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Species: Homo sapiens
Genetic Insert: TUBG1
Vector Backbone Description: Backbone Marker:AMRAD corparation limited; Backbone Size:4900; Vector Backbone:pGEX2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34158617
Comments: The TUBG1 gene from the plasmid obtained from Jiri Bartek was subcloned into pGEX2t. Addgene NGS found mutation R275H [NP_001061.2 ] but the depositor confirmed that this mutation does not affect function
Proper citation: RRID:Addgene_171967 Copy
Species: Homo sapiens
Genetic Insert: TUBG2
Vector Backbone Description: Backbone Marker:thermofisher; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34158617
Comments: The internal EcoRI was removed from the GeneCopeia mCherry tagged TUBG2/pReceiver-M56 plasmid and TUBG2 was sub-cloned into pcDNA3.1.
Proper citation: RRID:Addgene_171966 Copy
Species: Homo sapiens
Genetic Insert: F-box and leucine rich repeat protein 7
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32839549
Proper citation: RRID:Addgene_171848 Copy
Species: Homo sapiens
Genetic Insert: INPP5K
Vector Backbone Description: Vector Backbone:pCS Cherry DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33947811
Proper citation: RRID:Addgene_171950 Copy
Species: Homo sapiens
Genetic Insert: Chibby
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6600; Vector Backbone:pMAL-c2E; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The full coding sequence of human Chibby, including 3' UTR was amplified by PCR, digested with BglII and Xho1, and subcloned into the BamH1 and Sal1 sites of pMAL-c2E. The TEV protease site was included in the 5'primer. Insert size ~1kb.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_17195 Copy
Species: Homo sapiens
Genetic Insert: Human PE sequence (hPE)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3-Promoter; Vector Types:Mammalian Expression, Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33458694
Proper citation: RRID:Addgene_172003 Copy
Species: Homo sapiens
Genetic Insert: FKBP DD (L106P)-UbK0G75C
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33007216
Proper citation: RRID:Addgene_172001 Copy
Species: Homo sapiens
Genetic Insert: Rabaptin-5 (human)
Vector Backbone Description: Backbone Marker:Takara Biotech/Clontech; Backbone Size:4358; Vector Backbone:pBI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20169068
Proper citation: RRID:Addgene_171851 Copy
Species: Homo sapiens
Genetic Insert: c-SRC
Vector Backbone Description: Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32839549
Proper citation: RRID:Addgene_171850 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is an amino terminal HIS tag, TEV protease cleavage site, then human beta catenin domains R1C, subcloned in BamHI and Xho 1 sites.
Reference: Made by Ken-Ichi Takemaru.
Proper citation: RRID:Addgene_17200 Copy
Species: Homo sapiens
Genetic Insert: E3 ubiquitin-protein ligase RNF216
Vector Backbone Description: Backbone Size:5642; Vector Backbone:pOPINB; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34998453
Proper citation: RRID:Addgene_171922 Copy
Species: Homo sapiens
Genetic Insert: CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
Vector Backbone Description: Vector Backbone:pCMV-PE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Comments: Full sequence https://benchling.com/s/seq-LC7TExUM5DxOrjk2thkf.
Proper citation: RRID:Addgene_171996 Copy
Species: Homo sapiens
Genetic Insert: HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
Vector Backbone Description: Vector Backbone:pCMV-PE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Comments: Full sequence https://benchling.com/s/seq-s7KGcyKNgXZDawwnudbC.
Proper citation: RRID:Addgene_171995 Copy
Species: Homo sapiens
Genetic Insert: Tandem repeats Hybrid binding domain of Rnase H1
Vector Backbone Description: Vector Backbone:PGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33597247
Proper citation: RRID:Addgene_171870 Copy
Species: Homo sapiens
Genetic Insert: Endophilin A1
Vector Backbone Description: Vector Backbone:mRuby2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33947811
Proper citation: RRID:Addgene_171945 Copy
Species: Homo sapiens
Genetic Insert: AKTIP
Vector Backbone Description: Vector Backbone:mRuby2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33947811
Proper citation: RRID:Addgene_171942 Copy
Species: Homo sapiens
Genetic Insert: INPP5B
Vector Backbone Description: Vector Backbone:pCS Cherry DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33947811
Proper citation: RRID:Addgene_171947 Copy
Species: Homo sapiens
Genetic Insert: PIK3C2G
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33947811
Proper citation: RRID:Addgene_171946 Copy
Species: Homo sapiens
Genetic Insert: Chibby
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The N terminal half (amino acids 1-63) of human Chibby was amplified by PCR and subcloned into EcoR1 and Xho1 sites of pGEX-4T-1. A TEV protease site was included in the 5' primer.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_17196 Copy
Species: Homo sapiens
Genetic Insert: Chibby
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The C terminal half (amino acids 64-126) of human Chibby was amplified by PCR and subcloned into EcoR1 and Xho1 sites of pGEX-4T-1. A TEV protease site was included in the 5' primer. Insert contains 3'UTR of Cby.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_17197 Copy
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