Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728840
Comments: The wild-type ubiquitin sequence in this plasmid is: MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG
Proper citation: RRID:Addgene_17608 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc/his A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16269541
Comments: Compared to GenBank reference sequence NP_940980.3, the LRRK2 insert in this plasmid contains a M2397T mutation.
Proper citation: RRID:Addgene_17609 Copy
Species: Mus musculus
Genetic Insert: rhotekin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37449837
Comments: Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint.
Proper citation: RRID:Addgene_176108 Copy
Species: Homo sapiens
Genetic Insert: Paxillin
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34084168
Proper citation: RRID:Addgene_176107 Copy
Species: Mus musculus
Genetic Insert: rhotekin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34357388
Proper citation: RRID:Addgene_176101 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728840
Comments: Please note that Addgene's sequencing results identified P19S and E24D mutations in the ubiquitin sequence. These mutations were not intentional, but were present in the original source clone from which this plasmid was created (see associated publication for details).
Proper citation: RRID:Addgene_17607 Copy
Species: Mus musculus
Genetic Insert: rhotekin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34357388
Proper citation: RRID:Addgene_176091 Copy
Species: Homo sapiens
Genetic Insert: RAC1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34357388
Proper citation: RRID:Addgene_176095 Copy
Species: Mus musculus
Genetic Insert: rhotekin
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34357388
Proper citation: RRID:Addgene_176098 Copy
Species: Homo sapiens
Genetic Insert: human Rb
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBSK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_1761 Copy
Species: Synthetic
Genetic Insert: iLID
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37449837
Comments: Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint.
Proper citation: RRID:Addgene_176125 Copy
Species: Drosophila melanogaster
Genetic Insert: L596_g9608.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893
Proper citation: RRID:Addgene_176082 Copy
Species: Aequorea victoria
Genetic Insert: NLS-2xBFP(4C)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4404; Vector Backbone:pTrcHisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35013558
Proper citation: RRID:Addgene_176151 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34731617
Proper citation: RRID:Addgene_176150 Copy
Vector Backbone Description: Backbone Size:8220; Vector Backbone:EF.CMV.RFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12788657
Comments: In a SIN LV containing the elongation factor 1alpha promoter, we included a second promoter from cytomegalovirus that drives expression of RFP as a reporter. Dual-promoter LVs can coexpress multiple transgenes efficiently in a single target cell.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
There is an a->g mutation in this plasmid that abolishes the HpaI site. A 10bp insertion in Addgene sequence corresponding to position 6893, 21bp insertion in Addgene sequence at position 7405, and 11bp deletion in Addgene sequence at position 7544 do not alter function of the plasmid or RFP expression.
The uploaded author's map shows an example with GFP cloned downstream of the EF-1a promoter. The vector supplied by Addgene does not contain the GFP.
Proper citation: RRID:Addgene_17619 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:5050; Vector Backbone:pAAV-CBA-w; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32184391
Proper citation: RRID:Addgene_176215 Copy
Species: Homo sapiens
Genetic Insert: HLA-DRa promoter
Vector Backbone Description: Backbone Marker:Dr. Didier Trono; Backbone Size:0; Vector Backbone:SIN LV pRLLhPGK.GFP Sin-18 (modified); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11781219
Comments: SIN lentiviral vector that expresses GFP controlled by the promoter of the human HLA-DRalpha gene, which is selectively expressed in antigen-presenting cells (APCs).
We constructed DR.GFP by using the human (MHC II) HLA-DRalpha promoter (nucleotide 182 to 480, as numbered in GenBank accession X00274) to replace the PGK promoter (XhoI to BamHI sites in the PGK.GFP vector). This 300-base pair promoter sequence has been shown to confer selective expression of a transgene in MHC II+ cells.
Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells.
Proper citation: RRID:Addgene_17617 Copy
Species: Mus musculus
Genetic Insert: delFasL
Vector Backbone Description: Backbone Size:0; Vector Backbone:EF.CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12788657
Comments: The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP, with a unique EcoRV site available to insert a gene of interest under the control of the EF promoter. Noncleavable mouse delFasL cDNA was constructed using PCR cloning to delete the exon 2 region, which contains the metalloproteinase cleavage site. The delFasL gene was then cloned into the EcoRV site of EF.CMV.GFP to make the EF.delFasL-CMV.GFP LV.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17620 Copy
Species: Homo sapiens
Genetic Insert: ICN1
Vector Backbone Description: Backbone Marker:Linzhao Cheng Lab; Backbone Size:8200; Vector Backbone:EF.v-CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16513761
Comments: Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function.
The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. hICN1 was cloned downstream of the EF-1a promoter.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17623 Copy
Species: Homo sapiens
Genetic Insert: ICN1
Vector Backbone Description: Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16513761
Comments: Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function.
hICN1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17626 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within RRID that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
