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Species: Saccharomyces cerevisiae
Genetic Insert: ADE2
Vector Backbone Description: Vector Backbone:pJJ215; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid was made by cutting pJJ215 with MscI and Nsi I and replacing the 570 bp MscI–Nsi I fragment with a 3.6 kb NotI (blunted with Klenow)–PstI fragment containing ADE2 from plasmid M1144. Plasmid M1144 contains a 3.6 kb BglII fragment (partial digest product) with ADE2 with BamHI linkers cloned into the BamHI site of Bluescript KS+.
Proper citation: RRID:Addgene_51671 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2
Vector Backbone Description: Vector Backbone:pJJ244; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid M3499 (ura3::ADE2 ) was made by cutting pJJ244 with StuI and EcoRV and replacing the 248 bp StuI–EcoRV fragment with a 3.6 kb BamHI blunted with Klenow) fragment containing ADE2 from plasmid M1144.
Proper citation: RRID:Addgene_51674 Copy
Genetic Insert: TAL21
Vector Backbone Description: Vector Backbone:pZDONOR 5-6; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25369267
Proper citation: RRID:Addgene_51795 Copy
Species: Synthetic
Genetic Insert: EBFP2
Vector Backbone Description: Vector Backbone:pZDONOR 5-6; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25369267
Proper citation: RRID:Addgene_51790 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LYS2
Vector Backbone Description: Vector Backbone:YDp-H; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid was made by cutting YDp-H with BglII and replacing the 60 bp BglII fragment with a 5 kb BamHI fragment (partial digest product) containing LYS2 from plasmid YDp-K.
Proper citation: RRID:Addgene_51676 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LYS2
Vector Backbone Description: Backbone Marker:Marsh et al., 1984; Vector Backbone:M581; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid M2660 (ura3::LYS2 ) was made by cutting M581 with EcoRV and PstI and replacing the 208 bp EcoRV–PstI fragment with a 4.6 kb PvuII–PstI fragment containing LYS2 from plasmid YDp-K.
Proper citation: RRID:Addgene_51678 Copy
Vector Backbone Description: Vector Backbone:pBluescript KS+; Vector Types:T. brucei genome integration vectors; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16278461
Comments: The PTP tag is a derivative of the original TAP tag and has CBP replaced by ProtC.
Gene flanks providing RNA processing signals for PTP fusion and resistance marker are the 3′ flank of TbRPA1 (RPA1-3′), HSP70 genes 2 and 3 intergenic region (H23), the β-α tubulin intergenic region (T), and the 5′ flank of TbRPA2 (RPA2-5′).
pC-PTP-NEO contains a target sequence (U1-70K) which can be replaced by the gene of interest.
Proper citation: RRID:Addgene_51710 Copy
Species: Homo sapiens
Genetic Insert: MOV10 K530A
Vector Backbone Description: Backbone Size:5299; Vector Backbone:pFRT/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24726324
Proper citation: RRID:Addgene_51716 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: M4756 (kanMX::LEU2)was made by digesting M4757 with BamHI and SphI and inserting a 2.0 kb BamHI–SphI fragment with LEU2 from plasmid pJJ250.
Proper citation: RRID:Addgene_51685 Copy
Genetic Insert: KanMX3
Vector Backbone Description: Vector Backbone:YDp-L; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid M3926 (leu2::KanMX3 ) was made by cutting YDp-L with EcoRV and inserting a 2.5 kb BglII–EcoRI (both ends blunted with Klenow)fragment containing KanMX3 from plasmid pFA6–KanMX3.
Proper citation: RRID:Addgene_51680 Copy
Species: Homo sapiens
Genetic Insert: MOV10 D645N
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5882; Vector Backbone:pDEST26; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24726324
Proper citation: RRID:Addgene_51720 Copy
Species: Synthetic
Genetic Insert: aDTX-lumitoxin
Vector Backbone Description: Backbone Size:5341; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24407101
Proper citation: RRID:Addgene_51686 Copy
Species: Synthetic
Genetic Insert: SpyLigase
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:6400; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24639550
Comments: SnoopLigase is the more recent peptide-peptide ligation technology from the Howarth lab and is now preferred over SpyLigase in terms of more general reaction conditions and higher coupling yield on a range of proteins.
pET28a-AviTag-SnoopLigase https://www.addgene.org/105626/
pET28a-HaloTag7-SnoopLigase https://www.addgene.org/105627/
SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Jacob O. Fierer, Gianluca Veggiani and Mark Howarth
Transform plasmid into BL21 DE3 RIPL for expression.
Proper citation: RRID:Addgene_51722 Copy
Species: Mus musculus
Genetic Insert: 18S
Vector Backbone Description: Backbone Marker:promega; Backbone Size:2000; Vector Backbone:pCMV; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22718970
Comments: Please note that the depositor sequence is theoretical. Addgene verified that the nucleotides A5152 (nucleotide 963 in 18 S rRNA) and G5162 are expected in 18S, based on the publication and GenBank ID: JQ247698.1. Please see Addgene's result using the m18Sinternal-rev primer in the "Sequences" link above.
Proper citation: RRID:Addgene_51728 Copy
Species: Synthetic
Genetic Insert: Optopatch2
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9236; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24952910
Proper citation: RRID:Addgene_51694 Copy
Species: Synthetic
Genetic Insert: Optopatch1
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9236; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24952910
Proper citation: RRID:Addgene_51695 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Set2
Vector Backbone Description: Backbone Marker:commercial; Backbone Size:5400; Vector Backbone:pcDNA3.1 +; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20133523
Proper citation: RRID:Addgene_51698 Copy
Genetic Insert: EMCV IRES
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4600; Vector Backbone:pGl4.13; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22718970
Proper citation: RRID:Addgene_51733 Copy
Genetic Insert: MCS
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4600; Vector Backbone:pGl4.13; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22718970
Proper citation: RRID:Addgene_51732 Copy
Species: Homo sapiens
Genetic Insert: SirT1
Vector Backbone Description: Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19390637
Proper citation: RRID:Addgene_51746 Copy
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