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Species: Mus musculus
Genetic Insert: Foxo1 6KR
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16154098
Comments: Plasmid has K219R and L619P polymorphisms which are not thought to affect plasmid function.
Proper citation: RRID:Addgene_17560 Copy
Species: Drosophila melanogaster
Genetic Insert: mini w+
Vector Backbone Description: Backbone Size:2482; Vector Backbone:na; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18621688
Comments: pBDP is a modular minimal cloning vector for specific in vivo genomic
targeting of Drosophila melanogaster using PhiC31 integrase. The vector
contains mini w+ flanked by AscI sites for easy exchange to any marker of
choice as well as a large MCS, and the pMB1 ori from pUC19.
Proper citation: RRID:Addgene_17566 Copy
Species: Mus musculus
Genetic Insert: TLX2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9550720
Comments: See author's map.
Proper citation: RRID:Addgene_17567 Copy
Species: Homo sapiens
Genetic Insert: OPN-a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pDest-490; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17452979
Comments: Mature human OPN cDNA.
Proper citation: RRID:Addgene_17590 Copy
Species: Drosophila melanogaster
Genetic Insert: CG4845
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3500; Vector Backbone:pMT/V5-His B; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_17589 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728840
Comments: Please note that Addgene's sequencing results identified a V5 deletion in the ubiquitin sequence. This deletion was not intentional, but was present in the original source clone from which this plasmid was created (see associated publication for details).
Proper citation: RRID:Addgene_17602 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728840
Proper citation: RRID:Addgene_17604 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728840
Proper citation: RRID:Addgene_17605 Copy
Species: Homo sapiens
Genetic Insert: X-ray repair cross complementing 1
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34731617
Proper citation: RRID:Addgene_176062 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728840
Proper citation: RRID:Addgene_17606 Copy
Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:9377; Vector Backbone:pLX304; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27416908
Proper citation: RRID:Addgene_175846 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176016 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176015 Copy
Species: Homo sapiens
Genetic Insert: IFN gamma promoter with 1st intron enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10640755
Comments: Young lab stock #D3314.
SalI should give 8.6kb + 0.6kb bands.
Proper citation: RRID:Addgene_17598 Copy
Species: Homo sapiens
Genetic Insert: IFN gamma promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10640755
Comments: Young lab stock #D3299.
SalI should give 8.5kb band
Proper citation: RRID:Addgene_17599 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11078524
Proper citation: RRID:Addgene_17612 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11078524
Proper citation: RRID:Addgene_17613 Copy
Species: Homo sapiens
Genetic Insert: EF-1a promoter
Vector Backbone Description: Backbone Marker:Dr. Didier Trono; Backbone Size:0; Vector Backbone:SIN LV pRLLhPGK.GFP Sin-18 (modified); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11781219
Comments: SIN lentiviral vector that expresses GFP controlled by the promoter of the housekeeping gene EF-1alpha.
We constructed EF.GFP by replacing the human PGK promoter (EcoRV to BamHI sites in the PGK.GFP vector) with human EF-1alpha promoter from plasmid pEF1/Myc-His-A (Invitrogen, Carlsbad, CA). The EF-1alpha promoter (nucleotide 373 to 1560 as numbered in GenBank accession J04616) contains the first exon, first intron, and part of the second exon before the coding sequence (starting at nucleotide 1582).
Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells.
Proper citation: RRID:Addgene_17616 Copy
Species: Drosophila melanogaster
Genetic Insert: L596_g9608.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893
Proper citation: RRID:Addgene_176078 Copy
Species: Drosophila melanogaster
Genetic Insert: L596_g25050.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893
Proper citation: RRID:Addgene_176079 Copy
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