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Species: Saccharomyces cerevisiae
Genetic Insert: SWA2
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253001 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SRP54
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253004 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MNS1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253005 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CWH41
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253007 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Aga2
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252964 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Aga2
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252965 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Aga2
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252967 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SED5
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253010 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pir
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252961 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pir
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252963 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Aga2 mRuby2
Vector Backbone Description: Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253043 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AGA1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253023 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AGA1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253024 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mRuby2-Aga2
Vector Backbone Description: Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253033 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Srs2-C-terminus
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_247774 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUMO family protein SMT3
Vector Backbone Description: Backbone Size:5228; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41927759
Comments: This plasmid contains a SUMO protein construct to serve as a control protein for binding experiments using the AH-SUMO platform, to verify non-interaction between the SUMO domain and liposomes of choice. It contains the SUMO1-98 region of the SMT3_yeast gene, followed by a Cysteine, a TEV site and a hexa-histidine site. On the N-terminal of the SUMO domain, it contains a region encoding for MGSGS, which is there to mimic the structure of a AH-SUMO platform that contains an N-terminal peptide of interest , serving as a better control.
Please visit https://doi.org/10.1101/2025.05.12.653516 for the bioRxiv preprint.
Proper citation: RRID:Addgene_254139 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Leaf compost cutinase
Vector Backbone Description: Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253050 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET15
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253016 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AGA1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253029 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4 DNA binding domain
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5360; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Proper citation: RRID:Addgene_175949 Copy
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