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Genetic Insert: Codon Optimized SpCas9
Vector Backbone Description: Vector Backbone:pCM2; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:32367443
Proper citation: RRID:Addgene_217648 Copy
Vector Backbone Description: Vector Backbone:pMMB207; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39162403
Comments: Gebhardt lab ID # pMJG588.
Proper citation: RRID:Addgene_218359 Copy
Vector Backbone Description: Vector Backbone:pMMB207; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39162403
Comments: Gebhardt lab ID # pMJG120.
Proper citation: RRID:Addgene_218357 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: scbe3-NG-HF1
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218160 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: escbe3-NG
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218163 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: scbe3-HF1
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218156 Copy
Vector Backbone Description: Vector Backbone:pMMB207; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39162403
Comments: Gebhardt lab ID # pMJG599.
Proper citation: RRID:Addgene_218361 Copy
Species: Stackebrandtia nassauensis
Genetic Insert: SnaA
Vector Backbone Description: Vector Backbone:pOSV801; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:38947204
Comments: encodes biosynthetic genes for threopeptin, including SnaA, SnaB, SnaC, SnaD, SnaE, SnaO, SnaT1, SnaT2
Proper citation: RRID:Addgene_225055 Copy
Genetic Insert: apramycin resistance gene
Vector Backbone Description: Vector Backbone:pSGAb; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Proper citation: RRID:Addgene_226171 Copy
Vector Backbone Description: Vector Backbone:R6Kγ; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39302127
Comments: Tn7 expression vector.
Proper citation: RRID:Addgene_222354 Copy
Species: Synthetic
Genetic Insert: Modified Cas9 with polyaspartate linking
Vector Backbone Description: Backbone Size:7510; Vector Backbone:pCRISPomyces-2; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39833194
Comments: Depositor notes that there are SNPs in pSG5 Rep that cause the following amino acid substitutions: L468H R467Q C354W S215A K165E H144R, but these are not of concern for plasmid function.
Proper citation: RRID:Addgene_234428 Copy
Species: Other
Genetic Insert: Level 2 RECKLEEN plasmid
Vector Backbone Description: Vector Backbone:Custom built using Marburg Collection; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:41168338
Comments: Please visit https://doi.org/10.21203/rs.3.rs-5859838/v1 for preprint.
Proper citation: RRID:Addgene_233457 Copy
Species: Other
Genetic Insert: Level 2 RECKLEEN plasmid, Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4
Vector Backbone Description: Vector Backbone:Custom built using Marburg Collection; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:41168338
Comments: Please visit https://doi.org/10.21203/rs.3.rs-5859838/v1 for preprint.
Proper citation: RRID:Addgene_233458 Copy
Vector Backbone Description: Vector Backbone:pSynORI; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:41629321
Comments: J23119-Repressor (F3m1)-RNA Primer (-68nt)-Target (F3m1)-J23110-parDE-parCBA-AprR
Proper citation: RRID:Addgene_253209 Copy
Genetic Insert: SceI
Vector Backbone Description: Vector Backbone:pSW-Apr; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:36216579
Proper citation: RRID:Addgene_252219 Copy
Species: Synthetic
Genetic Insert: AmR
Vector Backbone Description: Backbone Size:6370; Vector Backbone:pFNC-6; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39778677
Proper citation: RRID:Addgene_227670 Copy
Vector Backbone Description: Backbone Size:12095; Vector Backbone:pET28a and pWH1266; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548010
Proper citation: RRID:Addgene_122001 Copy
Vector Backbone Description: Backbone Size:16599; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548010
Comments: Please note that the plasmid is prone to recombination between the duplicate tac promoter-lac operator. Addgene recommends that you test 2-3 colonies via digest or sequencing to confirm that the full 16.6kb plasmid was selected.
Proper citation: RRID:Addgene_121998 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548381
Comments: Please visit https://www.biorxiv.org/content/10.1101/582403v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_125689 Copy
Vector Backbone Description: Backbone Size:5715; Vector Backbone:pSET152; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:32972929
Proper citation: RRID:Addgene_153414 Copy
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