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Species: Homo sapiens
Genetic Insert: GPC3
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pEF-BOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9628896
Comments: HA tag is after leader sequence. HA tag sequence is - PVYDVPDYA
Proper citation: RRID:Addgene_24670 Copy
Species: Mus musculus
Genetic Insert: Par6
Vector Backbone Description: Backbone Size:10400; Vector Backbone:pMEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15761148
Proper citation: RRID:Addgene_24648 Copy
Species: Homo sapiens
Genetic Insert: Prickle 2
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pGateway 5'CMV Trible Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19379695
Proper citation: RRID:Addgene_24645 Copy
Species: Homo sapiens
Genetic Insert: Prickle 1
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pGateway 5'CMV Trible Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19379695
Proper citation: RRID:Addgene_24644 Copy
Species: Homo sapiens
Genetic Insert: TEAD-4
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMX-Gal4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_24640 Copy
Species: Mus musculus
Genetic Insert: p45 NFE2
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pEF-BOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8078893
Proper citation: RRID:Addgene_24656 Copy
Species: Homo sapiens
Genetic Insert: SGK3
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:5400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16790420
Proper citation: RRID:Addgene_24650 Copy
Species: Mus musculus
Genetic Insert: mFar1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBBR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20111002
Comments: EJ Steen et al. Nature 2009 10.1038/nature08721
Proper citation: RRID:Addgene_24629 Copy
Species: E. coli
Genetic Insert: 'tesA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBBR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20111002
Comments: EJ Steen et al. 2009 Nature 10.1038/nature08721
Proper citation: RRID:Addgene_24625 Copy
Species: Homo sapiens
Genetic Insert: NFATc4
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS.KS+; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10537109
Proper citation: RRID:Addgene_24586 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pADH1 yeCherry::pTEF1 (Ashbya)yeGFP-DHFR
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20017217
Proper citation: RRID:Addgene_24585 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: The CMV promoter from the pLJM1 vector (Addgene) was PCR amplified and cloned into pLD-puro-EcVA using NdeI/MluI to generate pLD-puro-CcVA.
pLD-puro-EcVA (pLD-puromycin resistance- EF1alpha, C-terminal VA tag) was generated as described in the paper from pLKO.1
VA tag= versatile affinity tag, 3XFlag+2XTev+6XHis+2XStrepIII
Proper citation: RRID:Addgene_24588 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20230748
Comments: Mutation reported in:
Clinical, molecular, and genotype-phenotype correlation studies from 25 cases of oral-facial-digital syndrome type 1: a French and Belgian collaborative study.
Thauvin-Robinet C, Cossée M, Cormier-Daire V, Van Maldergem L, Toutain A, Alembik Y, Bieth E, Layet V, Parent P, David A, Goldenberg A, Mortier G, Héron D, Sagot P, Bouvier AM, Huet F, Cusin V, Donzel A, Devys D, Teyssier JR, Faivre L.
J Med Genet. 2006 Jan;43(1):54-61.PMID: 16397067
Proper citation: RRID:Addgene_24581 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20017217
Comments: The TEF1 (S.C.) promoter region has 4 base differences from the published sequences but the authors report that they do not affect transcriptional function.
Proper citation: RRID:Addgene_24584 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20230748
Comments: Mutation reported in:
OFD1, the gene mutated in oral-facial-digital syndrome type 1, is expressed in the metanephros and in human embryonic renal mesenchymal cells.
Romio L, Wright V, Price K, Winyard PJ, Donnai D, Porteous ME, Franco B, Giorgio G, Malcolm S, Woolf AS, Feather SA.
J Am Soc Nephrol. 2003 Mar;14(3):680-9.PMID: 12595504
Proper citation: RRID:Addgene_24580 Copy
Species: Homo sapiens
Genetic Insert: TEAD-4
Vector Backbone Description: Vector Backbone:pRK5-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20123905
Comments: This plasmid is identical to plasmid #33039: pRK5-Myc-TEAD4
Proper citation: RRID:Addgene_24638 Copy
Species: Homo sapiens
Genetic Insert: Yap2
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_24637 Copy
Species: Peptides
Genetic Insert: CB1-CKKp
Vector Backbone Description: Backbone Marker:PMID: 12864922; Backbone Size:0; Vector Backbone:ventx vector; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19472343
Comments: CB1 and the CKKp are the peptides PPPALPPKKR and VKLIPSWTTVILVKSMLRKRSFGNPF, respectively. All the components (Venus, CB1 and CKKp) are linked by an amino acid linker 'AS'.
Proper citation: RRID:Addgene_24597 Copy
Species: E. coli
Genetic Insert: fadD
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBR322; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20111002
Comments: EJ Steen et al. 2009 Nature 10.1038/nature08721
Proper citation: RRID:Addgene_24630 Copy
Species: Peptides
Genetic Insert: IE-LM
Vector Backbone Description: Backbone Marker:PMID: 12864922; Backbone Size:0; Vector Backbone:pCfvtx; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20167369
Comments: IE peptide: RAAITVQRYVRGYDIKEKLCYVALD
LM peptide:
RIRKKLRKNFIAVSAANRFKKI
Proper citation: RRID:Addgene_24596 Copy
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