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Species: Homo sapiens
Genetic Insert: WNT2B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43808 Copy
Genetic Insert: TALE-activator p65 backbone
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396285
Comments: Location of plasmid features:
EF1a = 922-2106
start codon = 2119-2121
NLS = 2122-2165
N-term = 2166-2577
0.5 domain = 2592-2658
C-term = 2665-2949
p65 = 2959-3768
pA = 3775-3894
Proper citation: RRID:Addgene_43889 Copy
Species: Homo sapiens
Genetic Insert: Human Optimized S. pyogenes Cas9
Vector Backbone Description: Backbone Size:6670; Vector Backbone:p415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23460208
Proper citation: RRID:Addgene_43804 Copy
Species: Mus musculus
Genetic Insert: Hes1 Promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7906273
Proper citation: RRID:Addgene_43806 Copy
Genetic Insert: SID4X Domain
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22828628
Comments: This enhanced SID domain (SID4X domain) is to be used with TALE effectors to create TALE repressors, as described in Cong et al. Nature Communications. This new domain replaces the previous SID domain reported in the original article.
Proper citation: RRID:Addgene_43882 Copy
Genetic Insert: TALE-activator VP64 Backbone
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396285
Comments: Location of plasmid features:
EF1a = 922-2106
start codon - 2119-2121
NLS = 2122-2165
N-term =2166-2577
0.5 domain = 2592-2658
C-term = 2665-2949
VP64 = 2956-3122
pA = 3125-3244
Proper citation: RRID:Addgene_43885 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DIF1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18851834
Comments: The following mutations were found during the Addgene QC sequencing: Q31 deletion, S60G and Q68H.
Proper citation: RRID:Addgene_43909 Copy
Species: Homo sapiens
Genetic Insert: USP44
Vector Backbone Description: Backbone Size:4700; Vector Backbone:p142; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17443180
Proper citation: RRID:Addgene_43903 Copy
Species: Homo sapiens
Genetic Insert: USP44
Vector Backbone Description: Backbone Size:9692; Vector Backbone:p332; Vector Types:Insect Expression, baculoviral expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17443180
Proper citation: RRID:Addgene_43905 Copy
Species: Homo sapiens
Genetic Insert: TNF receptor-associated factor 3
Vector Backbone Description: Backbone Marker:Shao-Cong Sun; Vector Backbone:pcDNA-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15084608
Comments: pCDNA-HA backbone: Inserted influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) with the translation initiation codon (ATG) into pcDNA3.1 vector (Invitrogen); described in Harhaj, et al. (1999) JBC, 274, 22911–22914.
Please note that the hTRAF3 insert in this plasmid contains V274D and S393G mutations when compared to GenBank entry NP_663777.1
Proper citation: RRID:Addgene_44034 Copy
Species: Homo sapiens
Genetic Insert: TNF receptor-associated factor 3
Vector Backbone Description: Backbone Marker:Shao-Cong Sun; Vector Backbone:pcDNA-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15084608
Comments: pCDNA-HA backbone: Inserted influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) with the translation initiation codon (ATG) into pcDNA3.1 vector (Invitrogen); described in Harhaj, et al. (1999) JBC, 274, 22911–22914.
Please note that the hTRAF3 insert in this plasmid contains V274D and S393G mutations when compared to GenBank entry NP_663777.1
Proper citation: RRID:Addgene_44033 Copy
Genetic Insert: AGA1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9181578
Proper citation: RRID:Addgene_44036 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-Tax-3'UTR
Vector Backbone Description: Backbone Marker:Dr. Inder Verma; Vector Backbone:pCLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11553609
Comments: Transfer of BamHI-BamHI fragment covering 5'UTR-Tax-3'UTR from pCMV4-Tax (from Dr. Warner Greene) to the BamHI site of pCLXSN (from Dr. Verma Inder).
Proper citation: RRID:Addgene_44038 Copy
Species: Mus musculus
Genetic Insert: mHIF-2α (P405A/P530V/N851A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17804822
Comments: This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-2α.
Full-length murine HIF-2α cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites.
A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-2α cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 405, Proline 530 and Asparagine 851. The newly constructed plasmid was sequenced to confirm encoding of the correct protein.
This plasmid has an A94V polymorphism that does not affect protein function.
Alternate plasmid names: HIF-2α triple mutant (TM); pcDNA3 HIF-2α TM
Proper citation: RRID:Addgene_44027 Copy
Vector Backbone Description: Backbone Size:5500; Vector Backbone:p416-GPD; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21586584
Proper citation: RRID:Addgene_43972 Copy
Species: Mus musculus
Genetic Insert: mHIF-1α (P402A/P577A/N813A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17804822
Comments: This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-1α.
Full-length murine HIF-1α cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites.
A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-1α cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 402, Proline 577 and Asparagine 813 to alanines. The newly constructed plasmid was sequenced to confirm encoding of the correct protein.
Alternate plasmid names: HIF-1α triple mutant (TM); pcDNA3 HIF-1α TM
Proper citation: RRID:Addgene_44028 Copy
Species: Synthetic
Genetic Insert: HRP
Vector Backbone Description: Backbone Size:6142; Vector Backbone:pCTcon2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17961829
Proper citation: RRID:Addgene_44021 Copy
Species: Mus musculus
Genetic Insert: Cbx5
Vector Backbone Description: Backbone Size:8600; Vector Backbone:N103; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704655
Proper citation: RRID:Addgene_43969 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal4/DBD (1-147)
Vector Backbone Description: Backbone Size:8600; Vector Backbone:N103; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704655
Proper citation: RRID:Addgene_44014 Copy
Species: Homo sapiens
Genetic Insert: FKBP
Vector Backbone Description: Backbone Size:8600; Vector Backbone:N103; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704655
Proper citation: RRID:Addgene_44017 Copy
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