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Species: Synthetic
Genetic Insert: sZF9
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503053
Proper citation: RRID:Addgene_43840 Copy
Species: Synthetic
Genetic Insert: sZF10
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503053
Proper citation: RRID:Addgene_43841 Copy
Species: Synthetic
Genetic Insert: sZF11
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503053
Proper citation: RRID:Addgene_43842 Copy
Species: Homo sapiens
Genetic Insert: DNAJA4 CDS
Vector Backbone Description: Backbone Size:5165; Vector Backbone:pBabe-PURO; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23142051
Proper citation: RRID:Addgene_43799 Copy
Species: Synthetic
Genetic Insert: sZF2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503053
Proper citation: RRID:Addgene_43833 Copy
Species: Synthetic
Genetic Insert: sZF4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503053
Proper citation: RRID:Addgene_43835 Copy
Species: Synthetic
Genetic Insert: sZF5
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503053
Proper citation: RRID:Addgene_43836 Copy
Species: Synthetic
Genetic Insert: sZF6
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503053
Proper citation: RRID:Addgene_43837 Copy
Species: Synthetic
Genetic Insert: sZF7
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503053
Proper citation: RRID:Addgene_43838 Copy
Species: Homo sapiens
Genetic Insert: LIN28B
Vector Backbone Description: Backbone Size:5372; Vector Backbone:pFRT/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23481595
Comments: Note that the Flag and HA tags are not in frame in the depositor's assembled sequence, but Addgene's quality control sequence shows they are in frame.
Proper citation: RRID:Addgene_43798 Copy
Vector Backbone Description: Backbone Marker:John Mekalanos lab, Harvard Medical School (PMID: 2836362); Backbone Size:5720; Vector Backbone:pGP704; Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB1; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9511756
Comments: The plasmids deposited here comprise a set of SacB1-dependent allelic exchange vectors improved from a previously described suicide vector, pGP704 (Miller and Mekalanos, 1988), by including a system to select for plasmid loss by recombination.
Plasmid pRE107 was constructed by cloning the appropriate EcoRI fragment from pUC58-sacB1 (McIver et al., 1995) into pGP704 (see associated schematic image). The sacB1 allele is a modified variation of sacB, where unique restriction sites were removed by site directed mutagenesis (McIver et al., 1995). Additionally, the BamHI site in the R6K ori of the resulting plasmid was removed by partial BamHI digestion, blunting the resulting overhangs with PolIk (resulting in the formation of a ClaI site) and screening for its loss by restriction analysis.
The resulting plasmid together with others in this deposited series contain the conditional R6K ori, the origin of transfer (oriT) which allows conjugative transfer from permissive hosts, the sacB1 gene to provide negative selection and a MCS, along with a range of different AbR markers.
Note: Addgene's NGS results found that a large portion of the plasmid backbone (containing the oriT feature) is in the opposite orientation compared to the depositor's reference sequence.
Proper citation: RRID:Addgene_43829 Copy
Species: Homo sapiens
Genetic Insert: WNT10B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43823 Copy
Species: Homo sapiens
Genetic Insert: human WNT11
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43824 Copy
Species: Homo sapiens
Genetic Insert: WNT8A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43818 Copy
Species: Homo sapiens
Genetic Insert: WNT8B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43819 Copy
Species: Homo sapiens
Genetic Insert: WNT4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43812 Copy
Species: Homo sapiens
Genetic Insert: WNT5A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43813 Copy
Species: Homo sapiens
Genetic Insert: WNT5B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43814 Copy
Species: Homo sapiens
Genetic Insert: human WNT7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43816 Copy
Genetic Insert: TALE-activator p65 backbone
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396285
Comments: Location of plasmid features:
EF1a = 922-2106
start codon = 2119-2121
NLS = 2122-2165
N-term = 2166-2577
0.5 domain = 2592-2658
C-term = 2665-2949
p65 = 2959-3768
pA = 3775-3894
Proper citation: RRID:Addgene_43891 Copy
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