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Species: Arabidopsis thaliana
Genetic Insert: CIBN-TetR-tagRFP-T
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3932; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: EGFP was cut out from the backbone (NheI/BamHI cuts); relevance of mutations unknown
Proper citation: RRID:Addgene_103809 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN-tagBFP-TetR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3932; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: EGFP was cut out from the backbone (NheI/BamHI cuts); relevance of mutations unknown
Proper citation: RRID:Addgene_103797 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN-EYFP-TetR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3932; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: EGFP was cut out from the backbone (NheI/BamHI cuts); relevance of mutations unknown
Proper citation: RRID:Addgene_103798 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-mCherry-NLS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3945; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: Nuclear localization signal (NLS) inserted by PCR
Proper citation: RRID:Addgene_103819 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN-TetR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3932; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: EGFP was cut out from the backbone (AgeI/XbaI cuts); relevance of mutations unknown
Proper citation: RRID:Addgene_103813 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN-LacI-tagRFP-T
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3932; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: EGFP was cut out from the backbone (NheI/BamHI cuts); relevance of mutations unknown
Proper citation: RRID:Addgene_103810 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-EYFP-VP16
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3935; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: aa412-482 of UL48 (transactivation domain); mCherry was cut out from the backbone (XhoI/XbaI cuts)
Proper citation: RRID:Addgene_103822 Copy
Species: Arabidopsis thaliana
Genetic Insert: cycloartenol synthase 1
Vector Backbone Description: Backbone Marker:Sergey V. Shestakov and Anne Karradt and Wolfgang Lockau; Vector Backbone:pVZ-spec; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:29281679
Comments: second gene: squalene epoxidase (SQE1)
third gene: coaR repressor protein from Synechoccystis sp. PCC6803
growth temperature (37C) referes to E. coli ; in Synechoccystis sp. PCC6803: 30 C
Proper citation: RRID:Addgene_103856 Copy
Species: Arabidopsis thaliana
Genetic Insert: marneral synthase 1
Vector Backbone Description: Backbone Marker:Sergey V. Shestakov and Anne Karradt and Wolfgang Lockau; Vector Backbone:pVZ-spec; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:29281679
Comments: second gene: squalene epoxidase (SQE1)
third gene: coaR repressor protein from Synechoccystis sp. PCC6803
growth temperature (37C) referes to E. coli ; in Synechoccystis sp. PCC6803: 30 C
Proper citation: RRID:Addgene_103858 Copy
Species: Arabidopsis thaliana
Genetic Insert: squalene epoxidase 1
Vector Backbone Description: Backbone Marker:Sergey V. Shestakov, Anne Karradt, and Wolfgang Lockau; Vector Backbone:pVZ-spec; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:29281679
Comments: also expresses coaR repressor protein from Synechoccystis sp. PCC6803
growth temperature (37C) referes to E. coli ; in Synechoccystis sp. PCC6803: 30 C
Proper citation: RRID:Addgene_103860 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2(delta2-6)-mCherry
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28916751
Proper citation: RRID:Addgene_104182 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2low-tdTomato
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28916751
Proper citation: RRID:Addgene_104067 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2high-mCherry
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28916751
Proper citation: RRID:Addgene_104063 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B (1-621 aa)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PhyB621-mCherry-HRasCT in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB621-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100282 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome interacting factor 3
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PIF3-mEGFP in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
Proper citation: RRID:Addgene_100283 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Size:4885; Vector Backbone:pL1A0*B0*_Leu; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29789561
Proper citation: RRID:Addgene_100538 Copy
Species: Arabidopsis thaliana
Genetic Insert: OEP7(1–50)
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28634853
Proper citation: RRID:Addgene_100601 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B (1-908 aa, Y276H mutation)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PhyB-Y276H-mCherry-HRasCT in mammalian cells.
PhyB-Y276H has two additional missense mutations (P840S, P884L); the construct will function as described in the publication.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100540 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTryoshka1;3-LS-F138I
Vector Backbone Description: Backbone Size:6939; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100922 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTryoshka1;3-GS-L255I
Vector Backbone Description: Backbone Size:6939; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100966 Copy
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