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Genetic Insert: Flp recombinase
Vector Backbone Description: Backbone Marker:Addgene Plasmid 11578; Backbone Size:6739; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20479239
Comments: pSico-Flpo allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression.
The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSico.html
Proper citation: RRID:Addgene_24969 Copy
Species: Xenopus laevis
Genetic Insert: Btg-X
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15879552
Comments: For sense mRNA cut with NotI, transcribe with SP6
For antisense cut with ClaI, transcribe with T7
Proper citation: RRID:Addgene_24964 Copy
Species: Xenopus laevis
Genetic Insert: labial
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1677215
Proper citation: RRID:Addgene_24965 Copy
Genetic Insert: pKW08-Lx-Int
Vector Backbone Description: Backbone Size:8535; Vector Backbone:pMC1M; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20434484
Proper citation: RRID:Addgene_25015 Copy
Species: Xenopus laevis
Genetic Insert: Antivin
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16172975
Comments: For antisense cut with HindIII, transcribe with T7
For sense cut with NotI, transcribe with SP6
Proper citation: RRID:Addgene_24961 Copy
Genetic Insert: pMEND-Lx
Vector Backbone Description: Backbone Size:8798; Vector Backbone:pMINDLx; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20434484
Proper citation: RRID:Addgene_25010 Copy
Genetic Insert: pMIND-Lx
Vector Backbone Description: Backbone Size:8798; Vector Backbone:pSHKLx; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20434484
Proper citation: RRID:Addgene_25013 Copy
Species: Xenopus laevis
Genetic Insert: Frzb-1 HA
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9118218
Comments: For sense, cut with NotI, transcribe with SP6.
HA tag is cloned between Ser158 and Met159 of Frezzled by PCR. HA tag includes a AatII diagnostic site.
Proper citation: RRID:Addgene_24979 Copy
Species: Xenopus laevis
Genetic Insert: Chordin
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS-SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8001117
Comments: Partial 2.8 kB clone of the original Xenopus chordin cDNA lacking 3' portion.
For antisense cut with EcoRI, transcribe with T7
For Northern blot probe, cut with EcoRI and XhoI
Proper citation: RRID:Addgene_24975 Copy
Species: Mus musculus
Genetic Insert: twisted gastrulation
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11714670
Comments: mRNA injection sense: cut with NotI, transcribe with SP6
Contains the Xenopus Chordin signal peptide and the N terminus until Ala41, followed by a Flag tag sequence.
Proper citation: RRID:Addgene_24974 Copy
Species: Drosophila melanogaster
Genetic Insert: HPat
Vector Backbone Description: Backbone Marker:Elisa Izaurralde; Backbone Size:6080; Vector Backbone:pAc5.1B-EGFP; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20404111
Proper citation: RRID:Addgene_25029 Copy
Genetic Insert: CMV-miR-31 sponge
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA5-CMV-d2eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19524507
Comments: miR-31 sponge generated by cloning oligonucleotides containing seven tandem “bulged” (at positions 9-12) miR-31 binding motifs (AGGCAAGACGAGGCATAGCT) into the XhoI and ApaI sites of the CMV sponge backbone.
Proper citation: RRID:Addgene_25025 Copy
Genetic Insert: synthetic miR-335 site
Vector Backbone Description: Backbone Marker:David Bartel, Addgene plasmid # 12179; Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19524507
Comments: Renilla luciferase reporter for miR-335 constructed by cloning oligo containing synthetic miR-335 binding site (GCCAGTGAGCTCTCAAGAGCAATAACGAAAAATGTACCGGTGCCAGT) into SacI and AgeI sites of the 3’ UTR of a pIS1 vector backbone.
Proper citation: RRID:Addgene_25028 Copy
Genetic Insert: synthetic miR-206 site
Vector Backbone Description: Backbone Marker:David Bartel, Addgene plasmid # 12179; Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19524507
Comments: Renilla luciferase reporter for miR-206 constructed by cloning oligo containing synthetic miR-206 binding site (GCCATTGAGCTCTGGAATGTAAGGAAGTGTGTGGACCGGTGCCATT) into SacI and AgeI sites of the 3’ UTR of a pIS1 vector backbone.
Proper citation: RRID:Addgene_25027 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7464; Vector Backbone:pEF-DEST51; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18397888
Proper citation: RRID:Addgene_25080 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7464; Vector Backbone:pEF-DEST51; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18397888
Proper citation: RRID:Addgene_25081 Copy
Species: Plasmodium vivax
Genetic Insert: Pv003920:C34523
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7737; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: PDB: 3B6N. http://www.thesgc.org/structures/3B6N/ .
Proper citation: RRID:Addgene_25118 Copy
Species: Cryptosporidium parvum
Genetic Insert: cgd5_3360:MAC029-C04:C34239
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7746; Vector Backbone:p15TV-L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: PDB: 3BE4. http://www.thesgc.org/structures/3BE4/ .
Proper citation: RRID:Addgene_25119 Copy
Species: Plasmodium falciparum
Genetic Insert: PFE1350c:C34884
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7737; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: PDB: 2R0J. h http://www.thesgc.org/structures/2R0J/ .
Proper citation: RRID:Addgene_25114 Copy
Species: Plasmodium falciparum
Genetic Insert: PF11_0301:C22904
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7746; Vector Backbone:p15TV-L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: PDB: 2PT9. http://www.thesgc.org/structures/2PT9/ .
Proper citation: RRID:Addgene_25110 Copy
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