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Species: synthetic gene
Genetic Insert: P#38-gfp
Vector Backbone Description: Backbone Size:6055; Vector Backbone:pTE-0X1-GCT5; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Comments: pTE-mcs derivative (Addgene plasmid# 20320) with GFP under the control of a strong promoter
Proper citation: RRID:Addgene_27051 Copy
Genetic Insert: tetR(sB), clpXtb
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6181; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_27373 Copy
Genetic Insert: n/a
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pSMT3; Vector Types:Shuttle vector for mycobacteria; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Comments: Creation and characterisation of a high-copy-number version of the pAL5000 mycobacterial replicon. Tuberculosis (Edinb) 87: 481–488.
There are known discrepancies between the Addgene QC sequence and the depositor's full sequence--these are likely due to how the full sequence was assembled and should not affect function.
Proper citation: RRID:Addgene_26589 Copy
Species: synthetic gene derived from Tn10 tetR
Genetic Insert: Psmyc-tetR#28
Vector Backbone Description: Backbone Size:5133; Vector Backbone:pTE-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Comments: There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.
Proper citation: RRID:Addgene_20319 Copy
Vector Backbone Description: Backbone Marker:Ghader Bashiri; Backbone Size:4921; Vector Backbone:pYUB1049; Vector Types:Bacterial Expression, mycobacteria shuttle vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21209917
Comments: Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details.
Proper citation: RRID:Addgene_37277 Copy
Species: Other
Genetic Insert: gRNA1(gsdA)
Vector Backbone Description: Backbone Marker:Michael Sauer (Sarkari et al. 2017) Addgene #90278; Vector Backbone:pMST665_BB3_L 23_gRNAempty_cas9_BbsI; Vector Types:Bacterial Expression, CRISPR, A. niger; Bacterial Resistance:Hygromycin
Proper citation: RRID:Addgene_222719 Copy
Species: Homo sapiens
Genetic Insert: HRASG12V
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:40737085
Proper citation: RRID:Addgene_242639 Copy
Vector Backbone Description: Backbone Marker:Matthew Bennett; Backbone Size:5611; Vector Backbone:pYUBcLIC-GFP; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:35750296
Comments: pYUB vector backbone originates from pYUB28b (Addgene plasmid # 37277 ; http://n2t.net/addgene:37277 ; RRID:Addgene_37277).
Reference: Metabolic engineering of cofactor F420 production in Mycobacterium smegmatis. Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN. PLoS One. 2010 Dec 29;5(12):e15803. doi: 10.1371/journal.pone.0015803 PubMed 21209917
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LIC cassette together with folding reporter GFP and purification tag sequence originate from pREcLIC-GFP.
Reference: Geertsma, E., Poolman, B. High-throughput cloning and expression in recalcitrant bacteria. Nat Methods 4, 705–707 (2007). doi: 10.1038/nmeth1073 PubMed 17643108
Proper citation: RRID:Addgene_183509 Copy
Genetic Insert: this strain is a P2 lysogen derived from E. coli EMG C5545 strain
Vector Backbone Description: Vector Backbone:This is a strain.; Vector Types:; Bacterial Resistance:Hygromycin
Defining Citation: PMID:36731126
Proper citation: RRID:Addgene_197132 Copy
Species: Synthetic
Genetic Insert: attR
Vector Backbone Description: Backbone Marker:Aubry C. et al. Appl Environ Mic 2019. Modular and Integrative Vectors for Synthetic Biology Applications in Streptomyces spp; Backbone Size:5835; Vector Backbone:pOSV805; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:35456877
Proper citation: RRID:Addgene_172194 Copy
Species: Synthetic
Genetic Insert: mCherry-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_186349 Copy
Species: Synthetic
Genetic Insert: mCherry-LaminB1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_186348 Copy
Species: Synthetic
Genetic Insert: EBFP2-LaminB1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184460 Copy
Species: Synthetic
Genetic Insert: iRFP702-3xNLS
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184454 Copy
Species: Synthetic
Genetic Insert: mCardinal-LaminB1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184452 Copy
Species: Synthetic
Genetic Insert: mCardinal-3xNLS
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184450 Copy
Species: Synthetic
Genetic Insert: mCardinal-CAAX
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184451 Copy
Species: Synthetic
Genetic Insert: EBFP2-3xNLS
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184458 Copy
Species: Synthetic
Genetic Insert: EBFP2-CAAX
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184459 Copy
Vector Backbone Description: Backbone Size:3295; Vector Backbone:BB3; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29221460
Proper citation: RRID:Addgene_98543 Copy
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