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Vector Backbone Description: Backbone Marker:ATCC 10326362; Backbone Size:5303; Vector Backbone:pENTR1A-Gent; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:16945906
Comments: shRNA expression; miR30-based topology; TRE Pmin promoter + GFP spacer; Entry vector backbone; +ccdB in parent; Multiple unique sites d/s of TRE
Proper citation: RRID:Addgene_25749 Copy
Vector Backbone Description: Backbone Marker:ATCC 10326362; Backbone Size:4608; Vector Backbone:pENTR1A-Gent; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:16945906
Comments: shRNA expression; miR30-based topology; TRE Pmin promoter; Entry vector backbone; +ccdB in parent; Multiple unique sites d/s of TRE
Proper citation: RRID:Addgene_25748 Copy
Species: bacteria
Genetic Insert: Promotorless gfp reporter gene
Vector Backbone Description: Vector Backbone:pVS1/p15a; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:11059491
Comments: Please refer to the attached table for a complete list of restriction sites in the MCS.
This plasmid has the XbaI site.
Proper citation: RRID:Addgene_37814 Copy
Species: jellyfish
Genetic Insert: gfpmut 3.1
Vector Backbone Description: Backbone Size:4768; Vector Backbone:pBBRMCS5; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:22820336
Comments: GenBank accession number: JQ895027
pLMB509 contains GFPmut3.1 reporter gene downstream of the inducible promoter tauAp and a ribosomal binding site (RBS), followed by a six-His tag and a transcriptional stop codon. NdeI restriction sites flank the reporter gene, enabling the easy removal of gfpmut3.1 and subsequent replacement with the desired gene in frame.
This plasmid is compatible with BD In-Fusion cloning (Clontech) for the cloning of genes containing NdeI restriction sites
Proper citation: RRID:Addgene_40084 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Gm cassette (BssHII+SphI)
Vector Backbone Description: Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:18353436
Comments: NS1 vector
Gm resistant version of pAM1303.
Both pAM1303 and pAM3511 were digested with BssHII and SphI, and then mix-ligated. The accC1gene (Gm) replaces the aadA gene (SpSm) in the Ω cassette. The upstream T4 terminator was lost due to SphI digestion.
Proper citation: RRID:Addgene_40252 Copy
Species: Synthetic
Genetic Insert: Citrine-NLS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3374; Vector Backbone:pDONR207; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:23421791
Proper citation: RRID:Addgene_44586 Copy
Genetic Insert: Gentamycin
Vector Backbone Description: Backbone Marker:Bagdasarian Lab; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:10447882
Comments: This is a vector control for IPTG-indicible E. coli relA gene.
Proper citation: RRID:Addgene_45475 Copy
Genetic Insert: relA
Vector Backbone Description: Backbone Marker:Bagdasarian Lab; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:10447882
Comments: This is a vector control for IPTG-indicible E. coli relA gene.
Proper citation: RRID:Addgene_45474 Copy
Species: Homo sapiens
Genetic Insert: MEK5-DD
Vector Backbone Description: Backbone Marker:Addgene #25752; Backbone Size:4422; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:23332156
Proper citation: RRID:Addgene_47547 Copy
Species: Synthetic
Genetic Insert: C-D dummy
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Comments: L1 dummy.
Proper citation: RRID:Addgene_54343 Copy
Genetic Insert: cauliflower mosaic virus 35S promoter
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54409 Copy
Genetic Insert: L. japonicus polyubiquitin promoter
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54408 Copy
Genetic Insert: cauliflower mosaic virus 35S promoter
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54406 Copy
Species: Synthetic
Genetic Insert: neomycin phosphotransferase II (nptII)
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54405 Copy
Species: Synthetic
Genetic Insert: 16 aa GS linker + T-Sapphire
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54412 Copy
Species: Synthetic
Genetic Insert: 16 aa GS linker + YFP Venus
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54411 Copy
Species: Synthetic
Genetic Insert: CFP Cerulean
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54417 Copy
Species: Synthetic
Genetic Insert: T-saphhire + 16 aa GS linker
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54424 Copy
Species: Synthetic
Genetic Insert: NES of heat stable inhibitor (PKI)
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54429 Copy
Species: Synthetic
Genetic Insert: NLS of SV40 large T antigen
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54428 Copy
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