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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pAAV-GfaABC1D-GRAB_ATP1.0
 
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RRID:Addgene_167579 ATP sensor GRAB_ATP1.0 Homo sapiens Ampicillin PMID:34942116 Please visit https://www.biorxiv.org/content/10.1101/2021.02.24.432680v1 for bioRxiv preprint. Backbone Marker:Addgene; Backbone Size:5121; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:09:45 5
pAAV-hsyn-GRAB_ATP1.0mut
 
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RRID:Addgene_167578 ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) Homo sapiens Ampicillin PMID:34942116 Please visit https://www.biorxiv.org/content/10.1101/2021.02.24.432680v1 for bioRxiv preprint. Backbone Marker:Addgene; Backbone Size:4495; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:09:45 1
pCas9
 
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RRID:Addgene_167547 chloramphenicol acetyl transferase Chloramphenicol PMID:34232061 Please visit https://www.biorxiv.org/content/10.1101/2020.09.01.278044v2 for BioRxiv preprint. Backbone Marker:Jan-Peter van Pijkeren; Backbone Size:9747; Vector Backbone:pVPL3004; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol 2026-08-15 01:09:44 1
pRecT_2
 
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RRID:Addgene_167546 E. faecium Com15 recT under an IPTG inducible promoter, with lacI Spectinomycin PMID:34232061 Please visit https://www.biorxiv.org/content/10.1101/2020.09.01.278044v2 for BioRxiv preprint. Backbone Marker:Luciano Marraffini; Backbone Size:3729; Vector Backbone:plZ12-Spec; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:45 1
pKP864
 
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1+ mentions
RRID:Addgene_167658 cba-his6 Escherichia coli Chloramphenicol The His6 tag is located at the C-terminus of colicin B. The parent plasmid is pKP579 in this collection. Colicin B protein expression can be induced by mitomycin C treatment in a recA+ strain, i.e. not DH5alpha (PMID: 17628143). Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol cba-his6 2026-08-15 01:09:46 1
miniTol2 x EF1a_EKAREN5 + PGK-blast
 
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RRID:Addgene_167818 EKAREN5 Synthetic Ampicillin PMID:33795873 We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis. The generation of stable cell lines using this plasmid requires the co-transfection of a transposase-expressing plasmid. The depositing lab recommends the use of Plasmid 158774. Backbone Size:3450; Vector Backbone:miniTol2 expression vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin K424P, K426W 2026-08-15 01:09:47 1
pSF278
 
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RRID:Addgene_167673 attL1-ccdB-attL2 Escherichia coli Chloramphenicol and Kanamycin PMID:34717688 Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint. Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin 2026-08-15 01:09:46 1
MAC_N_STA5A
 
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RRID:Addgene_167799 STA5A Homo sapiens Ampicillin PMID:35140242 Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin no mutations 2026-08-15 01:09:47 1
pSF464
 
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1+ mentions
RRID:Addgene_167672 attL1-ccdB-attL2 Escherichia coli Chloramphenicol and Kanamycin PMID:34717688 Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint. Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin 2026-08-15 01:09:46 1
pSF463
 
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1+ mentions
RRID:Addgene_167671 attL1-ccdB-attL2 Escherichia coli Chloramphenicol and Kanamycin PMID:34717688 Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint. Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin 2026-08-15 01:09:46 1
LentiGuide-TagBFP-2A-BSD
 
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1+ mentions
RRID:Addgene_167929 Ampicillin PMID:33622708 Backbone Marker:Feng Zhang (Addgene Plasmid #52961); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:48 1
LentiGuide-TagRFP-2A-BSD
 
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RRID:Addgene_167930 Ampicillin PMID:33622708 Backbone Marker:Feng Zhang (Addgene Plasmid #52961); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:48 1
pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G
 
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RRID:Addgene_167937 dxCas9(3.7)-VPR Synthetic Ampicillin PMID:34612202 dxCas9(3.7)-VPR is cloned from dxCas(3.7)-VPR (Addgene Plasmid #108383). Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint. Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:09:48 1
pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G
 
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RRID:Addgene_167935 KRAB-dCas9-DHFR Synthetic Ampicillin PMID:34612202 KRAB-dCas9 cloned from pAAVS1-NDi-CRISPRi (Gen1) (Addgene Plasmid #73497). Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint. Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:09:48 6
XE43 Xdsh-GFP
 
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RRID:Addgene_16788 Xdsh-GFP Xenopus laevis Ampicillin PMID:8939578 Transcription: linearize with Not I and transcribe with SP6. Plasmid History: Constructed by Randall T. Moon. Notes for Use: Xdsh of S. Sokol with GFP of Heim et al., Nature 1995, cloned at the carboxy terminal end, followed by a translation stop codon. Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996. Addgene QC found 2 point mutations, T511A and T717S, that do not affect function. Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:48 3
pLV x EF1a_EKAREN4(TA)-ires-puro
 
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RRID:Addgene_167830 EKAREN4(TA) Synthetic Ampicillin PMID:33795873 We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis. Vector Backbone:pLV lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin K424P, K426W, T420A 2026-08-15 01:09:47 1
TETv4
 
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RRID:Addgene_167983 TETv4 (TET1CD-XTEN80-dCas9) Homo sapiens Ampicillin PMID:33838111 Backbone Size:5574; Vector Backbone:CAG expression plasmid; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:09:49 8
CRISPRoff-v2.1
 
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10+ mentions
RRID:Addgene_167981 CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB) Homo sapiens Ampicillin PMID:33838111 Backbone Size:4836; Vector Backbone:CAG expression plasmid; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:49 20
pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G
 
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RRID:Addgene_167942 TTF1 Homo sapiens Ampicillin PMID:34612202 Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint. Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:09:48 2
pMS21: pHelper(PmcCAST)_entry
 
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RRID:Addgene_168154 PmcCAST minimal CRISPR array Peltigera membranacea cyanobiont 210A Spectinomycin PMID:33770501 Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:51 1

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