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Species: Synthetic
Genetic Insert: cAMPFIRE-M (cAMP sensor)
Vector Backbone Description: Backbone Size:5922; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182284 Copy
Species: Synthetic
Genetic Insert: cAMPFIRE-L (cAMP sensor)
Vector Backbone Description: Backbone Size:5922; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182283 Copy
Species: Synthetic
Genetic Insert: mVenusNB-MrkHR113A
Vector Backbone Description: Backbone Marker:J. H. Davis, A. J. Rubin, R. T. Sauer, Design, construction and characterization of a set of insulated bacterial promoters.; Backbone Size:3200; Vector Backbone:pEB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35131853
Comments: We obtained the codon optimized MrkH sequence from Maria Schumacher, as described in her publication PMID: 27551088
Proper citation: RRID:Addgene_182292 Copy
Species: Synthetic
Genetic Insert: ArcLight-ST
Vector Backbone Description: Backbone Size:4831; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34893595
Proper citation: RRID:Addgene_182318 Copy
Species: Synthetic
Genetic Insert: cAMPFIRE-L (cAMP sensor)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182279 Copy
Species: Synthetic
Genetic Insert: Kv-ArcLight-ST
Vector Backbone Description: Backbone Size:4794; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34893595
Proper citation: RRID:Addgene_182319 Copy
Species: Synthetic
Genetic Insert: GFP 1-9 OPT
Vector Backbone Description: Backbone Marker:pEGFP (Clontech); Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24092409
Proper citation: RRID:Addgene_182244 Copy
Species: Synthetic
Genetic Insert: T2A-tdTomato-FNF
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, FLP / FRT; Bacterial Resistance:Ampicillin
Comments: Release the insert with PmeI and PacI digest.
Proper citation: RRID:Addgene_182340 Copy
Species: Synthetic
Genetic Insert: T2A-TagRFP-T-linker-dre-PBD*-FNF
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Comments: Release the insert with PmeI and PacI digest.
Proper citation: RRID:Addgene_182342 Copy
Species: Synthetic
Genetic Insert: BSD
Vector Backbone Description: Backbone Marker:Alfred L. Fisher; Vector Backbone:pMOD4-galK-G; Vector Types:Bacterial Expression, Mouse Targeting, FLP / FRT; Bacterial Resistance:Ampicillin
Comments: PCR amplify around the 5' T2A and 3' FRT site, making sure to add a PacI site at the 3' of the FRT site.
As suggested in the plasmid name, due to a PCR error, the rpsL open reading frame has a 1-bp deletion that results in a frame shift and as a consequence premature stop codons. Despite this, the downstream BSD open reading frame is translated from the bicistronic RNA in E. coli without problems. These mean that the mutated rpsL cannot be used for streptomycin counterselection, but the unmutated BSD can and was successfully used several times to confer blasticidin resistance for selection after recombineering.
Proper citation: RRID:Addgene_182338 Copy
Species: Synthetic
Genetic Insert: T2A-td-sfGFP-FNF
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, FLP / FRT; Bacterial Resistance:Ampicillin
Comments: Release the insert with PmeI and PacI digest.
Proper citation: RRID:Addgene_182339 Copy
Species: Synthetic
Genetic Insert: 4x miR-124 target sites
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Four copies of miR-124 target sites (Åkerblom et al., 2012, https://doi.org/10.1523/JNEUROSCI.0558-12.2012) were cloned into “StLa” reporter plasmid (pCAG-loxP-pEM7-Neo*-bGHpA-3xSV40pA-loxP-tauLacZ-bGHpA from Nam and Benezra, 2009, https://doi.org/10.1016/j.stem.2009.08.017) without the tauLacZ insert and the Rosa26 homology arms. The 4x miR-124 target sequence can be released with BamHI and NotI.
More information on the “StLa” reporter at
(1) http://www.informatics.jax.org/allele/MGI:4366911
(2) https://figshare.com/ndownloader/files/38884434 (clicking this link will download a file)
Proper citation: RRID:Addgene_182363 Copy
Species: Synthetic
Genetic Insert: Myr-DeadCasp8-ERT2 and Myr-Casp8-ERT2
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox, Synthetic Biology, dre / rox , FLP / FRT; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182360 Copy
Species: Synthetic
Genetic Insert: eGFP-T2A-mCherry
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_118422 Copy
Species: Synthetic
Genetic Insert: Citrine-T2A-mTq2
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_118424 Copy
Species: Synthetic
Genetic Insert: mko2-T2A-mTq2
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_118427 Copy
Species: Synthetic
Genetic Insert: ExRai-AKAR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5412; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30250062
Proper citation: RRID:Addgene_118405 Copy
Species: Synthetic
Genetic Insert: ExRai-AKAR(T/A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5412; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30250062
Proper citation: RRID:Addgene_118406 Copy
Species: Synthetic
Genetic Insert: ytdTomato
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_118456 Copy
Species: Synthetic
Genetic Insert: ytdtomato-T2A-mTurquoise2
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_118451 Copy
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