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Species: Other
Genetic Insert: LF82_356
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100496 Copy
Species: Other
Genetic Insert: LF82_052
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100501 Copy
Species: Other
Genetic Insert: Lifeact-Citrine
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27703856
Proper citation: RRID:Addgene_100590 Copy
Species: Other
Genetic Insert: CMV promoter
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: Strong constitutive promoter : Human�cytomegalovirus�(CMV) immediate early enhancer and�promoter
IMPORT NOTES: Special character found in field(Comments)
Proper citation: RRID:Addgene_100690 Copy
Species: Other
Genetic Insert: PuroR
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: Puromycin resistance gene ecnoding a puromycin N-acetyl-transferase.
Proper citation: RRID:Addgene_100691 Copy
Species: Other
Genetic Insert: PuroR
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: Puromycin resistance gene ecnoding a puromycin N-acetyl-transferase.
Proper citation: RRID:Addgene_100692 Copy
Species: Other
Genetic Insert: P2A
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: p2A-Porcine teschovirus-1: self-cleaving� 2A peptide. Nat.Biotechnol.2004. 22, 589�594
IMPORT NOTES: Special character found in field(Comments)
Proper citation: RRID:Addgene_100696 Copy
Species: Other
Genetic Insert: Lentiviral chimeric 5' LTR
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: Chimeric 5'LTR for lentiviral vectors :It contains an RSV promoter, a truncated 5'LTR and other lentiviral elements such as Phi element, cPPT/CTS and RRE. Note: it contains an internal BsaI site that has not been removed to not alter the original sequence
Proper citation: RRID:Addgene_100704 Copy
Species: Other
Genetic Insert: SV40-polyA
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: SV40-polyA terminator sequence.
Proper citation: RRID:Addgene_100678 Copy
Species: Other
Genetic Insert: fusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
Vector Backbone Description: Backbone Marker:Connie Cepko; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_100800 Copy
Species: Other
Genetic Insert: MCP-YFP
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28290446
Proper citation: RRID:Addgene_101160 Copy
Species: Other
Genetic Insert: YPet(short)-HP35st-mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6238; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26523364
Proper citation: RRID:Addgene_101251 Copy
Species: Other
Genetic Insert: GPI
Vector Backbone Description: Backbone Size:5151; Vector Backbone:pMCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Originally cloned by Covalys. The GPI only is 87 bp. The size of ER signal sequence + MCP+GPI is 408 bp. The MCP-tag is N-terminal to the GPI sequence. Additional resistance to Kanamycin.
For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
Proper citation: RRID:Addgene_101132 Copy
Species: Other
Genetic Insert: YPet(short)-HP35-mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6238; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26523364
Proper citation: RRID:Addgene_101250 Copy
Species: Other
Genetic Insert: NL4-3 vpu
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101330 Copy
Species: Other
Genetic Insert: GFP, tat
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101338 Copy
Species: Other
Genetic Insert: NL4-3 envFS
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101333 Copy
Species: Other
Genetic Insert: NL4-3 envFS
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101334 Copy
Species: Other
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pNL4-3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101341 Copy
Species: Other
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pNL4-4; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Comments: The last methionine in Gag (M423) was left as it was outside the ApaI site used to clone in the mutations. However, C-terminus of Gag is functionally inert, and Pol slips 27 nucleotides away and is unlikely to produce a stable protein product.
Proper citation: RRID:Addgene_101342 Copy
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