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Species: Homo sapiens
Genetic Insert: shJUND
Vector Backbone Description: Backbone Marker:Weinberg Lab (Addgene plasmid 8453) ; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24658685
Comments: This construct was obtained from the TRC (#TRCN0000014974) and potently knocks down junD expression after 3 x 500 microL infection in MCF10A cells.
The specificity of this construct has been confirmed by addback with mouse junD, which is resistant to this shRNA.
Proper citation: RRID:Addgene_58698 Copy
Genetic Insert: mV-NcWC1-LOV
Vector Backbone Description: Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24986882
Proper citation: RRID:Addgene_58691 Copy
Species: Homo sapiens
Genetic Insert: D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
Vector Backbone Description: Backbone Size:6412; Vector Backbone:Plasmid 42235: pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A); Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26126518
Proper citation: RRID:Addgene_58695 Copy
Genetic Insert: Opto-mFGFR1
Vector Backbone Description: Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24986882
Proper citation: RRID:Addgene_58745 Copy
Genetic Insert: Opto-hRET
Vector Backbone Description: Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24986882
Proper citation: RRID:Addgene_58747 Copy
Genetic Insert: Opto-hEGFR
Vector Backbone Description: Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24986882
Proper citation: RRID:Addgene_58746 Copy
Species: measles virus
Genetic Insert: Measles virus antigenome
Vector Backbone Description: Vector Backbone:p(+)MV15894; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Unique AatII site noted in the Modifications to Backbone field was confirmed by restriction digest. Please see Notes from Addgene link to view results.
Proper citation: RRID:Addgene_58748 Copy
Genetic Insert: dtA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24828191
Proper citation: RRID:Addgene_58536 Copy
Species: Aquorea victoria
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Backbone Size:3979; Vector Backbone:pJ401; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Alternative name: p007-amp-GFP-T5lac Please visit https://www.biorxiv.org/content/10.1101/2021.06.20.449138v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_58535 Copy
Species: Homo sapiens
Genetic Insert: CD4
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24627473
Comments: Note: Addgene's quality control sequencing has found an R318G amino acid residue substitution in CD4. The affect on protein function is not known.
Proper citation: RRID:Addgene_58538 Copy
Species: Homo sapiens
Genetic Insert: CD4
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24627473
Proper citation: RRID:Addgene_58537 Copy
Genetic Insert: Enhanced Green Fluorescence Protein, Puromycin resistance gene
Vector Backbone Description: Backbone Size:4941; Vector Backbone:HSC1 retroviral vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23752310
Proper citation: RRID:Addgene_58542 Copy
Species: Mus musculus
Genetic Insert: ptc
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS+; Vector Types:Bacterial Expression, in situ probe; Bacterial Resistance:Ampicillin
Comments: For sense: linearize with HindIII, transcribe with T7.
For anti-sense: linearize with BamHI, transcribe with T3.
Proper citation: RRID:Addgene_58701 Copy
Genetic Insert: Enhanced Green Fluorescence Protein, Puromycin resistance gene
Vector Backbone Description: Backbone Size:4941; Vector Backbone:HSC1 retroviral vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23752310
Proper citation: RRID:Addgene_58540 Copy
Species: Mus musculus
Genetic Insert: Ptch
Vector Backbone Description: Vector Backbone:pMal; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17641202
Comments: See antibody production protocol in supplemental documents.
Proper citation: RRID:Addgene_58705 Copy
Species: Homo sapiens
Genetic Insert: shPIK3CD
Vector Backbone Description: Backbone Marker:Weinberg Lab (Addgene plasmid 8453) ; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24449900
Comments: The primers were generated by the TRC (#TRCN0000033276) Construct mildly knocks down p110d (PIK3CD) protein expression ~2 fold after 3 X 500 uL infection in MCF10A-5E cells.
The specificity of this construct has not been confirmed by addback, but a second hairpin against the same target yielded the same phenotype.
Proper citation: RRID:Addgene_58707 Copy
Species: Homo sapiens
Genetic Insert: Oxysterol-binding Protein PH domain
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9651677
Comments: PH domain of OSBP= Serine 87 - Lysine 185
Proper citation: RRID:Addgene_58840 Copy
Species: Chloromonas subdivisa
Genetic Insert: CsChR-GFP
Vector Backbone Description: Backbone Size:4368; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: Plasmid is sequenced completely by the depositing lab except for part of the 5' ITR.
Proper citation: RRID:Addgene_58841 Copy
Species: Leptosphaeria maculans
Genetic Insert: Mac-GFP
Vector Backbone Description: Backbone Size:4708; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20054397
Comments: Plasmid is completely sequenced by the depositing lab except parts of the origin and both ITRs. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_58853 Copy
Species: Leptosphaeria maculans
Genetic Insert: Mac-GFP
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:5014; Vector Backbone:AAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20054397
Comments: Part of plasmid is sequenced by the depositing lab; the promoter, gene and fluorophore were sequenced. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_58852 Copy
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