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Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572268

Comments: manufacturer recommendations: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Immunohistochemistry; Immunocytochemistry; Immunofluorescence
Consolidated with AB_2313787 on 10/10/16
Host Organism: mouse
Clonality monoclonal
Target(s): Tyrosine Hydroxylase

Proper citation: (ImmunoStar Cat# 22941, RRID:AB_572268) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572250

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The antibody has a proven strong indirect immunofluorescent staining at a 1/400-1/600 dilution and a proven 4+ biotin-streptavidin/HRP staining at 1/1,000-1/1,200 dilution in rat globus pallidus and amygdala. Staining is completely eliminated by pretreatment with 100 µg of methionine enkephalin per mL of diluted antiserum. Pretreatment with 100 µg of leucine enkephalin only partially blocks staining.; Gene Symbol: Fas
Host Organism: rabbit
Clonality polyclonal
Target(s): Methionine Enkephalin

Proper citation: (ImmunoStar Cat# 20065, RRID:AB_572250) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572248

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The antibody produces a strong postive labeling of LHRH at dilutions of 1/200-1/400 using indirect immunofluorescence and at dilutions of 1/2,000 - 1,4,000 using biotin-streptavidin/HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Staining is completely eliminated by pretreatment of the diluted antibody with 5 µg of LHRH per mL of diluted antiserum.; Gene Symbol: NELF
Host Organism: rabbit
Clonality polyclonal
Target(s): Luteinizing Hormone Releasing Hormone

Proper citation: (ImmunoStar Cat# 20075, RRID:AB_572248) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572266

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The Substance P antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat substantia nigra and spinal cord using biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500-1/1000 in PBS/0.3% Triton X-100 - Cy3 Fluorchrome and 1/6000-1/8000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. The specificity of the antiserum for Substance P was demonstrated using soluble pre-adsorption with the peptides in question at a final concentration of 10 µg of peptide per mL of diluted antiserum. Substance P immunolabeling was completely abolished by pre-adsorption with Substance P. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: neurokinin A, neurokinin B, somatostatin and neuropeptide K.; Gene Symbol: Tacr1
Host Organism: rabbit
Clonality polyclonal
Target(s): Substance P

Proper citation: (ImmunoStar Cat# 20064, RRID:AB_572266) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572217

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The CGRP Antibody was raised to rat alpha-CGRP coupled to bovine thyroglobulin with glutaraldehyde. The antibody has a proven fluorescein staining at a 1/100-1/200 dilution and a strong Biotin-Streptadvidin/HRP staining at a 1/2000-1/4000 dilution in rat amygdala, and spinal cord. The specificity of the antiserum was evaluated by soluble pre-adsorption with the peptides in question at a final concentration of 10-5M. CGRP immunolabeling was completely abolished by pre-adsorption with rat α -CGRP and partially eliminated by pre-adsorption with rat
Host Organism: rabbit
Clonality polyclonal
Target(s): rat alpha-CGRP

Proper citation: (ImmunoStar Cat# 24112, RRID:AB_572217) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572227

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The antibody has a proven strong staining at a 1/1000 - 1/2000 dilution in rat adrenal medulla using Biotin-Streptavidin/HRP detection method.; Gene Symbol: VEGFA; Rated by ISCC, Intestinal Stem Cell Consortium (check ratings https://iscc.coh.org/)
Host Organism: rabbit
Clonality polyclonal
Target(s): SP-1 Chromogranin A

Proper citation: (ImmunoStar Cat# 20085, RRID:AB_572227) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572226

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The antibody has a proven strong immunofluorescent staining at a 1/200-1/400 dilution, and a 4+ Biotin-Streptavidin/HRP staining at a 1/500-1/1000 dilution, in rat adrenal medulla and rat stomach.; Gene Symbol: CHGA; Rated by ISCC, Intestinal Stem Cell Consortium (check ratings https://iscc.coh.org/)
Host Organism: rabbit
Clonality polyclonal
Target(s): porcine Chromogranin A

Proper citation: (ImmunoStar Cat# 20086, RRID:AB_572226) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572262

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT Goat Rabbit Antibody was raised against serotonin coupled to BSA with paraformaldehyde. The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/400 - 1/800 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/5000 - 1/10,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg of serotonin/BSA conjugate.; Gene Symbol: Spl; Rated by ISCC, Intestinal Stem Cell Consortium (check ratings https://iscc.coh.org/)
Host Organism: goat
Clonality polyclonal
Target(s): Serotonin

Proper citation: (ImmunoStar Cat# 20079, RRID:AB_572262) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572270

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The antibody has a proven strong fluorescent staining at a 1/200-1/400 dilution and a strong Biotin-Streptavidin/HRP staining at a 1/4000-1/6000 dilution in rat amygdala, cortex, and suprachiasmatic nucleus. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-5 M. VIP immunolabeling was completely abolished by pre-adsorption with VIP. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: Secretin, gastric inhibitory polypeptide, somatostatin, glucagon, insulin, ACTH, gastrin 34, FMRF-amide, rat GHRF, human GHRF, peptide histidine isoleucine 27, rat pancreatic polypeptide, motilin, peptide YY, substance P, neuropeptide Y, and CGRP. The histochemical antibody for VIP is generated in a rabbit against porcine VIP conjugated to bovine thyroglobulin with carbodiimide. The antibody is provided as 100 µL of lyophilized whole serum, and 0.09% sodium azide.; Gene Symbol: VIP
Host Organism: rabbit
Clonality polyclonal
Target(s): Vasoactive Intestinal Peptide

Proper citation: (ImmunoStar Cat# 20077, RRID:AB_572270) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572245

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon. The Histamine antiserum has a sensitivity level capable of detecting the low level Histamine contents of the brain. The Histamine antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 - 1/1000 in PBS/0.3% Triton X-100 Cy3 Technique and 1/4000-1/6000 in PBS/0.3% Triton X-100 biotin/avidin-HRP Technique . All staining is blocked by preabsorption of the antiserum with Histamine conjugate. Cross reactivity experiments indicate no cross reactivity with L-histidine or L-histidine containing peptides such as LH-RH.; Gene Symbol: Hrh1
Host Organism: rabbit
Clonality polyclonal
Target(s): Histamine

Proper citation: (ImmunoStar Cat# 22939, RRID:AB_572245) Copy   

•   Source: Antibody Registry

Ratings or validation data are available for this resource

http://antibodyregistry.org/AB_572247

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The antibody has a proven strong indirect immunofluorescent staining at a 1/400 - 1/600 dilution and a proven strong biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat globus pallidus and spinal cord. Staining is completely eliminated by pretreatment with 50 µg of Leucine Enkephalin per mL of diluted antiserum. Pretreatment with 50 µg of Methionine Enkephalin per mL of diluted antiserum also significantly blocks staining.; Gene Symbol:
Host Organism: rabbit
Clonality polyclonal
Target(s): Leucine Enkephalin

Proper citation: (ImmunoStar Cat# 20066, RRID:AB_572247) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572246

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pancreatic beta cells using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions for these methods are 1/500-1/1000 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/3000-1/5000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Immuostaining is completely abolished by soluble pre-adsorption with bovine insulin at 10 µg per mL of diluted antiserum.; Gene Symbol: PIN
Host Organism: rabbit
Clonality polyclonal
Target(s): Insulin

Proper citation: (ImmunoStar Cat# 20056, RRID:AB_572246) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572214

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western blot, Northern blot; Note, The 5-HT7 Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 8-23 of the rat 5-HT7 receptor coupled to carrier protein with glutaraldehyde. The ImmunoStar 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS - biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended. The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.; Gene Symbol:
Host Organism: rabbit
Clonality polyclonal
Target(s): Rat 5-HT7 receptor (8-23)

Proper citation: (ImmunoStar Cat# 24430, RRID:AB_572214) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572229

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The antibody has a proven strong indirect immunofluorescence at 1/400 - 1/800 and 4+ biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat brainstem, cerebellum and adrenal medulla. Using Western blot of purified DBH the antiserum detects a triplet at approximately 72-74 kD.; Gene Symbol: DBH
Host Organism: rabbit
Clonality polyclonal
Target(s): Bovine DBH

Proper citation: (ImmunoStar Cat# 22806, RRID:AB_572229) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572235

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The antibody has a proven strong indirect immunofluorescent staining at a 1/400 - 1/800 dilution and a proven strong staining using a 1/2000-1/3000 dilution with biotin-streptavidin/HRP technique in rat stomach/antrum. The specificity of the antiserum was evaluated by soluble pre-adsorption with the peptides in question at a final concentration of 10-6 M. Gastrin-17 immunolabeling was completely abolished by pre-adsorption with Gastrin 1 and big Gastrin and partially abolished by CCK-8. Pre-adsorption with the following peptides resulted in no loss of immunostaining: motilin, secretin, PHI-27, bombesin, VIP and GIP.; Gene Symbol: GAST
Host Organism: rabbit
Clonality polyclonal
Target(s): Gastrin-17

Proper citation: (ImmunoStar Cat# 20055, RRID:AB_572235) Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_572241

Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The Glucagon Antibody was raised to glucagon coupled to bovine thyroglobulin with glutaraldehyde. The antibody has a proven strong Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1000 dilution in human pancreatic islets. Staining is completely eliminated by pretreatment of the diluted antibody in an excess of glucagon. Preadsorption of the diluted antibody with an excess of the following substances had no effect on glucagon labeling: secretin, vasoactive intestinal peptide, peptide histidine isoleucine-27, gastric inhibitory polypeptide, rat and human growth hormone releasing hormone and somatostatin.; Gene Symbol: INSGLG
Host Organism: rabbit
Clonality polyclonal
Target(s): Glucagon

Proper citation: (ImmunoStar Cat# 20076, RRID:AB_572241) Copy   

•   Source: Antibody Registry