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http://www.addgene.org/226786

Species: Danio rerio
Genetic Insert: ubiquitin promoter
Vector Backbone Description: Backbone Marker:Unknown; Backbone Size:6500; Vector Backbone:pTol2Dest-pGH_R4-R3; Vector Types:Cre/Lox, Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42403048
Comments: The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

Proper citation: RRID:Addgene_226786 Copy   

•   Source: Addgene

http://www.addgene.org/226785

Species: Danio rerio
Genetic Insert: ziwi promoter
Vector Backbone Description: Backbone Marker:Unknown; Backbone Size:9708; Vector Backbone:pTol2Dest-pGH_R4-R3; Vector Types:Cre/Lox, Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42403048
Comments: The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

Proper citation: RRID:Addgene_226785 Copy   

•   Source: Addgene

http://www.addgene.org/226788

Species: Danio rerio
Genetic Insert: ziwi promoter
Vector Backbone Description: Backbone Size:6946; Vector Backbone:pTol2Dest-pBH_R4-R2; Vector Types:Cre/Lox, Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42403048
Comments: The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

Proper citation: RRID:Addgene_226788 Copy   

•   Source: Addgene

http://www.addgene.org/226791

Species: Danio rerio
Genetic Insert: askopos promoter
Vector Backbone Description: Backbone Marker:Tol2kit v1.0; Backbone Size:2643; Vector Backbone:p5E-MCS; Vector Types:Gateway 5'-entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42403048
Comments: The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

Proper citation: RRID:Addgene_226791 Copy   

•   Source: Addgene

http://www.addgene.org/254516

Species: Danio rerio
Genetic Insert: hmx2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_254516 Copy   

•   Source: Addgene

http://www.addgene.org/254511

Species: Danio rerio
Genetic Insert: yap1S54A
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_254511 Copy   

•   Source: Addgene

http://www.addgene.org/254507

Species: Danio rerio
Genetic Insert: vgll4l
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_254507 Copy   

•   Source: Addgene

http://www.addgene.org/253619

Species: Danio rerio
Genetic Insert: Ace2N-mNeon
Vector Backbone Description: Vector Backbone:pDestTol pA2; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42109300
Comments: Please visit https://doi.org/10.1101/2024.12.12.628143 for bioRxiv preprint.

Proper citation: RRID:Addgene_253619 Copy   

•   Source: Addgene

http://www.addgene.org/226796

Species: Danio rerio
Genetic Insert: nanos2
Vector Backbone Description: Backbone Marker:Nathan Lawson; Backbone Size:2810; Vector Backbone:p5E-MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Cloned by Maya Pahima. The putative zebrafish nos2 promoter was cloned from zebrafish ovary gDNA from a region directly upstream of the nos2 coding sequence with the following primers: 3’-ACTGGCCTAAAGGGTGTCCT-5’ and 3’-CGTCCCTTGCCTTTAGTCTG-5’. The cloned amplicon was inserted into the pCR8 cloning vector using the TOPO™ TA Cloning Kit (Thermo Fisher, K250020) for stabilization and sequencing. Putative nos2 promoter region was excised from the TOPO vector using EcoRI and SmaI, treated with T4 polymerase, then inserted into SmaI-linearized p5E-MCS (Addgene, #26029).

Proper citation: RRID:Addgene_226796 Copy   

•   Source: Addgene

http://www.addgene.org/226799

Species: Danio rerio
Genetic Insert: nanos2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2640; Vector Backbone:pDONR-P2R-P3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Cloned by Maya Pahima. The putative zebrafish nos2 3'utr was cloned from zebrafish ovary gDNA using primers (3’-ATCAACCAATGCTGAATCTTTTTATAAAAAATTGAA-5' and 3’-AAATAAAATATATCAGGAGGAAACAAATATATCAGG-5') and inserted into the pCR4 cloning vector using the TOPO™ TA Cloning Kit (Thermo Fisher, K457502) for stabilization and sequencing (pCR4-nos2,3'utr). To insert the nos2,3’utr sequence into a Gateway Cloning vector, the region was amplified with attB primers (3'-GGGGACAGCTTTCTTGTACAAAGTGGTATATCAACCAATGCTGAATCTTTTTATAAAAAATTGAA-5' and 3'-GGGGACAACTTTGTATAATAAAGTTGTAAATAAAATATATCAGGAGGAAACAAATATATCAGG-5') and a BP reaction was performed with the Gateway BP Clonase II Enzyme mix (Invitrogen, 11789020) to recombine the amplicon into pDONR-P2R-P3 (Invitrogen, 12537-023) and produce p3E-nos2,3’utr.

Proper citation: RRID:Addgene_226799 Copy   

•   Source: Addgene

http://www.addgene.org/33380

Species: Danio rerio
Genetic Insert: Zinc finger array targeting HACE1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene HACE1. Please note that this plasmid does NOT contain the HACE1 sequence. Users must order the complementary plasmid HACE1_L (OZ565) [Addgene plasmid 33379] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GCTGGAGAA-3' in a bacterial two hybrid assay, and resulted in 7.96 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4 (10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)

Proper citation: RRID:Addgene_33380 Copy   

•   Source: Addgene

http://www.addgene.org/33383

Species: Danio rerio
Genetic Insert: Zinc finger array targeting dio2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dio2. Please note that this plasmid does NOT contain the dio2 sequence. Users must order the complementary plasmid dio2_R (OZ570) [Addgene plasmid 33384] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GGAGCAGCT-3' in a bacterial two hybrid assay, and resulted in 7.90 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)

Proper citation: RRID:Addgene_33383 Copy   

•   Source: Addgene

http://www.addgene.org/33384

Species: Danio rerio
Genetic Insert: Zinc finger array targeting dio2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dio2. Please note that this plasmid does NOT contain the dio2 sequence. Users must order the complementary plasmid dio2_L (OZ569) [Addgene plasmid 33383] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-TAAGGTGGT-3' in a bacterial two hybrid assay, and resulted in 8.35 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)

Proper citation: RRID:Addgene_33384 Copy   

•   Source: Addgene

http://www.addgene.org/33381

Species: Danio rerio
Genetic Insert: Zinc finger array targeting atp2c1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene atp2c1. Please note that this plasmid does NOT contain the atp2c1 sequence. Users must order the complementary plasmid atp2c1_R (OZ568) [Addgene plasmid 33382] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GTGGAGGTA-3' in a bacterial two hybrid assay, and resulted in 4.15 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)

Proper citation: RRID:Addgene_33381 Copy   

•   Source: Addgene

http://www.addgene.org/33382

Species: Danio rerio
Genetic Insert: Zinc finger array targeting atp2c1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene atp2c1. Please note that this plasmid does NOT contain the atp2c1 sequence. Users must order the complementary plasmid atp2c1_L (OZ567) [Addgene plasmid 33381] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GCTGATGGA-3' in a bacterial two hybrid assay, and resulted in 14.13 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)

Proper citation: RRID:Addgene_33382 Copy   

•   Source: Addgene

http://www.addgene.org/33377

Species: Danio rerio
Genetic Insert: Zinc finger array targeting esrrga
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene esrrga. Please note that this plasmid does NOT contain the esrrga sequence. Users must order the complementary plasmid esrrga_R (OZ564) [Addgene plasmid 33378] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GCGGTAGCT-3' in a bacterial two hybrid assay, and resulted in 4.39 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)

Proper citation: RRID:Addgene_33377 Copy   

•   Source: Addgene

http://www.addgene.org/33378

Species: Danio rerio
Genetic Insert: Zinc finger array targeting esrrga
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene esrrga. Please note that this plasmid does NOT contain the esrrga sequence. Users must order the complementary plasmid esrrga_L (OZ563) [Addgene plasmid 33377] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-TCTGAGGAG-3' in a bacterial two hybrid assay, and resulted in 5.42 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)

Proper citation: RRID:Addgene_33378 Copy   

•   Source: Addgene

http://www.addgene.org/33379

Species: Danio rerio
Genetic Insert: Zinc finger array targeting HACE1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene HACE1. Please note that this plasmid does NOT contain the HACE1 sequence. Users must order the complementary plasmid HACE1_R (OZ566) [Addgene plasmid 33380] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GGAGATGAG-3' in a bacterial two hybrid assay, and resulted in 3.04 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)

Proper citation: RRID:Addgene_33379 Copy   

•   Source: Addgene

http://www.addgene.org/35038

Species: Danio rerio
Genetic Insert: Lyn C466A-GFP
Vector Backbone Description: Vector Backbone:tol2-mpx; Vector Types:Mammalian Expression, Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22101434

Proper citation: RRID:Addgene_35038 Copy   

•   Source: Addgene

http://www.addgene.org/35037

Species: Danio rerio
Genetic Insert: Lyn-GFP
Vector Backbone Description: Vector Backbone:tol2-mpx; Vector Types:Mammalian Expression, Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22101434

Proper citation: RRID:Addgene_35037 Copy   

•   Source: Addgene