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Species: Scherffelia dubia
Genetic Insert: sdChR(C138S)-TS-GCaMP6f-ER
Vector Backbone Description: Backbone Size:9240; Vector Backbone:FCK(1.3)GW, from Addgene 22217; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296307
Comments: sdChR was first published in: Klapoetke, N. C., Y. Murata, S.S. Kim, S.R. Pulver, A. Birdsey-Benson, Y.K. Cho, T.K. Morimoto, A.S. Chuong, E.J. Carpenter and Z. Tian. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
GCaMP6f was published in: Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen et al (Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.)
Proper citation: RRID:Addgene_58522 Copy
Species: Other
Genetic Insert: neo
Vector Backbone Description: Vector Backbone:pCEP4; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25002522
Proper citation: RRID:Addgene_58525 Copy
Species: Scherffelia dubia
Genetic Insert: sdChR(C138S, E154A)-TS-GCaMP6f-ER
Vector Backbone Description: Backbone Size:9240; Vector Backbone:FCK(1.3)GW, from Addgene 22217; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296307
Comments: sdChR was first published in: Klapoetke, N. C., Y. Murata, S.S. Kim, S.R. Pulver, A. Birdsey-Benson, Y.K. Cho, T.K. Morimoto, A.S. Chuong, E.J. Carpenter and Z. Tian. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
sdChR(E154A) was first published in: Hochbaum, Zhao, Farhi, Klapoetke, et al. 2014. "All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins." Nature Methods (in press).
GCaMP6f was published in: Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen et al (Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.)
Proper citation: RRID:Addgene_58524 Copy
Species: Other
Genetic Insert: neo
Vector Backbone Description: Vector Backbone:pCEP4; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25002522
Proper citation: RRID:Addgene_58526 Copy
Species: Mus musculus
Genetic Insert: JunD
Vector Backbone Description: Backbone Marker:Weinberg Lab (Addgene plasmid 1767) ; Backbone Size:5300; Vector Backbone:pBabe neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24658685
Comments: pBabe JunD_HA neo was constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe neo. The PCR product was gel purified, digested with BamHI and EcoRI, and then ligated into pBabe neo, which had been digested with BamHI and EcoRI and then dephosphorylated.
Proper citation: RRID:Addgene_58486 Copy
Species: T. gondii
Genetic Insert: DHFR cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4555; Vector Backbone:pDEST R4-R3 Vector II; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24825012
Proper citation: RRID:Addgene_58528 Copy
Species: Mus musculus
Genetic Insert: IFITM3
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:3800; Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22511783
Proper citation: RRID:Addgene_58413 Copy
Species: Mus musculus
Genetic Insert: IFITM1
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:3800; Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23804635
Proper citation: RRID:Addgene_58415 Copy
Species: Homo sapiens
Genetic Insert: KRT5
Vector Backbone Description: Backbone Size:6265; Vector Backbone:pBabe N-terminal RFP hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24658685
Comments: pBabe RFP1_KRT5 hygro was constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe N-terminal RFP hygro. The PCR product was gel purified, digested with BamHI and EcoRI, and then ligated into pBabe N-terminal RFP hygro, which had been digested with BamHI and EcoRI and then dephosphorylated.
Proper citation: RRID:Addgene_58493 Copy
Species: Homo sapiens
Genetic Insert: IRE1a
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23487760
Proper citation: RRID:Addgene_58492 Copy
Species: Mus musculus
Genetic Insert: JunD
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pBabe C-terminal Venus puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24658685
Comments: pBabe JunD_Venus puro was constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe C-terminal Venus puro. The PCR product was gel purified, digested with BamHI and EcoRI, and then ligated into pBabe c-terminal Venus puro, which had been digested with BamHI and EcoRI and then dephosphorylated.
Proper citation: RRID:Addgene_58496 Copy
Species: Mus musculus
Genetic Insert: IFITM1
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:3800; Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23804635
Proper citation: RRID:Addgene_58418 Copy
Species: Homo sapiens
Genetic Insert: IRE1a
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23487760
Proper citation: RRID:Addgene_58422 Copy
Species: Mus musculus
Genetic Insert: IFITM1
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:3800; Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23804635
Proper citation: RRID:Addgene_58421 Copy
Species: Mus musculus
Genetic Insert: eGFP-omp25
Vector Backbone Description: Vector Backbone:pclBW; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24703695
Proper citation: RRID:Addgene_58424 Copy
Species: Mus musculus
Genetic Insert: PERK
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23487760
Proper citation: RRID:Addgene_58423 Copy
Genetic Insert: mito-CFP
Vector Backbone Description: Backbone Marker:gifted by C. Lois and D. Baltimore; Vector Backbone:pclbw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24703695
Proper citation: RRID:Addgene_58426 Copy
Genetic Insert: mito-tagRFP
Vector Backbone Description: Backbone Marker:gifted by C. Lois and D. Baltimore; Vector Backbone:pclbw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24703695
Proper citation: RRID:Addgene_58425 Copy
Species: Synthetic
Genetic Insert: CitrineA206K
Vector Backbone Description: Backbone Marker:Robert Gnuegge; Backbone Size:4663; Vector Backbone:pRS-derived; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25034689
Comments: For integration in yeast, linearize plasmid with PacI.
Proper citation: RRID:Addgene_58433 Copy
Species: Synthetic
Genetic Insert: CitrineA206K
Vector Backbone Description: Backbone Marker:Robert Gnuegge; Backbone Size:4663; Vector Backbone:pRS-derived; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25034689
Comments: For integration in yeast, linearize plasmid with PacI.
Proper citation: RRID:Addgene_58432 Copy
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