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Species: Homo sapiens
Genetic Insert: ATP sensor GRAB_ATP1.0
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5121; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34942116
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.02.24.432680v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167579 Copy
Species: Homo sapiens
Genetic Insert: ATP-insensitive sensor GRAB_ATP1.0mut (control sensor)
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:4495; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34942116
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.02.24.432680v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167578 Copy
Genetic Insert: chloramphenicol acetyl transferase
Vector Backbone Description: Backbone Marker:Jan-Peter van Pijkeren; Backbone Size:9747; Vector Backbone:pVPL3004; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34232061
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.09.01.278044v2 for BioRxiv preprint.
Proper citation: RRID:Addgene_167547 Copy
Genetic Insert: E. faecium Com15 recT under an IPTG inducible promoter, with lacI
Vector Backbone Description: Backbone Marker:Luciano Marraffini; Backbone Size:3729; Vector Backbone:plZ12-Spec; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34232061
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.09.01.278044v2 for BioRxiv preprint.
Proper citation: RRID:Addgene_167546 Copy
Species: Escherichia coli
Genetic Insert: cba-his6
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: The His6 tag is located at the C-terminus of colicin B. The parent plasmid is pKP579 in this collection. Colicin B protein expression can be induced by mitomycin C treatment in a recA+ strain, i.e. not DH5alpha (PMID: 17628143).
Proper citation: RRID:Addgene_167658 Copy
Species: Synthetic
Genetic Insert: EKAREN5
Vector Backbone Description: Backbone Size:3450; Vector Backbone:miniTol2 expression vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33795873
Comments: We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis. The generation of stable cell lines using this plasmid requires the co-transfection of a transposase-expressing plasmid. The depositing lab recommends the use of Plasmid 158774.
Proper citation: RRID:Addgene_167818 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:34717688
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167673 Copy
Species: Homo sapiens
Genetic Insert: STA5A
Vector Backbone Description: Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_167799 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:34717688
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167672 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:34717688
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167671 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene Plasmid #52961); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33622708
Proper citation: RRID:Addgene_167929 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene Plasmid #52961); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33622708
Proper citation: RRID:Addgene_167930 Copy
Species: Synthetic
Genetic Insert: dxCas9(3.7)-VPR
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: dxCas9(3.7)-VPR is cloned from dxCas(3.7)-VPR (Addgene Plasmid #108383).
Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167937 Copy
Species: Synthetic
Genetic Insert: KRAB-dCas9-DHFR
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: KRAB-dCas9 cloned from pAAVS1-NDi-CRISPRi (Gen1) (Addgene Plasmid #73497).
Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167935 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-GFP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8939578
Comments: Transcription: linearize with Not I and transcribe with SP6.
Plasmid History:
Constructed by Randall T. Moon.
Notes for Use:
Xdsh of S. Sokol with GFP of Heim et al., Nature 1995, cloned at the carboxy terminal end, followed by a translation stop codon.
Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996.
Addgene QC found 2 point mutations, T511A and T717S, that do not affect function.
Proper citation: RRID:Addgene_16788 Copy
Species: Synthetic
Genetic Insert: EKAREN4(TA)
Vector Backbone Description: Vector Backbone:pLV lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33795873
Comments: We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis.
Proper citation: RRID:Addgene_167830 Copy
Species: Homo sapiens
Genetic Insert: TETv4 (TET1CD-XTEN80-dCas9)
Vector Backbone Description: Backbone Size:5574; Vector Backbone:CAG expression plasmid; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33838111
Proper citation: RRID:Addgene_167983 Copy
Species: Homo sapiens
Genetic Insert: CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
Vector Backbone Description: Backbone Size:4836; Vector Backbone:CAG expression plasmid; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33838111
Proper citation: RRID:Addgene_167981 Copy
Species: Homo sapiens
Genetic Insert: TTF1
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167942 Copy
Species: Peltigera membranacea cyanobiont 210A
Genetic Insert: PmcCAST minimal CRISPR array
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168154 Copy
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