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Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180328 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180329 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*35:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00237 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180450 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*02:01 (CD8 mut)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Mutation prevents binding of CD8 (Purbhoo et al. (2001) J. Biol. Chem. doi.org/10.1074/jbc.M102498200). See https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00005 for allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180459 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180334 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180339 Copy
Genetic Insert: E2 Crimson
Vector Backbone Description: Backbone Marker:Jeffrey Barrick (Addgene plasmid # 110598); Vector Backbone:pBTK402; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35467987
Comments: Please note: Plasmid contains an 85bp discrepancy downstream of E2 Crimson at the BBa_B0015 Terminator compared to the depositor's provided sequence. This discrepancy is not known to affect plasmid function.
Proper citation: RRID:Addgene_180421 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180319 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180340 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*40:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00291 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180463 Copy
Vector Backbone Description: Vector Backbone:pGL198; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34837319
Proper citation: RRID:Addgene_180472 Copy
Species: Homo sapiens
Genetic Insert: ddGFP sensor of Kras
Vector Backbone Description: Vector Backbone:pCMV-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30643148
Proper citation: RRID:Addgene_180571 Copy
Species: Homo sapiens
Genetic Insert: ddRFP sensor of Kras
Vector Backbone Description: Vector Backbone:pCMV-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30643148
Proper citation: RRID:Addgene_180572 Copy
Species: Homo sapiens
Genetic Insert: ddGFP sensor of Cdc42
Vector Backbone Description: Vector Backbone:pCMV-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30643148
Proper citation: RRID:Addgene_180577 Copy
Species: Homo sapiens
Genetic Insert: ddGFP sensor of Hras
Vector Backbone Description: Vector Backbone:pCMV-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30643148
Proper citation: RRID:Addgene_180573 Copy
Species: Homo sapiens
Genetic Insert: ddRFP sensor of Rac1
Vector Backbone Description: Vector Backbone:pCMV-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30643148
Proper citation: RRID:Addgene_180576 Copy
Species: Homo sapiens
Genetic Insert: RA-Hras and Lyn-iRFP682
Vector Backbone Description: Vector Backbone:pCMV-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30643148
Proper citation: RRID:Addgene_180584 Copy
Species: Homo sapiens
Genetic Insert: GCP60
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16870622
Proper citation: RRID:Addgene_180633 Copy
Species: Homo sapiens
Genetic Insert: golgin-160
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10791974
Proper citation: RRID:Addgene_180630 Copy
Species: Homo sapiens
Genetic Insert: golgin-160/3DE
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10791974
Comments: Caspase-resistant golgin-160
Proper citation: RRID:Addgene_180631 Copy
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