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Species: Saccharomyces cerevisiae
Genetic Insert: PMA1-FAPoptim
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The coding sequence of PMA1 lacking the stop codon was PCR amplified with primers containing BamHI and SmaI restriction enzyme adaptors. The PCR product was then inserted into the pRS413-TEF1pr-NFAPoptim plasmid using standard cloning approaches.
Proper citation: RRID:Addgene_221122 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPH1-FAPoptim
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The coding sequence of VPH1 lacking the stop codon was PCR amplified with primers containing BamHI and SmaI restriction enzyme adaptors. The PCR product was then inserted into the pRS413-TEF1pr-NFAPoptim plasmid using standard cloning approaches.
Proper citation: RRID:Addgene_221125 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC7-FAPoptim
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The coding sequence of SEC7 lacking the stop codon was PCR amplified with primers containing BamHI and SmaI restriction enzyme adaptors. The PCR product was then inserted into the pRS413-TEF1pr-NFAPoptim plasmid using standard cloning approaches.
Proper citation: RRID:Addgene_221124 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LifeAct
Vector Backbone Description: Backbone Marker:EMD Biosciences; Vector Backbone:pSEMS(26m); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38987559
Comments: StayGold from Addgene #185823
Hirano M, Ando R, Shimozono S, Sugiyama M, Takeda N, Kurokawa H, Deguchi R, Endo K, Haga K, Takai-Todaka R, Inaura S, Matsumura Y, Hama H, Okada Y, Fujiwara T, Morimoto T, Katayama K, Miyawaki A. A highly photostable and bright green fluorescent protein. Nat Biotechnol. 2022 Jul;40(7):1132-1142. doi: 10.1038/s41587-022-01278-2. Epub 2022 Apr 25. Erratum in: Nat Biotechnol. 2022 Sep;40(9):1412. doi: 10.1038/s41587-022-01469-x. PMID: 35468954; PMCID: PMC9287174.
Proper citation: RRID:Addgene_222947 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41794024
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227752 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41794024
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227753 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41794024
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227746 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41794024
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227747 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41794024
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227748 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41794024
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227749 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41794024
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227755 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41794024
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227757 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41794024
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227759 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227770 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSD1
Vector Backbone Description: Backbone Marker:Sikorski and Hieter, 1989, Genetics; Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40037589
Comments: Please visit https://doi.org/10.1101/2024.08.28.610086 for bioRxiv preprint.
Proper citation: RRID:Addgene_226278 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Aga2
Vector Backbone Description: Backbone Size:5740; Vector Backbone:pETcon(-); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38116434
Proper citation: RRID:Addgene_225229 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Aga2
Vector Backbone Description: Backbone Size:5740; Vector Backbone:pETcon(-); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38116434
Proper citation: RRID:Addgene_225228 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YPR201W
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175231 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YCL002C
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175206 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YEL004W
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175208 Copy
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