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http://www.addgene.org/158721

Species: Other
Genetic Insert: Sth1 sgRNA scaffold
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31992616
Comments: insert sizes: 171 bp and 3366 bp

Proper citation: RRID:Addgene_158721 Copy   

•   Source: Addgene

http://www.addgene.org/172356

Genetic Insert: scbR
Vector Backbone Description: Backbone Size:5715; Vector Backbone:pSET152; Vector Types:Bacterial Expression, Synthetic Biology, Integrative into Streptomyces genome via serine integrase (like parent pSET152); Bacterial Resistance:Hygromycin
Defining Citation: PMID:29130169
Comments: Biarnes-Carrera, Marc, Chang Kwon Lee, Takuya Nihira, Rainer Breitling, and Eriko Takano. ‘Orthogonal Regulatory Circuits for Escherichia Coli Based on the γ-Butyrolactone System of Streptomyces Coelicolor’. ACS Synthetic Biology 7, no. 4 (2018): 1043–55. https://doi.org/10.1021/acssynbio.7b00425. pTE1062 is replicative in E. coli via pMB1 type origin, and carrying oriT (RP4) for delivery to Streptomyces by conjugation. In Streptomyces, pTE1062 is not replicative, and therefore selection is on integration into the genome mediated by phiC31 integrase.

Proper citation: RRID:Addgene_172356 Copy   

•   Source: Addgene

http://www.addgene.org/17972

Genetic Insert: pmyc1tetO
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: pmyc1tetO is a tet operator containing promoter for mycobacteria. Also see Pubmed IDs 18059281 and 15687379. Derivative of pMS2 containing Pmyc1tetO. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.

Proper citation: RRID:Addgene_17972 Copy   

•   Source: Addgene

http://www.addgene.org/30174

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Wasabi. The Wasabi ORF is present at bp# 39-749.

Proper citation: RRID:Addgene_30174 Copy   

•   Source: Addgene

http://www.addgene.org/30176

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Teal. The Teal ORF is present at bp# 103-813.

Proper citation: RRID:Addgene_30176 Copy   

•   Source: Addgene

http://www.addgene.org/30178

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Crimson. The Crimson ORF is present at bp# 104-781.

Proper citation: RRID:Addgene_30178 Copy   

•   Source: Addgene

http://www.addgene.org/30177

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with EBFP2. The EBFP2 ORF is present at bp# 104-823.

Proper citation: RRID:Addgene_30177 Copy   

•   Source: Addgene

http://www.addgene.org/30170

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Addgene Plasmid 30171; Backbone Size:0; Vector Backbone:pMSP12::dsRed2; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Comments: This plasmid was derived from pMSP12::dsRed2 by interupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp#4947 when compared to the full sequence information provided by the depositing laboratory. This mismatch is not a concern for the function of the plasmid.

Proper citation: RRID:Addgene_30170 Copy   

•   Source: Addgene

http://www.addgene.org/126601

Vector Backbone Description: Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189

Proper citation: RRID:Addgene_126601 Copy   

•   Source: Addgene

http://www.addgene.org/126600

Vector Backbone Description: Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189

Proper citation: RRID:Addgene_126600 Copy   

•   Source: Addgene

http://www.addgene.org/126606

Vector Backbone Description: Backbone Size:5903; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189

Proper citation: RRID:Addgene_126606 Copy   

•   Source: Addgene

http://www.addgene.org/134316

Species: Synthetic
Genetic Insert: hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31672972
Comments: pSiMPlh_N should be cotransformed with pSiMPlh_C (or vice versa) in E. Coli to get a reconstituted functional HPT thereby conferring resistance against hygromycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Please see the Sequences link for the individual plasmid sequences. The sample from Addgene will have both the plasmids pSiMPlh_N + pSiMPlh_C in it for the interested antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.

Proper citation: RRID:Addgene_134316 Copy   

•   Source: Addgene

http://www.addgene.org/110093

Vector Backbone Description: Vector Backbone:pCOND; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571

Proper citation: RRID:Addgene_110093 Copy   

•   Source: Addgene

http://www.addgene.org/110087

Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571

Proper citation: RRID:Addgene_110087 Copy   

•   Source: Addgene

http://www.addgene.org/110084

Vector Backbone Description: Vector Backbone:pACEC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571

Proper citation: RRID:Addgene_110084 Copy   

•   Source: Addgene

http://www.addgene.org/110090

Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571

Proper citation: RRID:Addgene_110090 Copy   

•   Source: Addgene

http://www.addgene.org/104625

Species: Other
Genetic Insert: FnCpf1
Vector Backbone Description: Vector Backbone:pMV261; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30039929
Comments: The other inserted are "crRNA to ligD" and "hygromysin resistance gene". "crRNA to ligD" species is synthetic, promoter is Pj23119. Shuttle vector Mycobacterium smegmatis/E.coli

Proper citation: RRID:Addgene_104625 Copy   

•   Source: Addgene

http://www.addgene.org/249819

Vector Backbone Description: Backbone Marker:Addgene 25012; Backbone Size:6000; Vector Backbone:pKW-08; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:27114277

Proper citation: RRID:Addgene_249819 Copy   

•   Source: Addgene

http://www.addgene.org/249818

Species: Mycobacterium tuberculosis
Genetic Insert: Mycobacterium tuberculosis topoisomerase I
Vector Backbone Description: Backbone Marker:Addgene 25012; Backbone Size:6000; Vector Backbone:pKW-08; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:27114277

Proper citation: RRID:Addgene_249818 Copy   

•   Source: Addgene

http://www.addgene.org/252057

Vector Backbone Description: Backbone Size:5888; Vector Backbone:pKEK2201; Vector Types:Sucrose counter-selection allelic exchange vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:39566662

Proper citation: RRID:Addgene_252057 Copy   

•   Source: Addgene