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Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71503 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71504 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71502 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Proper citation: RRID:Addgene_71507 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Proper citation: RRID:Addgene_71508 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.10; Vector Types:Mammalian Expression, humanSTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: Note: The plasmid for enhancer-activity testing by luciferase assays has been used in the Arnold et al. 2013 publication. It should be replaced for all enhancer activity tests mammalian cells with the updated version reported by Muerdter et al. 2017. The improved version is Addgene plasmid # 99297. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017).
Gateway-cassette was added between the KpnI and BglII sites and SCP1 between BglII and HindIII
Proper citation: RRID:Addgene_71510 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBlueScript SK(+); Vector Types:Gateway entry vector for use in multisite Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21343429
Proper citation: RRID:Addgene_71744 Copy
Species: Synthetic
Genetic Insert: attR1/attR2 cassette
Vector Backbone Description: Vector Backbone:pTol2005b; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73484 Copy
Species: Synthetic
Genetic Insert: lmo2 promoter:mCherry-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73489 Copy
Species: Synthetic
Genetic Insert: lmo2 promoter:EGFP-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73488 Copy
Species: Synthetic
Genetic Insert: fli1ep:6xMyc-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73490 Copy
Species: Synthetic
Genetic Insert: fli1ep:mCherry-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73493 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5211; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27405979
Proper citation: RRID:Addgene_73638 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6987; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27405979
Proper citation: RRID:Addgene_73636 Copy
Vector Backbone Description: Backbone Size:4181; Vector Backbone:PCS2+ was modified to generate pCSf107mT; Vector Types:Mammalian Expression, in vitro transcription or translation off of an SP6 promoter, Xenopus expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26391338
Proper citation: RRID:Addgene_67618 Copy
Vector Backbone Description: Backbone Size:4181; Vector Backbone:PCS2+ was modified to generate pCSf107mT; Vector Types:Mammalian Expression, in vitro transcription or translation off of an SP6 promoter, Xenopus expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26391338
Proper citation: RRID:Addgene_67619 Copy
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_67711 Copy
Vector Backbone Description: Vector Backbone:pmTol2; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27578780
Proper citation: RRID:Addgene_67745 Copy
Vector Backbone Description: Vector Backbone:pWB.attB; Vector Types:Zebrafish transgenesis; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23723152
Proper citation: RRID:Addgene_68313 Copy
Vector Backbone Description: Backbone Marker:Mosimann lab, Addgene plasmid #68313; Vector Backbone:pDestattB; Vector Types:Zebrafish transgenesis; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23723152
Proper citation: RRID:Addgene_68341 Copy
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