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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCMV-myc-Atg7 Resource Report Resource Website 10+ mentions |
RRID:Addgene_24921 | Atg7 | Homo sapiens | Ampicillin | PMID:18296641 | Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:58 | 12 | ||
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F-CDK8L Resource Report Resource Website 1+ mentions |
RRID:Addgene_24762 | F-CDK8L | Homo sapiens | Ampicillin | PMID:15680972 | Backbone Marker:Addgene plasmid 15439; Backbone Size:5600; Vector Backbone:pcDNA3.1 N-FLAG/Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:57 | 3 | ||
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human pirh2-(138-261) Resource Report Resource Website |
RRID:Addgene_24882 | Pirh2 | Homo sapiens | Ampicillin | PMID:19043414 | E. coli strain for expression: BL21DE3 Expression conditions: Pirh2 proteins need Zn2+ to fold properly, so 50 uM of ZnCl2 is added to the growth media and purification buffers. Induction conditions: 0.5mM-1.0mM IPTG at RT for 3.5-4 hours. For pGEX-2TKm vector, the kinase site RRASV is removed and NdeI site is inserted at 5' of BamHI. | Backbone Size:0; Vector Backbone:pGEX-2TKm; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Contains aa 138-261. | Addgene | 2026-10-31 02:07:58 | 0 |
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pMSCVFLAG-ZFP423 Resource Report Resource Website 1+ mentions |
RRID:Addgene_24764 | Zinc Finger Protein 423 | Mus musculus | Ampicillin | PMID:20200519 | Backbone Marker:Clontech; Backbone Size:6295; Vector Backbone:pMSCV puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:57 | 5 | ||
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3XFlag pCMV5-TOPO TAZ (106∂) Resource Report Resource Website 1+ mentions |
RRID:Addgene_24814 | TAZ | Homo sapiens | Ampicillin | PMID:18568018 | Backbone Size:5000; Vector Backbone:pCMV5-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Deletion of aa 1-106 | Addgene | 2026-10-31 02:07:57 | 1 | |
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pGFP-Rab8A Resource Report Resource Website 10+ mentions |
RRID:Addgene_24898 | hRab8A | Homo sapiens | Ampicillin | PMID:17574030 | Backbone Size:6100; Vector Backbone:pDEST53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:58 | 13 | ||
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pTACT1 Resource Report Resource Website |
RRID:Addgene_24779 | TetR | Bacterial | Kanamycin | PMID:15933004 | There are a few mismatches between depositor's sequence and the quality control sequence Addgene has obtained. The region of mismatch and gaps is derived from the parental vector and not the important part of the vector. Infect Immun. 2001 Dec;69(12):7851-7. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Bateman BT, Donegan NP, Jarry TM, Palma M, Cheung AL. Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA. Abstract An inducible promoter system provides a powerful tool for studying the genetic basis for virulence. A variety of inducible systems have been used in other organisms, including pXyl-xylR-inducible promoter, the pSpac-lacI system, and the arabinose-inducible P(BAD) promoter, but each of these systems has limitations in its application to Staphylococcus aureus. In this study, we demonstrated the efficacy of a tetracycline-inducible promoter system in inducing gene expression in S. aureus in vitro and inside epithelial cells as well as in an animal model of infection. Using the xyl/tetO promoter::gfp(uvr) fusion carried on a shuttle plasmid, we demonstrated that dose-dependent tetracycline induction, as measured by bacterial fluorescence, occurred in each of the above environments while basal activation under noninduced conditions remained low. To ascertain how the system can be used to elucidate the genetic basis of a pathogenic phenotype, we cloned the sigB gene downstream of the inducible promoter. Induction of SigB expression led to dose-dependent attachment of the tested strain to polystyrene microtiter wells. Additionally, bacterial microcolony formation, an event preceding mature biofilm formation, also increased with tetracycline induction of SigB. PMID: 11705967 [PubMed - indexed for MEDLINE]PMCID: PMC98881Free PMC Articl | Backbone Size:4120; Vector Backbone:pMV206; Vector Types:Mycobacterial shuttle vector; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:07:57 | 0 | |
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pGFP-Rab8A[T22N] Resource Report Resource Website 1+ mentions |
RRID:Addgene_24899 | hRab8A[T22N] | Homo sapiens | Ampicillin | PMID:17574030 | Backbone Size:6100; Vector Backbone:pDEST53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Changed Threonine 22 to Asparagine | Addgene | 2026-10-31 02:07:58 | 6 | |
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GST-p35 Resource Report Resource Website |
RRID:Addgene_24895 | p35 | Homo sapiens | Ampicillin | PMID:11813240 | Backbone Size:4969; Vector Backbone:pGEX6P-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Full-length p35 | Addgene | 2026-10-31 02:07:58 | 0 | |
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pENTR-fishRab8B[Q67L] Resource Report Resource Website |
RRID:Addgene_24908 | Rab8B | Danio rerio | Kanamycin | PMID:17574030 | This construct was generated by gene synthesis and is based on a reference sequence from 2007. Amino acids NE 178-179 are missing compared to current NCBI sequences. | Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Changed Glutamine 67 to Leucine | Addgene | 2026-10-31 02:07:58 | 0 |
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pENTR-fishRab8B Resource Report Resource Website |
RRID:Addgene_24907 | fishRab8B | Danio rerio | Kanamycin | PMID:17574030 | This construct was generated by gene synthesis and is based on a reference sequence from 2007. Amino acids NE 178-179 are missing compared to current NCBI sequences. | Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:07:58 | 0 | |
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p5189 pSUPER-shRNA-GFP Resource Report Resource Website |
RRID:Addgene_24747 | shRNA-GFP | Homo sapiens | Ampicillin | PMID:16611886 | shRNA oligo sequence is - 5′-GCAAGCTGACCCTGAAGTTC | Backbone Marker:Halo-Bio RNAi Therapeutics; Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:57 | 0 | |
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human p53-(82-360) Resource Report Resource Website |
RRID:Addgene_24868 | p53 | Homo sapiens | Ampicillin | PMID:11524676 | E.Coli strain for epression: BL21DE3-pLysS p53 proteins are expressed better and more stable at low temperature | Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Contains aa 82-360 | Addgene | 2026-10-31 02:07:58 | 0 |
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pDONR221-hSOX10 Resource Report Resource Website 1+ mentions |
RRID:Addgene_24749 | (sex determining region Y)-box 10 | Homo sapiens | Kanamycin | PMID:19422606 | Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | wildtype sequence | Addgene | 2026-10-31 02:07:57 | 6 | |
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p6042 pcDNA-Xpress-His-USP5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_24744 | USP5 | Homo sapiens | Ampicillin | PMID:19553310 | Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA-4C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:57 | 1 | ||
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human p53-(1-320) Resource Report Resource Website 1+ mentions |
RRID:Addgene_24864 | p53 | Homo sapiens | Ampicillin | PMID:11524676 | E.Coli strain for epression: BL21DE3-pLysS p53 proteins are expressed better and more stable at low temperature | Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Contains aa 1-320 | Addgene | 2026-10-31 02:07:58 | 5 |
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p6109 pSG5-HPV31-E2(HA) Resource Report Resource Website |
RRID:Addgene_24740 | HPV31 E2 (HA) | HPV31 | Ampicillin | PMID:20181716 | Backbone Marker:Stratagene; Backbone Size:4141; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:57 | 0 | ||
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pGEX-human p53-(1-393) Resource Report Resource Website 1+ mentions |
RRID:Addgene_24860 | p53 | Homo sapiens | Ampicillin | PMID:11524676 | E.Coli strain for epression: BL21DE3 Induction condition: 0.3 mM IPTG at RT for 3 hours or 15 degrees Celsius, overnight. NdeI site is inserted at 5'-end of BamHI site on pGEX-2TK vector. | Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:58 | 5 | |
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p6111 pSG5-HPV31-KWK-E8^E2C(HA) Resource Report Resource Website |
RRID:Addgene_24742 | HPV31 KWK E8^E2C (HA) | HPV31 | Ampicillin | PMID:20181716 | Backbone Marker:Stratagene; Backbone Size:4141; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | residues 5 to 8 from KWK to AEA | Addgene | 2026-10-31 02:07:57 | 0 | |
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p6110 pSG5-HPV31-E8^E2C(HA) Resource Report Resource Website |
RRID:Addgene_24741 | HPV31 E8^E2C (HA) | HPV31 | Ampicillin | PMID:20181716 | Backbone Marker:Stratagene; Backbone Size:4141; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:57 | 0 |
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