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Species: Synthetic
Genetic Insert: G-CEPIA1er
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4819; Vector Backbone:pCMV/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24923787
Proper citation: RRID:Addgene_58215 Copy
Species: Synthetic
Genetic Insert: CEPIA2mt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4799; Vector Backbone:pCMV/myc/mito; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24923787
Proper citation: RRID:Addgene_58218 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: ECFP/Venus mitochondrial-targeted calcium biosensor. Excitation = 433 / 515 ; Emission = 475 / 528
Proper citation: RRID:Addgene_58183 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: ECFP/Venus cytosolic calcium biosensor. Excitation = 433 / 515; Emission = 475 / 528
Proper citation: RRID:Addgene_58182 Copy
Species: Synthetic
Genetic Insert: CEPIA4mt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4799; Vector Backbone:pCMV/myc/mito; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24923787
Proper citation: RRID:Addgene_58220 Copy
Species: Mus musculus
Genetic Insert: Plexin A1
Vector Backbone Description: Vector Backbone:pBS KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22723296
Comments: cDNA from expressed sequence tag (EST) IMAGE clones (Invitrogen), clone ID 1853022 (Plxna1).
For antisense RNA transcription, use EcoR1 and T7.
Proper citation: RRID:Addgene_58237 Copy
Species: Homo sapiens
Genetic Insert: PITP alpha
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:4671; Vector Backbone:pET14b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14962392
Proper citation: RRID:Addgene_58258 Copy
Genetic Insert: Enhanced Green Fluorescence Protein, Puromycin resistance gene
Vector Backbone Description: Backbone Size:4941; Vector Backbone:HSC1 retroviral vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23752310
Comments: Please note that there is a small deletion (32bp) just after the 3’LTR inside the cloning vector that removes the M13R primer region. The depositing lab was able to produce viruses with good titer and doesn’t think the missing 32bp is a major concern.
Proper citation: RRID:Addgene_58254 Copy
Species: Rattus norvegicus
Genetic Insert: Nrxn2beta AS4(-)
Vector Backbone Description: Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / Bsu36I).
Insert can also be released by single digest using XhoI.
The rat neurexin 2 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58268 Copy
Species: Rattus norvegicus
Genetic Insert: Nrxn1beta AS4(-)
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / KpnI).
Insert can also be released by single digest using XhoI.
The rat neurexin 1 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the region homologous to alternatively spliced segment 5 (AS5) of mouse neurexin 1 beta (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58267 Copy
Species: Homo sapiens
Genetic Insert: Neuroserpin
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24581498
Proper citation: RRID:Addgene_58260 Copy
Species: Homo sapiens
Genetic Insert: FADD
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8519; Vector Backbone:pLVX-IRES-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24581498
Proper citation: RRID:Addgene_58263 Copy
Species: Rattus norvegicus
Genetic Insert: Nrxn1alpha
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI).
Insert can also be released without HA-tag (e.g. by SpeI / XmaI digest).
The rat neurexin 1 alpha encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the regions homologous to alternatively spliced segment 3 and 5 (AS3 and AS5, respectively) of mouse neurexin 1 alpha (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58266 Copy
Species: Homo sapiens
Genetic Insert: PITP beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20332109
Comments: Addgene QC sequencing has found some mutations in the insert, which should not effect the function of the plasmid.
Proper citation: RRID:Addgene_58279 Copy
Vector Backbone Description: Vector Backbone:mEos A69T ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: A69T photoconvert with 488nm light. Excitation = 505 / 569; Emission = 516 / 581
Proper citation: RRID:Addgene_58150 Copy
Species: Homo sapiens
Genetic Insert: PITP beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18636990
Proper citation: RRID:Addgene_58271 Copy
Species: Homo sapiens
Genetic Insert: Mis12-FKBPx3
Vector Backbone Description: Vector Backbone:inducible knockdown/cDNA RMCE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24637323
Proper citation: RRID:Addgene_58289 Copy
Species: Drosophila melanogaster
Genetic Insert: E-cadherin tension sensor
Vector Backbone Description: Backbone Marker:Clara Moch at Jean-Rene Huynh's Laboratory; Backbone Size:12396; Vector Backbone:pUWR; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24855950
Proper citation: RRID:Addgene_58325 Copy
Species: S.pyogenes
Genetic Insert: SpCas9
Vector Backbone Description: Vector Backbone:pLKO.005; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24952903
Proper citation: RRID:Addgene_58329 Copy
Species: Drosophila melanogaster
Genetic Insert: Cad-Venus
Vector Backbone Description: Backbone Marker:Clara Moch at Jean-Rene Huynh's Laboratory; Backbone Size:12396; Vector Backbone:pUWR; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24855950
Proper citation: RRID:Addgene_58328 Copy
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