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Species: Rattus norvegicus
Genetic Insert: frizzled-2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-2 cDNA, tagged with a single epitope in the third extracellular loop (at an introduced NheI site) was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The untagged part of the insert was published by Wang et al., JBC 271: 4468 (1996). The myc-tagged insert was made by Jeff Brown, and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16812 Copy
Species: Synthetic
Genetic Insert: PAM for AvCAST + AvPSP1
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168146 Copy
Species: Synthetic
Genetic Insert: ymScarletI
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Comments: Contains the linker region at the 5’ end of the FP, to use with the standard F5 forward primer (GGTGACGGTGCTGGTTTA).
Proper citation: RRID:Addgene_168055 Copy
Species: Synthetic
Genetic Insert: Blue light-inducible AraC dimers in Escherichia coli
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33903769
Proper citation: RRID:Addgene_168050 Copy
Species: Homo sapiens
Genetic Insert: GFP-Flag-Nup62-EPEA
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5365; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33686291
Proper citation: RRID:Addgene_168101 Copy
Species: Anabaena variabilis
Genetic Insert: AvCAST minimal CRISPR array
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168137 Copy
Species: Synthetic
Genetic Insert: HygR gene
Vector Backbone Description: Backbone Size:2648; Vector Backbone:pUC19; Vector Types:Synthetic Biology, Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31518064
Proper citation: RRID:Addgene_168129 Copy
Species: Homo sapiens
Genetic Insert: GNAI1, GNB1, GNG2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:Clontech-style N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34516756
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.02.05.429900v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_168120 Copy
Species: Homo sapiens
Genetic Insert: GNAI2, GNB1, GNG2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:Clontech-style N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34516756
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.02.05.429900v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_168121 Copy
Species: Homo sapiens
Genetic Insert: GNAO1, GNB3, GNG9
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:Clontech-style N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34516756
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.02.05.429900v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_168123 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14688793
Comments: This is a fusion protein consisting of rat Frizzled 1 with a carboxy terminal GFP tag.
Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.
Proper citation: RRID:Addgene_16817 Copy
Species: Rattus norvegicus
Genetic Insert: Fz1-ER
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14688793
Comments: This is a fusion protein consisting of a FKBP domain at the amino terminus of rat Frizzled 1.
The expression of the protein is under the control of a CMV promoter. Once expressed the fusion protein enters the endoplasmic reticulum, where it remains trapped (and acts as a dominant negative Frizzled by trapping endogenous Frizzleds) unless one adds proprietary Ariad small molecule reagents that bind to and unfold the FKBP domain, allowing proteolysis and then processing and membrane targeting of the Frizzled 1. Note that the Frizzleds interact in a homo and heteromeric manner.
Notes for Use: Construct is 8251bp, made 9/16/02 by Ajamete, and
also called Fz1-ER/AKA pC4FZ1agg.
Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.
Proper citation: RRID:Addgene_16816 Copy
Vector Backbone Description: Backbone Size:6762; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD136.61, Ligation number L4816.
Proper citation: RRID:Addgene_1682 Copy
Species: Synthetic
Genetic Insert: N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
Vector Backbone Description: Backbone Marker:Joachim Messing; Backbone Size:2712; Vector Backbone:pUC18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30857538
Proper citation: RRID:Addgene_168298 Copy
Species: Homo sapiens
Genetic Insert: CD63
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5520; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27189348
Proper citation: RRID:Addgene_168220 Copy
Species: Setaria italica
Genetic Insert: Si514722593
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168316 Copy
Species: Setaria italica
Genetic Insert: Si514728335
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168317 Copy
Species: Setaria italica
Genetic Insert: Si944233294
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168311 Copy
Species: Oryza sativa
Genetic Insert: OsaHIP26
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168393 Copy
Species: Setaria italica
Genetic Insert: Si514742730
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168319 Copy
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