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Species: Synthetic
Genetic Insert: ChrimsonR-tdTomato
Vector Backbone Description: Backbone Marker:Epoch; Backbone Size:4458; Vector Backbone:AAV-CAG-FLPX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_128589 Copy
Species: Homo sapiens
Genetic Insert: TGFB2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3843; Vector Backbone:pIEx; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235767
Proper citation: RRID:Addgene_128500 Copy
Species: Chlamydomonas noctigama
Genetic Insert: ChrimsonR-GFP
Vector Backbone Description: Backbone Marker:Epoch; Backbone Size:5156; Vector Backbone:AAV- EF1α1.1-FLPX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_128588 Copy
Genetic Insert: Puromycin Resistance
Vector Backbone Description: Vector Backbone:pscALPS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30297740
Proper citation: RRID:Addgene_128504 Copy
Species: Homo sapiens
Genetic Insert: TGFB2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4396; Vector Backbone:pCMV-Sport 6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235767
Proper citation: RRID:Addgene_128503 Copy
Species: Homo sapiens
Genetic Insert: TGFB2 (L21-S414)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4396; Vector Backbone:pCMV-Sport 6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235767
Proper citation: RRID:Addgene_128502 Copy
Species: Homo sapiens
Genetic Insert: RAB5IF
Vector Backbone Description: Backbone Size:10056; Vector Backbone:pPHAGE C-TAP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31536960
Comments: Please note: Plasmid contains a P136L mutation in C20orf24 compared to the NCBI reference squence NP_001186463.1. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_128508 Copy
Genetic Insert: Firefly luciferase
Vector Backbone Description: Backbone Size:8174; Vector Backbone:pPHAGE; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31536960
Proper citation: RRID:Addgene_128507 Copy
Species: Mus musculus
Genetic Insert: Rac1
Vector Backbone Description: Backbone Marker:Addgene plasmid 1765; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31257073
Proper citation: RRID:Addgene_128580 Copy
Species: Homo sapiens
Genetic Insert: Myosin IIB
Vector Backbone Description: Backbone Size:3963; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30540249
Proper citation: RRID:Addgene_128574 Copy
Species: Synthetic
Genetic Insert: Csm1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3419; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:31278272
Proper citation: RRID:Addgene_128572 Copy
Vector Backbone Description: Vector Backbone:FUW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253799
Proper citation: RRID:Addgene_128571 Copy
Species: Homo sapiens
Genetic Insert: 68Q-Httex1 (without proline-rich region) fused to ymsfGFP
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30631438
Proper citation: RRID:Addgene_128560 Copy
Species: Aequorea victoria
Genetic Insert: Venus
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:7470; Vector Backbone:pDEST15; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27455993
Comments: 5' cloning site: AgeI (unknown if destroyed), 3' cloning site: MluI (unknown if destroyed)
Proper citation: RRID:Addgene_128564 Copy
Genetic Insert: 24xMoonTag
Vector Backbone Description: Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31178119
Comments: The plasmid has 24 repeats of PP7 hairpins in 3'UTR
Proper citation: RRID:Addgene_128604 Copy
Genetic Insert: Nanobody_gp41
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31178119
Proper citation: RRID:Addgene_128603 Copy
Species: Synthetic
Genetic Insert: ECFP
Vector Backbone Description: Vector Backbone:FUW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253799
Proper citation: RRID:Addgene_128569 Copy
Genetic Insert: Nanobody_gp41
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31178119
Proper citation: RRID:Addgene_128602 Copy
Species: Homo sapiens
Genetic Insert: SHIP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4400; Vector Backbone:pTrc-HIS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9824312
Proper citation: RRID:Addgene_128568 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf172
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12860 Copy
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