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Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572268

Comments: manufacturer recommendations: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Immunohistochemistry; Immunocytochemistry; Immunofluorescence
Consolidated with AB_2313787 on 10/10/16
Host Organism: mouse
Clonality monoclonal
Target(s): Tyrosine Hydroxylase

Proper citation: (ImmunoStar Cat# 22941, RRID:AB_572268) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572250

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The antibody has a proven strong indirect immunofluorescent staining at a 1/400-1/600 dilution and a proven 4+ biotin-streptavidin/HRP staining at 1/1,000-1/1,200 dilution in rat globus pallidus and amygdala. Staining is completely eliminated by pretreatment with 100 µg of methionine enkephalin per mL of diluted antiserum. Pretreatment with 100 µg of leucine enkephalin only partially blocks staining.; Gene Symbol: Fas
Host Organism: rabbit
Clonality polyclonal
Target(s): Methionine Enkephalin

Proper citation: (ImmunoStar Cat# 20065, RRID:AB_572250) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572256

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The neuronal nitric oxide synthase C-terminal antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 - 1/1,500 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/8,000 - 1/12,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immuno-labeling is completely abolished by pre-adsorption with synthetic human nNOS (1419-1433) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS were observed. The nNOS antiserum has been used successfully in human, rat, mouse, guinea pig, cat, and monkey tissue. Detection of nNOS from other species will depend upon sequence homology.; Gene Symbol:
Host Organism: rabbit
Clonality polyclonal
Target(s): Human nNOS (1419-1433)

Proper citation: (ImmunoStar Cat# 24287, RRID:AB_572256) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572248

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The antibody produces a strong postive labeling of LHRH at dilutions of 1/200-1/400 using indirect immunofluorescence and at dilutions of 1/2,000 - 1,4,000 using biotin-streptavidin/HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Staining is completely eliminated by pretreatment of the diluted antibody with 5 µg of LHRH per mL of diluted antiserum.; Gene Symbol: NELF
Host Organism: rabbit
Clonality polyclonal
Target(s): Luteinizing Hormone Releasing Hormone

Proper citation: (ImmunoStar Cat# 20075, RRID:AB_572248) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572251

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The Mu Opioid Receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat caudate putamen and spinal cord (dorsal horn) using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 - 1/1000 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/6000 - 1/10000 in PBS/0.3% Triton X-100 - Biotin/avidin-HRP Technique. Preadsorption with MOR peptide (384-398) at 10 µg/ml completely eliminates labeling. The specificity of the antiserum was determined by immunolabeling of transfected cells, Western Blot analysis and immunoisolation studies.; Gene Symbol:
Host Organism: rabbit
Clonality polyclonal
Target(s): Synthetic rat MOR1 (384-398)

Proper citation: (ImmunoStar Cat# 24216, RRID:AB_572251) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572266

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The Substance P antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat substantia nigra and spinal cord using biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500-1/1000 in PBS/0.3% Triton X-100 - Cy3 Fluorchrome and 1/6000-1/8000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. The specificity of the antiserum for Substance P was demonstrated using soluble pre-adsorption with the peptides in question at a final concentration of 10 µg of peptide per mL of diluted antiserum. Substance P immunolabeling was completely abolished by pre-adsorption with Substance P. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: neurokinin A, neurokinin B, somatostatin and neuropeptide K.; Gene Symbol: Tacr1
Host Organism: rabbit
Clonality polyclonal
Target(s): Substance P

Proper citation: (ImmunoStar Cat# 20064, RRID:AB_572266) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572219

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The antibody has a proven strong fluorescent staining at a 1/400 - 1/800 dilution and a proven strong biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat hypothalamus. Staining is completely eliminated by pretreatment of the diluted antibody with 10 µg/mL of arginine vasopressin. Pre-adsorption with as much as 100 µg/mL of oxytocin had no effect on immunolabeling.; Gene Symbol: AVPR2
Host Organism: rabbit
Clonality polyclonal
Target(s): Arg Vasopressin

Proper citation: (ImmunoStar Cat# 20069, RRID:AB_572219) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572240

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, This antibody has been shown to react strongly with human GFAP as well as with GFAP from rat, mouse, guinea pig, hamster, kangaroo, sheep, cat and monkey. Excellent staining results were obtained when rabbit anti-glial fibrillary acidic protein serum was tested.; Gene Symbol: IL6ST
Host Organism: rabbit
Clonality polyclonal
Target(s): Glial Fibrillary Acid Protein

Proper citation: (ImmunoStar Cat# 22522, RRID:AB_572240) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572227

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The antibody has a proven strong staining at a 1/1000 - 1/2000 dilution in rat adrenal medulla using Biotin-Streptavidin/HRP detection method.; Gene Symbol: VEGFA; Rated by ISCC, Intestinal Stem Cell Consortium (check ratings https://iscc.coh.org/)
Host Organism: rabbit
Clonality polyclonal
Target(s): SP-1 Chromogranin A

Proper citation: (ImmunoStar Cat# 20085, RRID:AB_572227) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572262

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT Goat Rabbit Antibody was raised against serotonin coupled to BSA with paraformaldehyde. The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/400 - 1/800 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/5000 - 1/10,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg of serotonin/BSA conjugate.; Gene Symbol: Spl; Rated by ISCC, Intestinal Stem Cell Consortium (check ratings https://iscc.coh.org/)
Host Organism: goat
Clonality polyclonal
Target(s): Serotonin

Proper citation: (ImmunoStar Cat# 20079, RRID:AB_572262) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572270

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The antibody has a proven strong fluorescent staining at a 1/200-1/400 dilution and a strong Biotin-Streptavidin/HRP staining at a 1/4000-1/6000 dilution in rat amygdala, cortex, and suprachiasmatic nucleus. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-5 M. VIP immunolabeling was completely abolished by pre-adsorption with VIP. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: Secretin, gastric inhibitory polypeptide, somatostatin, glucagon, insulin, ACTH, gastrin 34, FMRF-amide, rat GHRF, human GHRF, peptide histidine isoleucine 27, rat pancreatic polypeptide, motilin, peptide YY, substance P, neuropeptide Y, and CGRP. The histochemical antibody for VIP is generated in a rabbit against porcine VIP conjugated to bovine thyroglobulin with carbodiimide. The antibody is provided as 100 µL of lyophilized whole serum, and 0.09% sodium azide.; Gene Symbol: VIP
Host Organism: rabbit
Clonality polyclonal
Target(s): Vasoactive Intestinal Peptide

Proper citation: (ImmunoStar Cat# 20077, RRID:AB_572270) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572259

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry; Note, The antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat thalamus, cortex, and hippocampus. The antiserum has been characterized as specific to parvalbumin; please see reference listed below. Recommended primary dilutions are 1/400 - 1/800 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/5,000 - 1/8,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique.; Gene Symbol: Myh6
Host Organism: rabbit
Clonality polyclonal
Target(s): Rat muscle paralbumin

Proper citation: (ImmunoStar Cat# 24428, RRID:AB_572259) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572245

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon. The Histamine antiserum has a sensitivity level capable of detecting the low level Histamine contents of the brain. The Histamine antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 - 1/1000 in PBS/0.3% Triton X-100 Cy3 Technique and 1/4000-1/6000 in PBS/0.3% Triton X-100 biotin/avidin-HRP Technique . All staining is blocked by preabsorption of the antiserum with Histamine conjugate. Cross reactivity experiments indicate no cross reactivity with L-histidine or L-histidine containing peptides such as LH-RH.; Gene Symbol: Hrh1
Host Organism: rabbit
Clonality polyclonal
Target(s): Histamine

Proper citation: (ImmunoStar Cat# 22939, RRID:AB_572245) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572261

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, This product consists of the immunoglobulin fraction of rabbit anti-S-100 serum. The antibody was generated in rabbits against S-100 protein isolated from bovine brain. Common determinants on this protein occur amongst widely disparate species, e.g. fruit fly and man. Anti-S-100 reacts with the alpha and beta subunits of S-100A. In the central nervous system, glial and ependymal cells stain positively. In the peripheral nervous system, Schwann cells are positively labeled. Melanocytes and Langerhans cells stain in skin.; Gene Symbol: s100
Host Organism: rabbit
Clonality polyclonal
Target(s): S100

Proper citation: (ImmunoStar Cat# 22520, RRID:AB_572261) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572244

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The Glutamate Antibody was raised to glutamate coupled to KLH with glutaraldehyde. The antibody produces strong labeling of glutamate at dilutions of 1/2,000 - 1/4,000 using biotin-streptavidin/HRP technique. Glutamate tissue staining is completely eliminated by preincubation with glutamate conjugate at concentrations of 100 µg conjugate per mL of diluted antiserum. Aspartate and glutamine conjugates could not significantly inhibit tissue staining. The following amino acids were tested for cross reactivity using an enzyme-linked immunoassay method. Wells were coated with Glu-Glut-Btg at 1 µg per mL. Amino acids and conjugate were added at concentrations from 10 µg to 1 ng per mL. The Glutamate antibody was added to wells at 1 µg per mL. These amino acids were found to have cross reactivity at less than 1%: Beta-alanine, L-alanine, L-aspartic acid, L-glutamic acid, Glycine, Taurine and L-tyrosine.; Gene Symbol: GRIK3
Host Organism: mouse
Clonality monoclonal
Target(s): Glutamate

Proper citation: (ImmunoStar Cat# 22523, RRID:AB_572244) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572247

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The antibody has a proven strong indirect immunofluorescent staining at a 1/400 - 1/600 dilution and a proven strong biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat globus pallidus and spinal cord. Staining is completely eliminated by pretreatment with 50 µg of Leucine Enkephalin per mL of diluted antiserum. Pretreatment with 50 µg of Methionine Enkephalin per mL of diluted antiserum also significantly blocks staining.; Gene Symbol:
Host Organism: rabbit
Clonality polyclonal
Target(s): Leucine Enkephalin

Proper citation: (ImmunoStar Cat# 20066, RRID:AB_572247) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572258

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The Oxytocin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/200 - 1/400 in PBS/0.3% Triton X-100 - FITC and 1/4,000 - 1/8,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP. Staining is completely eliminated by pretreatment of 1 mL of the diluted antibody with 5 µg of Oxytocin. Pretreatment of 1 mL of the diluted antibody with as much as 100 µg of vasopressin does not diminish staining.; Gene Symbol: Oxt
Host Organism: rabbit
Clonality polyclonal
Target(s): Oxytocin

Proper citation: (ImmunoStar Cat# 20068, RRID:AB_572258) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572246

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pancreatic beta cells using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions for these methods are 1/500-1/1000 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/3000-1/5000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Immuostaining is completely abolished by soluble pre-adsorption with bovine insulin at 10 µg per mL of diluted antiserum.; Gene Symbol: PIN
Host Organism: rabbit
Clonality polyclonal
Target(s): Insulin

Proper citation: (ImmunoStar Cat# 20056, RRID:AB_572246) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572254

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The Neurotensin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat amygdala using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/200-1/400 in PBS/0.3% Triton x-100 - Cy3 Technique and 1/4000-1/8000 in PBS/0.3% Triton x-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment with 10 µg of Neurotensin per 1 mL of diluted antibody.; Gene Symbol: Nrtim2
Host Organism: rabbit
Clonality polyclonal
Target(s): Neurotensin

Proper citation: (ImmunoStar Cat# 20072, RRID:AB_572254) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572218

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The Alpha-MSH Antibody was raised to synthetic human a-MSH coupled to bovine thyroglobulin with glutaraldehyde. The ImmunoStar alpha melanocyte stimulating hormone antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pituitary using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100-1/200 in PBS/0.3% Triton X-100 - FITC Technique and 1/4000-1/6000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 ug/mL of alpha-MSH.; Gene Symbol: alpha-msh
Host Organism: rabbit
Clonality polyclonal
Target(s): alpha-MSH

Proper citation: (ImmunoStar Cat# 20074, RRID:AB_572218) Copy   

•   Source: Antibody Registry