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Species: Rattus norvegicus
Genetic Insert: gRNA and smFP myc donor
Vector Backbone Description: Vector Backbone:pOC4; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35851300
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.02.474730v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_183438 Copy
Species: Rattus norvegicus
Genetic Insert: SYT1
Vector Backbone Description: Vector Backbone:pGEX-4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32362337
Proper citation: RRID:Addgene_170651 Copy
Species: Rattus norvegicus
Genetic Insert: SYT1
Vector Backbone Description: Vector Backbone:pGEX-4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32362337
Proper citation: RRID:Addgene_170646 Copy
Species: Rattus norvegicus
Genetic Insert: SYT1
Vector Backbone Description: Vector Backbone:pGEX-4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32362337
Proper citation: RRID:Addgene_170644 Copy
Species: Rattus norvegicus
Genetic Insert: SYT1
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32362337
Proper citation: RRID:Addgene_170642 Copy
Species: Rattus norvegicus
Genetic Insert: SYT1
Vector Backbone Description: Vector Backbone:pGEX-4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32362337
Proper citation: RRID:Addgene_170648 Copy
Species: Rattus norvegicus
Genetic Insert: SYT1
Vector Backbone Description: Vector Backbone:pGEX-4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32362337
Proper citation: RRID:Addgene_170649 Copy
Species: Rattus norvegicus
Genetic Insert: TEF2p-Scarlet(rfp)-Link-2xPH
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179074 Copy
Species: Rattus norvegicus
Genetic Insert: TDH3p-Scarlet(rfp)-Link-2xPH
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179073 Copy
Species: Rattus norvegicus
Genetic Insert: TDH3p-Neon(gfp)-Link-2xPH(PLCd)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179072 Copy
Species: Rattus norvegicus
Genetic Insert: Nav1.2
Vector Backbone Description: Backbone Marker:Addgene plasmid 20945; Backbone Size:10093; Vector Backbone:pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27932952
Comments: Please see the linked article - Dumitrescu et al. (2016) - for full details of the cloning procedure used to generate this consruct
Proper citation: RRID:Addgene_91246 Copy
Species: Rattus norvegicus
Genetic Insert: Rattus norvegicus regulator of G-protein signaling 14
Vector Backbone Description: Backbone Marker:Dr. Michel Bouvier (University of Montreal); Vector Backbone:phRLucN2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21880739
Comments: A T95I mutation was found in Rgs14. This does not affect the function of the plasmid.
Proper citation: RRID:Addgene_236397 Copy
Species: Rattus norvegicus
Genetic Insert: Nlgn2
Vector Backbone Description: Vector Backbone:pX601; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42223381
Proper citation: RRID:Addgene_250934 Copy
Species: Rattus norvegicus
Genetic Insert: Nlgn2
Vector Backbone Description: Vector Backbone:pX601; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42223381
Proper citation: RRID:Addgene_250933 Copy
Species: Rattus norvegicus
Genetic Insert: CLIP-170 CAP-Gly
Vector Backbone Description: Vector Backbone:pET15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41662400
Proper citation: RRID:Addgene_255803 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase alpha
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_33330 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21669867
Comments: Please note that, according to NCBI numbering, the mutations are actually S99A, S494A, S510A.
Proper citation: RRID:Addgene_33320 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Proper citation: RRID:Addgene_33326 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Comments: The truncation was made by placing a stop codon at 460, but sequence was not actually removed.
The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_33324 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Proper citation: RRID:Addgene_33329 Copy
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