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Genetic Insert: SARS-CoV-2-RBD-B.1.621
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.12.29.474491v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181700 Copy
Genetic Insert: SARS-CoV-2-S2P-B.1.621
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.12.29.474491v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181694 Copy
Species: Rattus norvegicus
Genetic Insert: lysosome associated membrane protein 1
Vector Backbone Description: Backbone Marker:BD Biosciences / Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14617360
Comments: Lamp-1 sequence contains a D50E mutation, which is not important for function of plasmid. There is also a silent mutation at bp#715 compared to GenBank NM_012857.1
Proper citation: RRID:Addgene_1816 Copy
Species: Rattus norvegicus
Genetic Insert: lysosome associated membrane protein 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Modified Clontech Plasmid; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14617360
Comments: Lamp-1 sequence contains a D50E mutation, which is not important for function of plasmid. There is also a silent mutation at bp#715 compared to GenBank NM_012857.1
Proper citation: RRID:Addgene_1817 Copy
Genetic Insert: SARS-CoV-2-RBD-C.37
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.12.29.474491v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181699 Copy
Genetic Insert: SARS-CoV-2-S2P-B.1.1.529
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.12.29.474491v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181695 Copy
Genetic Insert: SARS-CoV-2-NTD-B.1.1.529
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.12.29.474491v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181698 Copy
Species: Homo sapiens
Genetic Insert: BTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32437509
Proper citation: RRID:Addgene_180806 Copy
Species: Mus musculus
Genetic Insert: PHR-mCherry-nE-mHP1alpha-delCTE
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3958; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Comments: For additional reference on HP1alpha, please see Larson et al. 2017 Nature, PMID 28636604, https://doi.org/10.1038/nature22822
Proper citation: RRID:Addgene_181891 Copy
Species: Mus musculus
Genetic Insert: RGG-GFP-RGG
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Comments: PCR for RGG-GFP-RGG on Addgene plasmid (#124939),cloned into GFP-mHP1a using AgeI (BshTI) & BspEI (Kpn2I) --> cut out GFP, insert RGG-GFP-RGG
Proper citation: RRID:Addgene_181898 Copy
Species: Homo sapiens
Genetic Insert: FUSN-mHP1alpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Comments: PCR for FUSN on Addgene plasmid (#122148)cloned into GFP-mHP1a using BsrGI (Bsp1407I) & BspEI (Kpn2I)
Proper citation: RRID:Addgene_181897 Copy
Species: Mus musculus
Genetic Insert: PHR-mCherry-PGL-1-mHP1alpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3958; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Proper citation: RRID:Addgene_181894 Copy
Genetic Insert: SARS-CoV-2-RBD-SD1-C.37
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.12.29.474491v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181702 Copy
Species: Mus musculus
Genetic Insert: mHP1alpha
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Proper citation: RRID:Addgene_181911 Copy
Species: Mus musculus
Genetic Insert: mHP1alpha
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Proper citation: RRID:Addgene_181912 Copy
Species: Homo sapiens
Genetic Insert: PHR-mCherry-hNPM
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3958; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Comments: cloned hNPM into pPHR-mCherry Vector via XbaI/SalI
Proper citation: RRID:Addgene_181887 Copy
Species: Mus musculus
Genetic Insert: PHR-mCherry-mHP1alpha-I163A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3958; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Proper citation: RRID:Addgene_181889 Copy
Species: Mus musculus
Genetic Insert: PHR-mCherry-MECP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3958; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Proper citation: RRID:Addgene_181888 Copy
Species: Mus musculus
Genetic Insert: PHR-mCherry-mHP1alpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3958; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Comments: cloned mouse HP1a into PHR-mCherry vector and introduced another cloning site between mCherry and mHP1a to quickly insert droplet forming proteins (e. g. FUSN, PGL-1, RGG...)
Proper citation: RRID:Addgene_181883 Copy
Species: Synthetic
Genetic Insert: attB-EGFP-EF1α-PuroR-T2A-TagBFP
Vector Backbone Description: Vector Backbone:pEF1α; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34887556
Proper citation: RRID:Addgene_181923 Copy
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