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http://antibodyregistry.org/AB_2762678

Comments: Applications: Flow, ICC/IF, IHC (F)
Host Organism: rat
Clonality monoclonal
Target(s): GFAP

Proper citation: (Thermo Fisher Scientific Cat# 51-9792-82, RRID:AB_2762678) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2665514

Comments: The monoclonal antibody (Clone 0518B7; Monoclonal Antibodies, Inc., Mountain View, CA; Available from Dr. Janet Roser, Department of Animal Science, University of California, Davis, CA) with specificity for bovine LH (bLH) was produced by a stable clone of cell hybrids isolated from fusions of Balb/c mouse spleen lymphocytes with NS-1 myeloma cells (5). Briefly, balb/c mice were immunized over a period of several weeks by intraperitoneal injections with bovine LH (Accurate Chemicals, Westbury, N.Y.) in complete Freund's adjuvant.
Host Organism: mouse
Clonality monoclonal
Target(s): LH

Proper citation: (Dr. Janet Roser, Department of Animal Science, University of California, Davis Cat# 518B7, RRID:AB_2665514) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2687964

Comments: Vendor recommended applications: IHC-P, Western blot, ELISA
Host Organism: goat
Clonality polyclonal
Target(s): Septin 6

Proper citation: (LSBio (LifeSpan) Cat# LS-B9200, RRID:AB_2687964) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2687987

Comments: Raised against amino acids 301-600 mapping within an internal region of VPS41 of human origin. Vendor recommended applications: WB, IP, IF, IHC-P and ELISA
Host Organism: mouse
Clonality monoclonal
Target(s): VPS41

Proper citation: (Santa Cruz Biotechnology Cat# sc-377118, RRID:AB_2687987) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2687565

Comments:
Host Organism: rabbit
Clonality polyclonal
Target(s): COL1A1

Proper citation: (Antibody Verify Cat# AAS99599C, RRID:AB_2687565) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2687575

Comments:
Host Organism: goat
Clonality polyclonal
Target(s): PAX3

Proper citation: (Sigma-Aldrich Cat# SAB2502032, RRID:AB_2687575) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2687555

Comments:
Host Organism: mouse
Clonality monoclonal
Target(s): hnRNP E1

Proper citation: (Santa Cruz Biotechnology Cat# sc-393076, RRID:AB_2687555) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2687457

Comments: Image validation for IHC-P, WB in MDS.
Host Organism: rabbit
Clonality polyclonal
Target(s): DDR2

Proper citation: (Novus Cat# NBP2-14926, RRID:AB_2687457) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2848184

Comments: Applications: WB
Host Organism: rabbit
Clonality polyclonal
Target(s): ZFP95

Proper citation: (Novus Cat# NBP2-20947, RRID:AB_2848184) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2847870

Comments: Applications: Mass Cytometry
Host Organism:
Clonality monoclonal
Target(s): Histone 3

Proper citation: (Standard BioTools Cat# 3176016A, RRID:AB_2847870) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2833244

Comments: Applications: ELISA
Kit contains: Two Anti-Bovine IL-1ß antibodies.
Note: Kit contents can vary - use with caution.
Host Organism:
Clonality unknown
Target(s): IL-1 beta

Proper citation: (Thermo Fisher Scientific Cat# ESS0027, RRID:AB_2833244) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2832208

Comments: Applications: immunohistochemistry, western blot
Host Organism: mouse
Clonality monoclonal
Target(s): VSNL1

Proper citation: (Sigma-Aldrich Cat# MABN762, RRID:AB_2832208) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2820319

Comments: Applications: WB, IHC, ICC/IF, IP, FCM, IEM
Host Organism: mouse
Clonality monoclonal
Target(s): HSP70/HSC70

Proper citation: (StressMarq Biosciences Cat# SMC-104B-DY405, RRID:AB_2820319) Copy   

•   Source: Antibody Registry

Discontinued

http://antibodyregistry.org/AB_155884

Comments: Discontinued; Applications: WB (1:500-1:5,000), IHC (1:2,000-1:10,000), IP (2-8 µg/mg lysate)
Host Organism: rabbit
Clonality unknown
Target(s): AMPK alpha 1

Proper citation: (Thermo Fisher Scientific Cat# A300-507A, RRID:AB_155884) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2756886

Comments:
Host Organism: mouse
Clonality unknown
Target(s): Luteinizing hormone beta subunit

Proper citation: (Pablo Ross; UC Davis Cat# 518B7, RRID:AB_2756886) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721197

Comments: Originally developed by Dr. Lester I. Binder at the University of Alabama---Original Report: The distribution of tau in the mammalian central nervous system. Binder LI, Frankfurter A, Rebhun LI. J Cell Biol. 1985 Oct;101(4):1371-8. PMID:3930508; PMCID: PMC2113928.Dephosphorylation dependence: Phosphorylation determines two distinct species of Tau in the central nervous system. Papasozomenos SC, Binder LI. . Cell Motil Cytoskeleton. 1987;8(3):210-26. PMID: 2446784.; Neurofibrillary tangles of Alzheimer disease share antigenic determinants with the axonal microtubule-associated protein tau. Wood JG, Mirra SS, Pollock NJ, Binder LI. Proc Natl Acad Sci U S A. 1986 Jun;83(11):4040-3. PMID: 2424015, PMCID: PMC323661.; Abnormal phosphorylation of the microtubule-associated protein tau in Alzheimer cytoskeletal pathologyGrundke-Iqbal I, Iqbal K, Tung YC, Quinlan M, Wisniewski HM, Binder LI. Proc Natl Acad Sci U S A. 1986. 83(13):4913-7. PMID: 3088567, PMCID: PMC323854.Epitope: Application of synthetic phospho- and unphospho- peptides to identify phosphorylation sites in a subregion of the tau molecule, which is modified in Alzheimer's disease.Liu WK, Moore WT, Williams RT, Hall FL, Yen SH. J Neurosci Res. 1993 Feb 15;34(3):371-6. PMID: 8455212; The structural basis of monoclonal antibody Alz50's selectivity for Alzheimer's disease pathologyCarmel G, Mager EM, Binder LI, Kuret J. . J Biol Chem. 1996. 271(51):32789-95. PMID: 8955115. Epitopes that span the tau molecule are shared with paired helical filamentsKosik KS, Orecchio LD, Binder L, Trojanowski JQ, Lee VM, Lee G. . Neuron. 1988. 1(9):817-25. PMID: 2483104.Monoclonal Antibody Selection and Production: BALB/c mice were immunized with phosphocellulose-purified (51) bovine MAPs, which were denatured by boiling in 0.1% SDS. Immunization was performed over a period of 2 me, with the initial injection performed in Freund's complete adjuvant and subsequent injections (at 2-wk intervals) carried out using Freund's incomplete adjuvant. Each injection contained at least 0.5 mg SDSdenatured bovine MAPs administered at both an interperitoneal and a subcutaneous site. 5 d before fusion, a final injection was administered to two animals. Immunized spleens were dissected and dissociated, and the splenocytes were fused with SP2/o myeloma cells by the procedure of Kohler and Milstein (25, 26) as modified (49). The resultant hybrid cells were plated in 12 96-well culture dishes on a thymocyte feeder layer in RPMI medium supplemented with 15% horse serum, and 24 h after plating this medium was exchanged with selection medium containing hypoxanthine, aminopterin, thymidine. Clone formation was observed within 7 d and most wells were confluent 14 d after fusion. Of the 1,156 wells plated, 1,155 were positive for hybrid formation. Antibody production was assayed by enzyme-linked immunosorbant assay (ELISA) (50) (see below, Quantitative ELISA) using phosphocellulose MAPs in the solid phase. Of the 1,155 wells assayed, >800 were positive for anti-MAP antibody production. 156 of the most intensely positive clones were picked and expanded into l-ml cultures. Upon reaching confluence, the individual clones were frozen in complete medium that contained 25% horse serum and 10% dimethyl sulfoxide. Aliquots of spent medium from each clone were tested by ELISA for binding to purified bovine MAP2 or tau. Of the 156 clones frozen, 31 expressed antibody to MAP2 whereas only 6 produced antibody that recognized bovine tau. The antibody-containing media from these six putative tau clones were tested on immunoblots of whole rat brain. Only one line, Taul, was cross-reactive in rat brain, exhibiting binding to several polypeptides in the tau region of the gel. This line was thawed and subcloned twice before injection into a BALB/c mouse for the production of antibody-containing ascites fluid. Cells obtained from sterilely drained ascites fluid were harvested by centrifugation, cultured, and subcloned once more before freezing. I 1 ml ascites fluid was harvested from one mouse and chromatographed on protein A-Sepharose, yielding nearly 30 mg of Tau-l antibody at a final concentration of 0.96 mg/ml. Except for fluctuation analysis, this affinity-purified antibody was used in all of the biochemical experiments described below. Immunocytochemistry was performed using spent tissue culture medium harvested from stationary phase Tau-l-producing hybridomas.
Host Organism: mouse
Clonality monoclonal
Target(s): Tau protein

Proper citation: (Nicholas M. Kanaan at Michigan State University Cat# Tau1, RRID:AB_2721197) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2716731

Comments: Applications: immunoblotting
Host Organism: rabbit
Clonality polyclonal
Target(s): PCDH17

Proper citation: (Sigma-Aldrich Cat# SAB2108080, RRID:AB_2716731) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2716813

Comments: Originating manufacturer of this product.
Applications: WB, IP, IHC, IF, FC, ELISA
Host Organism: rabbit
Clonality polyclonal
Target(s): CD45

Proper citation: (Proteintech Cat# 20103-1-AP, RRID:AB_2716813) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2716755

Comments: Originating manufacturer of this product.
Applications: WB, IHC, IF, FC, ELISA
Host Organism: rabbit
Clonality polyclonal
Target(s): Histone-H3

Proper citation: (Proteintech Cat# 17168-1-AP, RRID:AB_2716755) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2716452

Comments: Originating manufacturer of this product. Applications: WB, IHC. This antibody reacts to Akt only when phosphorylated at Ser473. There is no cross-reactivity with Akt without phosphorylation at Ser473. This antibody may also react to bovine, mouse or rat Phospho-Akt (Ser473), as predicted by immunogen homology
Host Organism: rabbit
Clonality unknown
Target(s): Phospho-Akt (Ser473)

Proper citation: (RevMAb Biosciences Cat# 31-1131-00, RRID:AB_2716452) Copy   

•   Source: Antibody Registry