WB (1:500-1:1,000)
Host Organism: rabbit
Clonality: polyclonal
Target(s): NOX2

Proper citation: Thermo Fisher Scientific Cat# PA5-77255, RRID:AB_2720982 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2720969

Comments: Applications: WB (1:500-1:2,000)
Host Organism: rabbit
Clonality: polyclonal
Target(s): ELMOD3

Proper citation: Thermo Fisher Scientific Cat# PA5-77242, RRID:AB_2720969 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721199

Comments: Applications: WB, ICC, IP, IHC
Host Organism: rabbit
Clonality: polyclonal
Target(s): TrkB

Proper citation: Millipore Cat# ABN1381, RRID:AB_2721199 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721090

Comments: Applications: WB,IP,ICC,IHC,IHC-P,ELISA
Host Organism: mouse
Clonality: monoclonal
Target(s): GFAP

Proper citation: Synaptic Systems Cat# 173 211, RRID:AB_2721090 Copy   

•   Source: Antibody Registry

Discontinued

http://antibodyregistry.org/AB_2721027

Comments: Discontinued: 2017; Suitable for: WB, ICC/IF, IHC-P

Host Organism: rabbit
Clonality: monoclonal
Target(s): KLF4

Proper citation: Abcam Cat# ab151733, RRID:AB_2721027 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721081

Comments: Applications: WB,IHC,IHC-P
Host Organism: guinea pig
Clonality: polyclonal
Target(s): DARPP 32

Proper citation: Synaptic Systems Cat# 382 004, RRID:AB_2721081 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721042

Comments: Suitable for: IHC-P, WB.
Cuffe JS et al. Prenatal corticosterone exposure programs sex-specific adrenal adaptations in mouse offspring. J Endocrinol 232:37-48 (2017).
Host Organism: rabbit
Clonality: polyclonal
Target(s): CYP11A1

Proper citation: Abcam Cat# ab175408, RRID:AB_2721042 Copy   

•   Source: Antibody Registry