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Species: Synthetic
Genetic Insert: his_T_min_linker_crp_T_min double terminator
Vector Backbone Description: Backbone Size:3967; Vector Backbone:pFAB777; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: Terminator efficiency measured at 99% (97% and 73% alone, respectively).
Terminator inserted between RFP and GFP and flanked by Rnase III sites. RNase sites have sequences GTGATAGACTCAAGGTCGCTCCTAGCGAGTGGCCTTTATGATTATCAC and AGAGGGACAAACTCAAGGTCATTCGCAAGAGTGGCCTTTATGATTGACCTTCT, respectively. Terminator can be amplified by PCR with or without these sites, as desired.
Proper citation: RRID:Addgene_47860 Copy
Species: Synthetic
Genetic Insert: M13_central_T_linker_rrnD_T1 double terminator
Vector Backbone Description: Backbone Size:3967; Vector Backbone:pFAB775; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: Terminator efficiency measured at 100% (97% and 99% alone, respectively).
Terminator inserted between RFP and GFP and flanked by Rnase III sites. RNase sites have sequences GTGATAGACTCAAGGTCGCTCCTAGCGAGTGGCCTTTATGATTATCAC and AGAGGGACAAACTCAAGGTCATTCGCAAGAGTGGCCTTTATGATTGACCTTCT, respectively. Terminator can be amplified by PCR with or without these sites, as desired.
Proper citation: RRID:Addgene_47858 Copy
Species: Synthetic
Genetic Insert: ilvGEDA
Vector Backbone Description: Backbone Size:3967; Vector Backbone:pFAB768; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: Terminator efficiency measured at 99%.
Terminator inserted between RFP and GFP and flanked by Rnase III sites. RNase sites have sequences GTGATAGACTCAAGGTCGCTCCTAGCGAGTGGCCTTTATGATTATCAC and AGAGGGACAAACTCAAGGTCATTCGCAAGAGTGGCCTTTATGATTGACCTTCT, respectively. Terminator can be amplified by PCR with or without these sites, as desired.
Proper citation: RRID:Addgene_47851 Copy
Species: Synthetic
Genetic Insert: BBa_B1006 U10
Vector Backbone Description: Backbone Size:3967; Vector Backbone:pFAB774; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: Terminator efficiency measured at 99%.
Terminator inserted between RFP and GFP and flanked by Rnase III sites. RNase sites have sequences GTGATAGACTCAAGGTCGCTCCTAGCGAGTGGCCTTTATGATTATCAC and AGAGGGACAAACTCAAGGTCATTCGCAAGAGTGGCCTTTATGATTGACCTTCT, respectively. Terminator can be amplified by PCR with or without these sites, as desired.
Proper citation: RRID:Addgene_47857 Copy
Species: Synthetic
Genetic Insert: tetAC terminator
Vector Backbone Description: Backbone Size:3967; Vector Backbone:pFAB772; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: Terminator efficiency measured at 50%.
Terminator inserted between RFP and GFP and flanked by Rnase III sites. RNase sites have sequences GTGATAGACTCAAGGTCGCTCCTAGCGAGTGGCCTTTATGATTATCAC and AGAGGGACAAACTCAAGGTCATTCGCAAGAGTGGCCTTTATGATTGACCTTCT, respectively. Terminator can be amplified by PCR with or without these sites, as desired.
Proper citation: RRID:Addgene_47855 Copy
Species: Synthetic
Genetic Insert: lambda tR2 terminator
Vector Backbone Description: Backbone Size:3967; Vector Backbone:pFAB766; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: Terminator efficiency measured at 91%.
Terminator inserted between RFP and GFP and flanked by Rnase III sites. RNase sites have sequences GTGATAGACTCAAGGTCGCTCCTAGCGAGTGGCCTTTATGATTATCAC and AGAGGGACAAACTCAAGGTCATTCGCAAGAGTGGCCTTTATGATTGACCTTCT, respectively. Terminator can be amplified by PCR with or without these sites, as desired.
Proper citation: RRID:Addgene_47849 Copy
Species: Synthetic
Genetic Insert: T7 early terminator
Vector Backbone Description: Backbone Size:3967; Vector Backbone:pFAB764; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: Terminator efficiency measured at 96%.
Terminator inserted between RFP and GFP and flanked by Rnase III sites. RNase sites have sequences GTGATAGACTCAAGGTCGCTCCTAGCGAGTGGCCTTTATGATTATCAC and AGAGGGACAAACTCAAGGTCATTCGCAAGAGTGGCCTTTATGATTGACCTTCT, respectively. Terminator can be amplified by PCR with or without these sites, as desired.
Proper citation: RRID:Addgene_47847 Copy
Species: Synthetic
Genetic Insert: T3 early terminator
Vector Backbone Description: Backbone Size:3967; Vector Backbone:pFAB765; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: Terminator efficiency measured at 94%.
Terminator inserted between RFP and GFP and flanked by Rnase III sites. RNase sites have sequences GTGATAGACTCAAGGTCGCTCCTAGCGAGTGGCCTTTATGATTATCAC and AGAGGGACAAACTCAAGGTCATTCGCAAGAGTGGCCTTTATGATTGACCTTCT, respectively. Terminator can be amplified by PCR with or without these sites, as desired.
Proper citation: RRID:Addgene_47848 Copy
Species: Synthetic
Genetic Insert: promoter 13 and BCD1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: http://biofab.org/services
RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 48.98
Proper citation: RRID:Addgene_47844 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:8482; Vector Backbone:two ori derived from pUC19 and pPZP201; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21364738
Comments: level 2 vector for cloning directly from level 0
Proper citation: RRID:Addgene_48076 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4302; Vector Backbone:Backbone with three ori derived from pUC19, p15a from pACYC184 and RK2 from pBIN19; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21364738
Comments: High copy before cloning, medium copy after cloning
Proper citation: RRID:Addgene_48074 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4887; Vector Backbone:derived from pPZP200 and pUC19; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21364738
Comments: level P cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells
Proper citation: RRID:Addgene_48053 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4887; Vector Backbone:derived from pPZP200 and pUC19; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21364738
Comments: level P cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells
Proper citation: RRID:Addgene_48054 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4887; Vector Backbone:derived from pPZP200 and pUC19; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21364738
Comments: level P cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells
Proper citation: RRID:Addgene_48052 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4824; Vector Backbone:pAGM4351; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21364738
Comments: PLEASE NOTE- It has recently come to light that plasmid pAGM4723 lacks a complete overdrive sequence at the right border. This can cause incomplete TDNA insertion events and can give a higher incidence of vector backbone transfer events due to transfer initiating at the left border.
Level 2 cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells.
Please note that bacterial colonies for this plasmid often appear red in color because the plasmid contains a red color selectable marker (CRed--an artificial bacterial operon responsible for canthaxanthin biosynthesis).
Proper citation: RRID:Addgene_48015 Copy
Genetic Insert: Tshld-GFP
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4470; Vector Backbone:pDONR 201; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24615791
Comments: The Tshld system allows users to fine-tune the concentration of a protein of interest in mammalian cells by titrating the concentration of rapamycin or FK506. Unlike other post-translational techniques, the Tshld system releases its protein of interest in its native form, so it is highly attractive to be used in investigations of proteins involved in multimeric protein complexes.
Proper citation: RRID:Addgene_53211 Copy
Vector Backbone Description: Backbone Size:3504; Vector Backbone:pzDonor; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25122746
Proper citation: RRID:Addgene_53189 Copy
Vector Backbone Description: Backbone Size:3515; Vector Backbone:pzDonor; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25122746
Proper citation: RRID:Addgene_53188 Copy
Species: Rattus norvegicus
Genetic Insert: Syntaxin 1A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53236 Copy
Species: Rattus norvegicus
Genetic Insert: VAMP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53233 Copy
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