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Species: Other
Genetic Insert: S718 term
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235439 Copy
Species: Synthetic
Genetic Insert: mEBSn-mini35S (A1-B1)
Vector Backbone Description: Vector Backbone:pUPD2; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40976865
Proper citation: RRID:Addgene_254063 Copy
Species: E. coli
Genetic Insert: A1-amtR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2cd34 parts are expression parts in the CTK system. They just need promoters to be fully functional
Proper citation: RRID:Addgene_248497 Copy
Species: E. coli
Genetic Insert: B1-bm3R1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2cd34 parts are expression parts in the CTK system. They just need promoters to be fully functional
Proper citation: RRID:Addgene_248498 Copy
Species: E. coli
Genetic Insert: B2-bm3R1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2cd34 parts are expression parts in the CTK system. They just need promoters to be fully functional
Proper citation: RRID:Addgene_248499 Copy
Species: E. coli
Genetic Insert: L3S2P55
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 4 pasts are terminators in the CTK system
Proper citation: RRID:Addgene_248471 Copy
Vector Backbone Description: Backbone Size:4047; Vector Backbone:pSpy3C; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white.
Resource Information: To create pSpy3C, we took the minimal pWV01* replicon from pBAV1K-T5-gfp (Plasmid #26702), the chloramphenicol resistance cassette from pIB166 (Plasmid #90189) and the MCS featuring the Plac_mrfp cassette from pBS3Clux (#55172).
Please download the file linked below to obtain a well annotated map of the plasmid.
Proper citation: RRID:Addgene_220253 Copy
Genetic Insert: sgRNA - cas9
Vector Backbone Description: Backbone Size:6603; Vector Backbone:pNORg10; Vector Types:E. coli - R. gnavus shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41820342
Comments: Please visit https://doi.org/10.1101/2025.08.12.669022 for bioRxiv preprint.
Proper citation: RRID:Addgene_249345 Copy
Species: E. coli
Genetic Insert: RiboJ
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2c parts are insulating ribozymes in the CTK system
Proper citation: RRID:Addgene_248412 Copy
Species: E. coli
Genetic Insert: L3S3P31
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 4 pasts are terminators in the CTK system
Proper citation: RRID:Addgene_248473 Copy
Species: E. coli
Genetic Insert: J23116
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2ab parts are single prmoters in the CTK system
Proper citation: RRID:Addgene_248405 Copy
Species: Synthetic
Genetic Insert: ALFA-Fibcon
Vector Backbone Description: Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://doi.org/10.1101/2024.03.12.584464 for bioRxiv preprint.
Proper citation: RRID:Addgene_236354 Copy
Species: Synthetic
Genetic Insert: GAL4-VP64-ERT2
Vector Backbone Description: Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://doi.org/10.1101/2024.03.12.584464 for bioRxiv preprint.
Proper citation: RRID:Addgene_236358 Copy
Species: Synthetic
Genetic Insert: VP64-NS3
Vector Backbone Description: Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://doi.org/10.1101/2024.03.12.584464 for bioRxiv preprint.
Proper citation: RRID:Addgene_236359 Copy
Species: E. coli
Genetic Insert: P2-phlF
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2cd34 parts are expression parts in the CTK system. They just need promoters to be fully functional
Proper citation: RRID:Addgene_248508 Copy
Species: E. coli
Genetic Insert: P3-phlF
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2cd34 parts are expression parts in the CTK system. They just need promoters to be fully functional
Proper citation: RRID:Addgene_248509 Copy
Genetic Insert: EcDRT3 (Drt3a + Drt3b + ncRNA)
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41990131
Proper citation: RRID:Addgene_255316 Copy
Species: Synthetic
Genetic Insert: G1PylRSMIFAF
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253672 Copy
https://dgrc.bio.indiana.edu/stock/1233725
Species: Drosophila melanogaster
Genetic Insert: RpS30
Defining Citation: PMID:12176937
Comments: BDGP ESTs
Proper citation: RRID:DGRC_1233725 Copy
https://dgrc.bio.indiana.edu/stock/1232236
Species: Drosophila melanogaster
Genetic Insert: Xbp1
Defining Citation: PMID:12176937
Comments: BDGP ESTs
Proper citation: RRID:DGRC_1232236 Copy
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