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  • Organism:danio rerio (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCRII-TOPO-mogat2 in situ probe
 
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RRID:Addgene_232139 mogat2 Danio rerio Ampicillin and Kanamycin PMID:39510175 Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:31:47 0
pCRII-TOPO-dgat1b in situ probe
 
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RRID:Addgene_232138 dgat1b Danio rerio Ampicillin and Kanamycin PMID:39510175 Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:31:47 0
pMT- eef1a1l1:sfGFP
 
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RRID:Addgene_254856 eef1a1l1 Danio rerio Ampicillin Backbone Marker:Sean Megason; Backbone Size:4314; Vector Backbone:pMTB Addgene #112225; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:36:16 0
pTol2Dest_ubiquitin:loxp-stop-loxp-mCherry_pGH
 
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RRID:Addgene_226786 ubiquitin promoter Danio rerio Ampicillin PMID:42403048 The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Backbone Marker:Unknown; Backbone Size:6500; Vector Backbone:pTol2Dest-pGH_R4-R3; Vector Types:Cre/Lox, Zebrafish expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:26 0
pTol2Dest_ziwi:loxp-stop-loxp-mCherry_pGH
 
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RRID:Addgene_226785 ziwi promoter Danio rerio Ampicillin PMID:42403048 The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Backbone Marker:Unknown; Backbone Size:9708; Vector Backbone:pTol2Dest-pGH_R4-R3; Vector Types:Cre/Lox, Zebrafish expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:23 0
pTol2Dest_ziwi:iCre_pBH
 
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RRID:Addgene_226788 ziwi promoter Danio rerio Ampicillin PMID:42403048 The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Backbone Size:6946; Vector Backbone:pTol2Dest-pBH_R4-R2; Vector Types:Cre/Lox, Zebrafish expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:23 0
p5E-askopos
 
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RRID:Addgene_226791 askopos promoter Danio rerio Kanamycin PMID:42403048 The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Backbone Marker:Tol2kit v1.0; Backbone Size:2643; Vector Backbone:p5E-MCS; Vector Types:Gateway 5'-entry vector; Bacterial Resistance:Kanamycin 2026-08-15 01:36:23 0
mNeonAce-unc45
 
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RRID:Addgene_253619 Ace2N-mNeon Danio rerio Ampicillin PMID:42109300 Please visit https://doi.org/10.1101/2024.12.12.628143 for bioRxiv preprint. Vector Backbone:pDestTol pA2; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:27 0
p5E-nos2 promoter_L4-R1
 
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RRID:Addgene_226796 nanos2 Danio rerio Kanamycin Cloned by Maya Pahima. The putative zebrafish nos2 promoter was cloned from zebrafish ovary gDNA from a region directly upstream of the nos2 coding sequence with the following primers: 3’-ACTGGCCTAAAGGGTGTCCT-5’ and 3’-CGTCCCTTGCCTTTAGTCTG-5’. The cloned amplicon was inserted into the pCR8 cloning vector using the TOPO™ TA Cloning Kit (Thermo Fisher, K250020) for stabilization and sequencing. Putative nos2 promoter region was excised from the TOPO vector using EcoRI and SmaI, treated with T4 polymerase, then inserted into SmaI-linearized p5E-MCS (Addgene, #26029). Backbone Marker:Nathan Lawson; Backbone Size:2810; Vector Backbone:p5E-MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:36:26 0
p3E-nanos2 3'utr_R2-R3
 
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RRID:Addgene_226799 nanos2 Danio rerio Kanamycin Cloned by Maya Pahima. The putative zebrafish nos2 3'utr was cloned from zebrafish ovary gDNA using primers (3’-ATCAACCAATGCTGAATCTTTTTATAAAAAATTGAA-5' and 3’-AAATAAAATATATCAGGAGGAAACAAATATATCAGG-5') and inserted into the pCR4 cloning vector using the TOPO™ TA Cloning Kit (Thermo Fisher, K457502) for stabilization and sequencing (pCR4-nos2,3'utr). To insert the nos2,3’utr sequence into a Gateway Cloning vector, the region was amplified with attB primers (3'-GGGGACAGCTTTCTTGTACAAAGTGGTATATCAACCAATGCTGAATCTTTTTATAAAAAATTGAA-5' and 3'-GGGGACAACTTTGTATAATAAAGTTGTAAATAAAATATATCAGGAGGAAACAAATATATCAGG-5') and a BP reaction was performed with the Gateway BP Clonase II Enzyme mix (Invitrogen, 11789020) to recombine the amplicon into pDONR-P2R-P3 (Invitrogen, 12537-023) and produce p3E-nos2,3’utr. Backbone Marker:Invitrogen; Backbone Size:2640; Vector Backbone:pDONR-P2R-P3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:36:26 0
PCRII-zSynA
 
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RRID:Addgene_20121 zSynA Danio rerio Ampicillin zSynA in situ probe. For antisense, cut with BamHI and transcribe with T7 Backbone Marker:Invitrogen; Backbone Size:3961; Vector Backbone:PCRII-TOPO; Vector Types:in-situ; Bacterial Resistance:Ampicillin 2026-08-15 01:11:51 0
pT2KXIGdeltaIn-MCS-birA-tagRFP
 
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1+ mentions
RRID:Addgene_58378 birA tagRFP Danio rerio Ampicillin PMID:25231762 Note: Addgene's quality control sequencing has found some discrepancies with the depositor's uploaded sequence. The depositing lab is aware of these differences, and they are not thought to affect plasmid function. Backbone Marker:Kawakami; Backbone Size:6173; Vector Backbone:pT2KXIGdeltaIn; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:17:15 1
pT2KXIGdeltaIn-MCS-birA-EGFP
 
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RRID:Addgene_58377 birA EGFP Danio rerio Ampicillin PMID:25231762 Note: Addgene's quality control sequencing has found some discrepancies with the depositor's uploaded sequence. The depositing lab is aware of these differences, and they are not thought to affect plasmid function. Backbone Marker:Kawakami; Backbone Size:6208; Vector Backbone:pT2KXIGdeltaIn; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:17:15 0
pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a
 
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1+ mentions
RRID:Addgene_58380 avi-EGFP-rpl10a Danio rerio Ampicillin PMID:25231762 Note: Addgene's quality control sequencing has found some discrepancies with the depositor's uploaded sequence. The depositing lab is aware of these differences, and they are not thought to affect plasmid function. Backbone Marker:Kawakami; Backbone Size:6173; Vector Backbone:pT2KXIGdeltaIn; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:17:15 1
p5'Entry-lck-5.5kb
 
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1+ mentions
RRID:Addgene_58890 zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb Danio rerio Kanamycin Backbone Marker:Invitrogen; Backbone Size:4777; Vector Backbone:pDONR P4-P1R; Vector Types:Gateway 5' entry clone; Bacterial Resistance:Kanamycin mutated single nucleotide (T-->C, 13th nucleotide of insert) to create novel SfiI site at 5' end of promoter 2026-08-15 01:17:19 1
Tol2-elavl3-GCaMP6s
 
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1+ mentions
RRID:Addgene_59531 elavl3 promoter Danio rerio Ampicillin PMID:25068735 Addgene NGS QC analysis identified multiple variants in the HuC promoter sequence. Vector Backbone:MiniTol2; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:17:23 7
Tol2-elavl3-H2B-GCaMP6s
 
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10+ mentions
RRID:Addgene_59530 elavl3 promoter Danio rerio Ampicillin PMID:25068736 Addgene NGS QC analysis identified multiple variants in the HuC promoter sequence. Vector Backbone:MiniTol2; Vector Types:Transposable element; Bacterial Resistance:Ampicillin 2026-08-15 01:17:23 14
pCRII HSPB15
 
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RRID:Addgene_61039 HSPB15 Danio rerio Ampicillin PMID:18161059 linearize with NotI, transcribe with SP6, probe length is 556. Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:38 0
pCRII HSPB9
 
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RRID:Addgene_61038 HSPB9 Danio rerio Ampicillin PMID:18161059 linearize with NotI, transcribe with SP6, probe length is 556. Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:38 0
pCRII HSPB12
 
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RRID:Addgene_61037 HSPB12 Danio rerio Ampicillin PMID:18161059 linearize with NotI, transcribe with SP6, probe length is 523. Note: Addgene's quality control sequencing has found a few mismatches in the plasmid insert, but they should not affect its ability to be used as a probe. Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:38 0

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