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Species: Danio rerio
Genetic Insert: mogat2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:39510175
Proper citation: RRID:Addgene_232139 Copy
Species: Danio rerio
Genetic Insert: dgat1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:39510175
Proper citation: RRID:Addgene_232138 Copy
Species: Danio rerio
Genetic Insert: eef1a1l1
Vector Backbone Description: Backbone Marker:Sean Megason; Backbone Size:4314; Vector Backbone:pMTB Addgene #112225; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_254856 Copy
Species: Danio rerio
Genetic Insert: ubiquitin promoter
Vector Backbone Description: Backbone Marker:Unknown; Backbone Size:6500; Vector Backbone:pTol2Dest-pGH_R4-R3; Vector Types:Cre/Lox, Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42403048
Comments: The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
Proper citation: RRID:Addgene_226786 Copy
Species: Danio rerio
Genetic Insert: ziwi promoter
Vector Backbone Description: Backbone Marker:Unknown; Backbone Size:9708; Vector Backbone:pTol2Dest-pGH_R4-R3; Vector Types:Cre/Lox, Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42403048
Comments: The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
Proper citation: RRID:Addgene_226785 Copy
Species: Danio rerio
Genetic Insert: ziwi promoter
Vector Backbone Description: Backbone Size:6946; Vector Backbone:pTol2Dest-pBH_R4-R2; Vector Types:Cre/Lox, Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42403048
Comments: The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
Proper citation: RRID:Addgene_226788 Copy
Species: Danio rerio
Genetic Insert: askopos promoter
Vector Backbone Description: Backbone Marker:Tol2kit v1.0; Backbone Size:2643; Vector Backbone:p5E-MCS; Vector Types:Gateway 5'-entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42403048
Comments: The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
Proper citation: RRID:Addgene_226791 Copy
Species: Danio rerio
Genetic Insert: Ace2N-mNeon
Vector Backbone Description: Vector Backbone:pDestTol pA2; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42109300
Comments: Please visit https://doi.org/10.1101/2024.12.12.628143 for bioRxiv preprint.
Proper citation: RRID:Addgene_253619 Copy
Species: Danio rerio
Genetic Insert: nanos2
Vector Backbone Description: Backbone Marker:Nathan Lawson; Backbone Size:2810; Vector Backbone:p5E-MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Cloned by Maya Pahima.
The putative zebrafish nos2 promoter was cloned from zebrafish ovary gDNA from a region directly upstream of the nos2 coding sequence with the following primers: 3’-ACTGGCCTAAAGGGTGTCCT-5’ and 3’-CGTCCCTTGCCTTTAGTCTG-5’. The cloned amplicon was inserted into the pCR8 cloning vector using the TOPO™ TA Cloning Kit (Thermo Fisher, K250020) for stabilization and sequencing. Putative nos2 promoter region was excised from the TOPO vector using EcoRI and SmaI, treated with T4 polymerase, then inserted into SmaI-linearized p5E-MCS (Addgene, #26029).
Proper citation: RRID:Addgene_226796 Copy
Species: Danio rerio
Genetic Insert: nanos2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2640; Vector Backbone:pDONR-P2R-P3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Cloned by Maya Pahima.
The putative zebrafish nos2 3'utr was cloned from zebrafish ovary gDNA using primers (3’-ATCAACCAATGCTGAATCTTTTTATAAAAAATTGAA-5' and 3’-AAATAAAATATATCAGGAGGAAACAAATATATCAGG-5') and inserted into the pCR4 cloning vector using the TOPO™ TA Cloning Kit (Thermo Fisher, K457502) for stabilization and sequencing (pCR4-nos2,3'utr). To insert the nos2,3’utr sequence into a Gateway Cloning vector, the region was amplified with attB primers (3'-GGGGACAGCTTTCTTGTACAAAGTGGTATATCAACCAATGCTGAATCTTTTTATAAAAAATTGAA-5' and 3'-GGGGACAACTTTGTATAATAAAGTTGTAAATAAAATATATCAGGAGGAAACAAATATATCAGG-5') and a BP reaction was performed with the Gateway BP Clonase II Enzyme mix (Invitrogen, 11789020) to recombine the amplicon into pDONR-P2R-P3 (Invitrogen, 12537-023) and produce p3E-nos2,3’utr.
Proper citation: RRID:Addgene_226799 Copy
Species: Danio rerio
Genetic Insert: zSynA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3961; Vector Backbone:PCRII-TOPO; Vector Types:in-situ; Bacterial Resistance:Ampicillin
Comments: zSynA in situ probe.
For antisense, cut with BamHI and transcribe with T7
Proper citation: RRID:Addgene_20121 Copy
Species: Danio rerio
Genetic Insert: birA tagRFP
Vector Backbone Description: Backbone Marker:Kawakami; Backbone Size:6173; Vector Backbone:pT2KXIGdeltaIn; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25231762
Comments: Note: Addgene's quality control sequencing has found some discrepancies with the depositor's uploaded sequence. The depositing lab is aware of these differences, and they are not thought to affect plasmid function.
Proper citation: RRID:Addgene_58378 Copy
Species: Danio rerio
Genetic Insert: birA EGFP
Vector Backbone Description: Backbone Marker:Kawakami; Backbone Size:6208; Vector Backbone:pT2KXIGdeltaIn; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25231762
Comments: Note: Addgene's quality control sequencing has found some discrepancies with the depositor's uploaded sequence. The depositing lab is aware of these differences, and they are not thought to affect plasmid function.
Proper citation: RRID:Addgene_58377 Copy
Species: Danio rerio
Genetic Insert: avi-EGFP-rpl10a
Vector Backbone Description: Backbone Marker:Kawakami; Backbone Size:6173; Vector Backbone:pT2KXIGdeltaIn; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25231762
Comments: Note: Addgene's quality control sequencing has found some discrepancies with the depositor's uploaded sequence. The depositing lab is aware of these differences, and they are not thought to affect plasmid function.
Proper citation: RRID:Addgene_58380 Copy
Species: Danio rerio
Genetic Insert: zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4777; Vector Backbone:pDONR P4-P1R; Vector Types:Gateway 5' entry clone; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_58890 Copy
Species: Danio rerio
Genetic Insert: elavl3 promoter
Vector Backbone Description: Vector Backbone:MiniTol2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25068735
Comments: Addgene NGS QC analysis identified multiple variants in the HuC promoter sequence.
Proper citation: RRID:Addgene_59531 Copy
Species: Danio rerio
Genetic Insert: elavl3 promoter
Vector Backbone Description: Vector Backbone:MiniTol2; Vector Types:Transposable element; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25068736
Comments: Addgene NGS QC analysis identified multiple variants in the HuC promoter sequence.
Proper citation: RRID:Addgene_59530 Copy
Species: Danio rerio
Genetic Insert: HSPB15
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18161059
Comments: linearize with NotI, transcribe with SP6, probe length is 556.
Proper citation: RRID:Addgene_61039 Copy
Species: Danio rerio
Genetic Insert: HSPB9
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18161059
Comments: linearize with NotI, transcribe with SP6, probe length is 556.
Proper citation: RRID:Addgene_61038 Copy
Species: Danio rerio
Genetic Insert: HSPB12
Vector Backbone Description: Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18161059
Comments: linearize with NotI, transcribe with SP6, probe length is 523.
Note: Addgene's quality control sequencing has found a few mismatches in the plasmid insert, but they should not affect its ability to be used as a probe.
Proper citation: RRID:Addgene_61037 Copy
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