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Species: Danio rerio
Genetic Insert: ZebrafishCommunity-srf-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TGTGCTGCCCACCTGTAG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_47278 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-Stat3-left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TGCAGCAGTTGGAGACGC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_47279 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-snai1b-left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32285; Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TGAAGTTTCGAGGGGATA It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_47271 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-sqstm1-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TCCTTGTTGCAGTCCTCC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_47276 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-srf-left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TGGACGCTCTGGTAATGG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_47277 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-yjefn3-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TCAATCAGCTGCTGCTGG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_47310 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-sqstm1-left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TGTCGATGACAGTGAAAG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_47275 Copy
Species: Danio rerio
Genetic Insert: Alpha B-crystallin
Vector Backbone Description: Vector Backbone:pET-21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Comments: Addgene's full plasmid sequencing results found that the plasmid backbone differs from what is reported in the associated publication, however, the change in backbone is not expected to affect the use of the plasmids to produce recombinant protein.
Proper citation: RRID:Addgene_47665 Copy
Species: Danio rerio
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Proper citation: RRID:Addgene_47630 Copy
Species: Danio rerio
Genetic Insert: acvr1l
Vector Backbone Description: Backbone Size:4163; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33827919
Proper citation: RRID:Addgene_201905 Copy
Species: Danio rerio
Genetic Insert: acvr1l
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pCS2+ or pCS2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33827919
Proper citation: RRID:Addgene_201904 Copy
Species: Danio rerio
Genetic Insert: zCREST3
Vector Backbone Description: Vector Backbone:pDestTol2CG2-U6:gRNA; Vector Types:Tol2 destination vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36384112
Proper citation: RRID:Addgene_199333 Copy
Species: Danio rerio
Genetic Insert: zCREST3
Vector Backbone Description: Vector Backbone:pDestTol2CG2-U6:gRNA; Vector Types:Tol2 destination vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36384112
Proper citation: RRID:Addgene_199332 Copy
Species: Danio rerio
Genetic Insert: LSE_C
Vector Backbone Description: Vector Backbone:pDonP4-P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36384112
Proper citation: RRID:Addgene_199395 Copy
Species: Danio rerio
Genetic Insert: (-3kb)nrg1 type II
Vector Backbone Description: Vector Backbone:pDonP4-P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36384112
Proper citation: RRID:Addgene_199393 Copy
Species: Danio rerio
Genetic Insert: Alk3 (bmpr1aa) kinase domain + VfLOV domain
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33174840
Proper citation: RRID:Addgene_207614 Copy
Species: Danio rerio
Genetic Insert: Alk8 (acvr1l) kinase domain + VfLOV domain
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33174840
Proper citation: RRID:Addgene_207615 Copy
Species: Danio rerio
Genetic Insert: BMPR2a kinase domain and CTD + VfLOV domain
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33174840
Proper citation: RRID:Addgene_207616 Copy
Species: Danio rerio
Genetic Insert: PMT
Vector Backbone Description: Backbone Size:3320; Vector Backbone:pminiTol2; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236989
Proper citation: RRID:Addgene_203794 Copy
Species: Danio rerio
Genetic Insert: crhr2 pTAL scaffold 1B
Vector Backbone Description: Backbone Marker:Addgene plasmid 38142; Backbone Size:4651; Vector Backbone:RCIscript-GoldyTALEN; Vector Types:mRNA transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23000899
Comments: Please note that some of the NN RVDs of this TALEN plasmid, which recognize G nucleotides were actually made with NK RVDs (which also recognize G nucleotides). This alters a single amino acid within the RVD.
Proper citation: RRID:Addgene_44237 Copy
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