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Species: Homo sapiens
Genetic Insert: HES1
Vector Backbone Description: Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16513761
Proper citation: RRID:Addgene_24982 Copy
Species: Homo sapiens
Genetic Insert: mir-9-3
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen, Addgene plasmid 11375; Backbone Size:6963; Vector Backbone:MDH1-PGK-GFP 2.0; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20173740
Comments: The mir-9-3 gene was PCR-amplified from normal human genomic DNA and cloned into the MDH-PGK-GFP 2.0 retroviral vector. It includes the miR-9 stem-loop and ~100bp flanking sequences on both sides.
Proper citation: RRID:Addgene_25036 Copy
Species: Homo sapiens
Genetic Insert: CDH1 mutant 3'UTR
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-report; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20173740
Comments: A partial CDH1 3'UTR (819bp) was PCR-amplified from normal human genomic DNA and cloned into the pMIR-report vector. The mutant construct of the CDH1 3′ UTR was generated with a QuikChange Site-Directed Mutagenesis Kit (Stratagene).
Proper citation: RRID:Addgene_25039 Copy
Species: Homo sapiens
Genetic Insert: STAT3
Vector Backbone Description: Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19074878
Comments: A V667L mutation is present but not known to affect function.
Expression of EGFP is driven by the human ubiquitin promoter. See Addgene plasmid 14883: FUGW for more information on the empty vector.
Proper citation: RRID:Addgene_24983 Copy
Species: Homo sapiens
Genetic Insert: CDH1 3'UTR
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-report; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20173740
Comments: A partial CDH1 3'UTR (819bp) was PCR-amplified from normal human genomic DNA and cloned into the pMIR-report vector.
Proper citation: RRID:Addgene_25038 Copy
Species: Homo sapiens
Genetic Insert: Rck
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4769; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19966221
Proper citation: RRID:Addgene_25033 Copy
Species: Homo sapiens
Genetic Insert: EDC3
Vector Backbone Description: Backbone Size:4769; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19966221
Proper citation: RRID:Addgene_25032 Copy
Species: C. briggsae
Genetic Insert: Cbr-unc-119
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2544; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20418868
Comments: [1-2] ENTR clone with cb-unc-119(+) insert.
Proper citation: RRID:Addgene_24980 Copy
Species: Homo sapiens
Genetic Insert: TNRC6A
Vector Backbone Description: Backbone Size:4770; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19383768
Proper citation: RRID:Addgene_25035 Copy
Species: Homo sapiens
Genetic Insert: DCP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4738; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19966221
Proper citation: RRID:Addgene_25031 Copy
Species: Homo sapiens
Genetic Insert: DCP1a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4769; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19966221
Proper citation: RRID:Addgene_25030 Copy
Species: C. stercorarium
Genetic Insert: xynB
Vector Backbone Description: Backbone Size:0; Vector Backbone:pPro; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20111002
Comments: DNA encoding the catalytic domain of xynB from C. stercorarium NCIMB 11754 was synthesized and codon-optimized by DNA 2.0. XynB was cloned using an EcoRI site on the 5' end and a BamHI site on the 3' end.
Proper citation: RRID:Addgene_24993 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7767; Vector Backbone:pcDNA-DEST53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16750377
Proper citation: RRID:Addgene_25048 Copy
Species: Homo sapiens
Genetic Insert: a mutant human MAP1-LC3B fused with HcRed
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pCXN2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18000393
Proper citation: RRID:Addgene_24992 Copy
Species: Mus musculus
Genetic Insert: Fgf10 promoter
Vector Backbone Description: Backbone Marker:ATTC; Backbone Size:6100; Vector Backbone:pXP1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12490567
Proper citation: RRID:Addgene_24994 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7767; Vector Backbone:pcDNA-DEST53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16750377
Proper citation: RRID:Addgene_25044 Copy
Species: Homo sapiens
Genetic Insert: a wild type human MAP1-LC3B fused with HcRed
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pCXN2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18000393
Proper citation: RRID:Addgene_24991 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7767; Vector Backbone:pcDNA-DEST53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16750377
Proper citation: RRID:Addgene_25046 Copy
Species: Homo sapiens
Genetic Insert: a mutant human MAP1-LC3B fused with EYFP
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pCXN2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18000393
Proper citation: RRID:Addgene_24990 Copy
Species: Xenopus laevis
Genetic Insert: insulin-like growth factor 1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11709186
Comments: mRNA injection sense: cut with NotI, transcribe with Sp6
Anti-sense in situ probe: cut with EcoRI, transcribe with T7
PCR fragment of IGF1 (EcoRI-XhoI) from stage 27 cDNA inserted into pCS2 (EcoRI-XhoI).
Sense: 5'AAA GAA TTC ATG GAA ACA AAC AAC AAT
Antisense: 5'-TTT CTC GAG TTA CAT TCG GAA TCC TCG
Proper citation: RRID:Addgene_24967 Copy
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