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Species: Homo sapiens
Genetic Insert: CHMP2A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLNCX2; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29972351
Proper citation: RRID:Addgene_115330 Copy
Species: Homo sapiens
Genetic Insert: PARK7E163K
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2790; Vector Backbone:pENTR223; Vector Types:gateway cloning into appropriate destination vectors; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31536960
Comments: Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation.
Proper citation: RRID:Addgene_115181 Copy
Species: Homo sapiens
Genetic Insert: CR (CRISPR/Cas9-resistant)-PDHA2S291A
Vector Backbone Description: Backbone Marker:David Root, Addgene plasmid # 41392; Backbone Size:8270; Vector Backbone:pLEX-307; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31536960
Proper citation: RRID:Addgene_115203 Copy
Species: Homo sapiens
Genetic Insert: CR (CRISPR/Cas9-resistant)-PDHA2S293A
Vector Backbone Description: Backbone Marker:David Root, Addgene plasmid # 41392; Backbone Size:8270; Vector Backbone:pLEX-307; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31536960
Proper citation: RRID:Addgene_115204 Copy
Species: Homo sapiens
Genetic Insert: CHMP1B
Vector Backbone Description: Vector Backbone:pCA528; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32251413
Proper citation: RRID:Addgene_115325 Copy
Species: Homo sapiens
Genetic Insert: SOX10
Vector Backbone Description: Backbone Size:9100; Vector Backbone:pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2 (AddGene 112668); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29337119
Proper citation: RRID:Addgene_115240 Copy
Species: Homo sapiens
Genetic Insert: Rac1 5'-UTR
Vector Backbone Description: Vector Backbone:pFL-SV40; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30373933
Proper citation: RRID:Addgene_115354 Copy
Species: Homo sapiens
Genetic Insert: Rab7a fused to mEmerald
Vector Backbone Description: Backbone Marker:Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24; Backbone Size:5235; Vector Backbone:pAAV-SIBR-anti_I; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_115238 Copy
Species: Homo sapiens
Genetic Insert: 4xStat6 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4267; Vector Backbone:pGL4; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: This plasmid was generated by replacing the HIndIII(blunt)-AdeI fragment of p2xSTAT6-Luc2P (Addgene plasmid 35486) with the KpnI(blunt)-AdeI fragment of p2xSTAT6-Luc2P. The promoter insert consists of four tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG193.
Proper citation: RRID:Addgene_35554 Copy
Species: Homo sapiens
Genetic Insert: RARalpha
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15657432
Proper citation: RRID:Addgene_35557 Copy
Species: Homo sapiens
Genetic Insert: RARalpha
Vector Backbone Description: Vector Backbone:pcDNA 6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15657432
Proper citation: RRID:Addgene_35556 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Comments: Please note that Addgene's sequencing results identified an insert at bp# 25/26 and a single nucleotide mismatch at bp# 38 when compared to the full plasmid sequence provided by the depositing laboratory. The insert at 25/26 and mismatch at 38 was due to an error made in assembling the promoter and plasmid sequences. Addgene's sequencing results are correct at these locations.
Proper citation: RRID:Addgene_35542 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Comments: Please note that Addgene's sequencing results identified a deletion at bp# 25 & 26 and a single nucleotide mismatch at bp# 47 when compared to the full plasmid sequence provided by the depositing laboratory. The deletion at 25&26 was due to an error made in assembly of sequence when combining promoter with plasmid sequence. The mismatch at bp#47 is not known to affect plasmid function.
Proper citation: RRID:Addgene_35541 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Proper citation: RRID:Addgene_35546 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Comments: Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp# 210 when compared to the full plasmid sequence provided by the depositing laboratory. This mismatch is not known to affect the function of the plasmid.
Proper citation: RRID:Addgene_35540 Copy
Species: Homo sapiens
Genetic Insert: 3'UTR of ZEB1
Vector Backbone Description: Backbone Marker:Witold Filipowicz; Backbone Size:6380; Vector Backbone:pCI-neo-RL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18376396
Comments: Mutations in the miR-200b seed regions of the ZEB1 3'UTR were generated using the QuikChange Multi site-directed mutagenesis kit.
The ZEB1 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz.
Proper citation: RRID:Addgene_35537 Copy
Species: Homo sapiens
Genetic Insert: 3'UTR of ZEB2 gene
Vector Backbone Description: Backbone Marker:Witold Filipowicz; Backbone Size:6380; Vector Backbone:pCI-neo-RL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18376396
Comments: The ZEB2 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz.
Pillai R, Bhattacharyya S, Artus C, Zoller T, Cougot N, Basyuk E, Bertrand E, Filipowicz W (2005) Inhibition of translational initiation by let-7 microRNA in human cells.
Science 309:1573-1576
Please note that Addgene's sequencing results identified a single nucleotide deletion at bp# 2440 when compared to the full plasmid sequence provided by the depositing laboratory. Addgene's sequencing results are correct as the extra base in the sequence from the depositing laboratory was an error due to a sequencing artifact.
Proper citation: RRID:Addgene_35536 Copy
Species: Homo sapiens
Genetic Insert: miR200c-141 genomic cluster
Vector Backbone Description: Backbone Marker:Scott Hammond; Backbone Size:8749; Vector Backbone:pLenti 4.1Ex; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18376396
Proper citation: RRID:Addgene_35534 Copy
Species: Homo sapiens
Genetic Insert: KRAS(G12V)
Vector Backbone Description: Backbone Marker:Malcom Moore; Vector Backbone:pUltra; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22318865
Proper citation: RRID:Addgene_35493 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Comments: Please note that Addgene's sequencing results identified a few nucleotide mismatches at bp# 253, 457, 465, 632, 681 & 1463 when compared to the sequence provided by the depositing laboratory. These differences are not known to affect the function of the plasmid.
Proper citation: RRID:Addgene_35539 Copy
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