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  • Organism:saccharomyces cerevisiae (facet)

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pYSD288
 
Resource Report
Resource Website 		RRID:Addgene_253023 AGA1 Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:41 0
pYSD291
 
Resource Report
Resource Website 		RRID:Addgene_253024 AGA1 Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:41 0
pYSD311
 
Resource Report
Resource Website 		RRID:Addgene_253033 mRuby2-Aga2 Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:41 0
Srs2-C-terminus_pET28a
 
Resource Report
Resource Website 		RRID:Addgene_247774 Srs2-C-terminus Saccharomyces cerevisiae Kanamycin Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin The C-terminus of the gene has Lys-to-Arg mutations 2026-08-15 01:35:44 0
pET29b(+)_SUMO(1-98)_sTEV_His6
 
Resource Report
Resource Website 		RRID:Addgene_254139 SUMO family protein SMT3 Saccharomyces cerevisiae Kanamycin PMID:41927759 This plasmid contains a SUMO protein construct to serve as a control protein for binding experiments using the AH-SUMO platform, to verify non-interaction between the SUMO domain and liposomes of choice. It contains the SUMO1-98 region of the SMT3_yeast gene, followed by a Cysteine, a TEV site and a hexa-histidine site. On the N-terminal of the SUMO domain, it contains a region encoding for MGSGS, which is there to mimic the structure of a AH-SUMO platform that contains an N-terminal peptide of interest , serving as a better control. Please visit https://doi.org/10.1101/2025.05.12.653516 for the bioRxiv preprint. Backbone Size:5228; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Contains MGSGS sequence on N-terminal of SMT3_yeast SUMO(1-98) gene. Gene is followed by a Cysteine, a TEV site and a hexahistidine tag. 2026-08-15 01:35:46 0
pYSD728
 
Resource Report
Resource Website 		RRID:Addgene_253050 Leaf compost cutinase Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:45 0
pYSD890
 
Resource Report
Resource Website 		RRID:Addgene_253016 MET15 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:45 0
pYSD297
 
Resource Report
Resource Website 		RRID:Addgene_253029 AGA1 Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:48 0
YIplac211-Sys1-msGFP2-FRBx2
 
Resource Report
Resource Website 		RRID:Addgene_247749 Sys1-msGFP2-FRBx2 Saccharomyces cerevisiae Ampicillin Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:34:39 0
YIplac211-Kex2-FRBx2-msGFPx3
 
Resource Report
Resource Website 		RRID:Addgene_247739 Kex2-FRBx2-msGFPx3 Saccharomyces cerevisiae Ampicillin Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:34:39 0
YIplac211-VPS54-msGFP2x3
 
Resource Report
Resource Website 		RRID:Addgene_255068 Vps54-msGFP2x3 Saccharomyces cerevisiae Ampicillin Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:03 0
Sc FLAG-Pol1(STS)-pRS402/GAL-L
 
Resource Report
Resource Website 		RRID:Addgene_257522 Pol1 Saccharomyces cerevisiae Ampicillin Vector Backbone:pRS402/GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin D996S; D998S (=STS) 2026-08-15 01:36:03 0
Sc RFC[2 + 3(GAT/SAC) + 4]-pET
 
Resource Report
Resource Website 		RRID:Addgene_258197 RFC2 Saccharomyces cerevisiae Ampicillin Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:07 0
Sc RFC[2(SAC) + 3 + 4]-pET
 
Resource Report
Resource Website 		RRID:Addgene_258199 RFC2 Saccharomyces cerevisiae Ampicillin Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin RFC2 (R183A) (SAC) 2026-08-15 01:36:08 0
Sc RFC[2 + 3 + 4(GAT)]-pET
 
Resource Report
Resource Website 		RRID:Addgene_258190 RFC2 Saccharomyces cerevisiae Ampicillin Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:08 0
Sc RFC[2 + 3(SAC) + 4(SAC)]-pET
 
Resource Report
Resource Website 		RRID:Addgene_258193 RFC2 Saccharomyces cerevisiae Ampicillin Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:08 0
Sc RFC[2 + 3 + 4(GAT/SAC)]-pET
 
Resource Report
Resource Website 		RRID:Addgene_258191 RFC2 Saccharomyces cerevisiae Ampicillin Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:07 0
Sc RFC[2 + 3(SAC) + 4]-pET
 
Resource Report
Resource Website 		RRID:Addgene_258192 RFC2 Saccharomyces cerevisiae Ampicillin Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:07 0
Sc Top2-FLAG-pRS402/GAL-L
 
Resource Report
Resource Website 		RRID:Addgene_257905 Sc Top2 Saccharomyces cerevisiae Ampicillin Vector Backbone:pRS402/GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:08 0
Sc FLAG-Top1-pRS404/GAL-L
 
Resource Report
Resource Website 		RRID:Addgene_257904 Sc Top1 Saccharomyces cerevisiae Ampicillin Vector Backbone:pRS404/GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:06 0

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