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Species: Other
Genetic Insert: Venus
Vector Backbone Description: Backbone Marker:Muller Lab, Addgene plasmid #83798 ; Vector Backbone:pDH22; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Venus mutations are described in "A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications" by Nagai, Ibata, Park, Kubota, Mikoshiba and Miyawaki in Nat Biotechnol 2002;20:87-90. In addition we have included Q69M, a mutation briefly mentioned in the Nagai et al. paper as potentially beneficial, although not necessary for the enhanced maturation.
Proper citation: RRID:Addgene_83800 Copy
Species: Other
Genetic Insert: CFP
Vector Backbone Description: Backbone Marker:Wach, et al. 1997, PMID 9290211; Backbone Size:4882; Vector Backbone:pFA6a-GFP(S65T)-HIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83789 Copy
Species: Other
Genetic Insert: Cerulean
Vector Backbone Description: Backbone Size:4875; Vector Backbone:pBS4 (Addgene plasmid 83793); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83795 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pBS4 (Addgene plasmid 83793); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83797 Copy
Species: Other
Genetic Insert: CFP
Vector Backbone Description: Vector Backbone:pyGFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83799 Copy
Species: Other
Genetic Insert: DsRed
Vector Backbone Description: Vector Backbone:pFA6aGFP-KanMX6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83794 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1/V5-His TOPO TA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17005694
Proper citation: RRID:Addgene_83937 Copy
Species: Other
Genetic Insert: ERT2-spCas9-ERT2
Vector Backbone Description: Backbone Marker:Sytem Biosciences; Vector Backbone:Piggybac Transposon; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that mutation R1058W in Cas9 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
The plasmid expresses a ERT2-SpCas9-ERT2 tamoxifen inducible Cas9 driven by the EF-1a promoter. T2A linked puromucin enables stable selection of the piggybac transposon. CRISPR gRNAS can be cloned into the vector at the BbsI cloning sites.
Proper citation: RRID:Addgene_83961 Copy
Species: Other
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Cre/Lox, Zebrafish transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21497092
Comments: Designed in a pBluescript series vector containing I-SceI meganuclease sites. The I-SceI meganuclease method is a common method for establishing transgenic zebrafish lines.
Please note that the Addgene verified sequence differs from the depositor's reference sequence (see supplemental materials above). The following differences do not alter plasmid functions described in the publication:
- 1kb deletion at the 5' end of the elastase promoter
- 2 other single nucleotide mismatches in elastase promoter
- L88K amino acid residue substitution in the mCherry fluor
- f1 origin is in the opposite orientation
- one mismatch in the ColE1 origin.
Proper citation: RRID:Addgene_84032 Copy
Species: Other
Genetic Insert: hSpCas9
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27472953
Comments: Please note for gRNA cloning: gRNA sequence should be constructed separately in a different u6 containing vector such as pX330, and subcloned into BamHI/NotI sites of pCRISPR-S12.
Proper citation: RRID:Addgene_84031 Copy
Species: Other
Genetic Insert: Chloramphenicol acetyltransferase
Vector Backbone Description: Backbone Marker:Ambion; Vector Backbone:pAMB; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26781350
Proper citation: RRID:Addgene_61002 Copy
Species: Other
Genetic Insert: LovTAP N538E
Vector Backbone Description: Vector Backbone:pCAL-N; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20562867
Proper citation: RRID:Addgene_61431 Copy
Species: Other
Genetic Insert: hSaCas9
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25830891
Comments: Note: This plasmid does not have an sgRNA expression cassette. Please refer to the associated publication, as well as the AAV portion of http://www.addgene.org/crispr/zhang/ , for more information on expressing sgRNAs.
Proper citation: RRID:Addgene_61594 Copy
Species: Other
Genetic Insert: N-mCherry-ME(pS)VD
Vector Backbone Description: Backbone Size:3937; Vector Backbone:pNAS1B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25272187
Proper citation: RRID:Addgene_61966 Copy
Species: Other
Genetic Insert: mRFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62247 Copy
Species: Other
Genetic Insert: mRFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62248 Copy
Species: Other
Genetic Insert: YFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62245 Copy
Species: Other
Genetic Insert: mRFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62250 Copy
Species: Other
Genetic Insert: mRFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62251 Copy
Species: Other
Genetic Insert: mCherry-BirA
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:yeast c-terminal tagging vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62388 Copy
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