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Species: Other
Genetic Insert: intB13 integrase casette
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30853419
Proper citation: RRID:Addgene_120230 Copy
Species: Other
Genetic Insert: sfGFP/mScarletP2A-mcs
Vector Backbone Description: Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that sfGFP can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A
The associated publication utilized mNeon instead of sfGFP/mClover3, but mNeon has is exceedingly difficult to share. Therefore, these two plasmids (120261 and 120262) are made available, using sfGFP and mClover3 instead of mNeon. These plasmids have been tested, and are equivalent to the mNeon variant.
Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_120261 Copy
Species: Other
Genetic Insert: N-terminal fragment of SpyCas9
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120293 Copy
Species: Other
Genetic Insert: C-terminal fragment of SpyCas9
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120294 Copy
Species: Other
Genetic Insert: C-terminal fragment of SpyCas9
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120295 Copy
Species: Other
Genetic Insert: AcrIIA4
Vector Backbone Description: Vector Backbone:scAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120297 Copy
Species: Other
Genetic Insert: mClover
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Comments: D103N mutation in mClover does not affect function.
Proper citation: RRID:Addgene_120330 Copy
Species: Other
Genetic Insert: AcrIIA4-2xmiR-122 binding sites
Vector Backbone Description: Vector Backbone:scAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120298 Copy
Species: Other
Genetic Insert: mWasabi
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Proper citation: RRID:Addgene_120332 Copy
Species: Other
Genetic Insert: mCardinal
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Proper citation: RRID:Addgene_120334 Copy
Species: Other
Genetic Insert: mNeptune2
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Proper citation: RRID:Addgene_120335 Copy
Species: Other
Genetic Insert: mNeptune2
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Proper citation: RRID:Addgene_120336 Copy
Species: Other
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Proper citation: RRID:Addgene_120327 Copy
Species: Other
Genetic Insert: mClover
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Comments: D103N mutation in mClover does not affect plasmid function.
Proper citation: RRID:Addgene_120329 Copy
Species: Other
Genetic Insert: AcrIIC3
Vector Backbone Description: Vector Backbone:scAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120301 Copy
Species: Other
Genetic Insert: AcrIIC1-2xmiR-122 binding sites
Vector Backbone Description: Vector Backbone:scAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120302 Copy
Species: Other
Genetic Insert: Lyn-R-GECO1
Vector Backbone Description: Backbone Size:3930; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27190091
Proper citation: RRID:Addgene_120410 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:9514; Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30448000
Comments: 5' cloning site: BamHI (not destroyed), 3' cloning site: BamHI (not destroyed).
Proper citation: RRID:Addgene_120426 Copy
Species: Other
Genetic Insert: Smp_031880
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6686; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120602 Copy
Species: Other
Genetic Insert: T1-3_Lip2
Vector Backbone Description: Vector Backbone:pCR4Blunt-TOPO; Vector Types:Cloning/assembly plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31148366
Proper citation: RRID:Addgene_120777 Copy
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