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Genetic Insert: Mxi1+SV40 NLS
Vector Backbone Description: Vector Backbone:MoClo-YTK; Vector Types:Synthetic Biology, Moclo vector level 0 (type 4a); Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32884066
Proper citation: RRID:Addgene_163715 Copy
Genetic Insert: sgRNA expression cassette
Vector Backbone Description: Vector Backbone:MoClo-YTK; Vector Types:Synthetic Biology, Moclo vector level 0 (type 234); Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32884066
Proper citation: RRID:Addgene_163717 Copy
Genetic Insert: VPR
Vector Backbone Description: Vector Backbone:MoClo-YTK; Vector Types:Synthetic Biology, Moclo vector level 0 (type 4a); Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32884066
Proper citation: RRID:Addgene_163716 Copy
Species: Synthetic
Genetic Insert: hhrz-supP
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33290521
Proper citation: RRID:Addgene_163732 Copy
Species: Other
Genetic Insert: Marchantia polymorpha petB 5'UTR
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34097383
Proper citation: RRID:Addgene_163933 Copy
Species: Other
Genetic Insert: Nicotiana tabacum psba terminator
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32163700
Proper citation: RRID:Addgene_163932 Copy
Species: Other
Genetic Insert: aadA gene for spectinomycin resistance
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32163700
Proper citation: RRID:Addgene_163935 Copy
Species: Other
Genetic Insert: Marchantia polymorpha chloroplast DNA-dependent RNA polymerase operon promoter (rpo) (genome position: 5595-5739)
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32163700
Proper citation: RRID:Addgene_163951 Copy
Species: Other
Genetic Insert: Nicotiana tabacum psbA promoter
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34097383
Proper citation: RRID:Addgene_163950 Copy
Vector Backbone Description: Backbone Size:5352; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33271061
Comments: Reference for vector backbone.
Guzman, L. M., Belin, D., Carson, M. J., & Beckwith, J. (1995). Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. Journal of Bacteriology, 177(14), 4121–4130.
Proper citation: RRID:Addgene_164263 Copy
Species: Bergeyella zoohelcum
Genetic Insert: BzCas13b-gRNA-3
Vector Backbone Description: Backbone Size:4215; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34694607
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164860 Copy
Species: Leptotrichia buccalis
Genetic Insert: AcrVIA7
Vector Backbone Description: Backbone Size:4010; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34694607
Comments: The anti-CRISPR protein AcrVIA7 was codon-optimized for E. coli for optimal expression.
Proper citation: RRID:Addgene_164864 Copy
Species: Bergeyella zoohelcum
Genetic Insert: BzCas13b-gRNA-1
Vector Backbone Description: Backbone Size:4215; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34694607
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164858 Copy
Species: Yellow fever virus
Genetic Insert: YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry
Vector Backbone Description: Backbone Size:8888; Vector Backbone:pCCI; Vector Types:transneuronal tracing; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34824475
Comments: Please view the attached protocol for growing plasmid in the lab. If protocol is not followed it is likely DNA preps will contain a significant number of DNA impurities.
Proper citation: RRID:Addgene_179951 Copy
Species: Yellow fever virus
Genetic Insert: YFV-17D lacking C, M, E and NS1 proteins but with Cre
Vector Backbone Description: Backbone Size:8888; Vector Backbone:pCCI; Vector Types:transneuronal tracing; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34824475
Comments: Please view the attached protocol for growing plasmid in the lab. If protocol is not followed it is likely DNA preps will contain a significant number of DNA impurities.
Proper citation: RRID:Addgene_179954 Copy
Vector Backbone Description: Vector Backbone:pSEVA331; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34767345
Comments: Bpi I restriction site removed from replication protein coding gene. MCS replaced with Consitutive GFP drop-out TU with KTK GG overhands for D1.1 cloning. Read the preprint at https://www.biorxiv.org/content/10.1101/2021.06.09.447691v1.full
Proper citation: RRID:Addgene_180520 Copy
Vector Backbone Description: Vector Backbone:pSEVA331; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34767345
Comments: Bpi I restriction site removed from replication protein coding gene. MCS replaced with Consitutive GFP drop out TU with KTK GG overhands for D1.2 cloning. Read the preprint at https://www.biorxiv.org/content/10.1101/2021.06.09.447691v1.full
Proper citation: RRID:Addgene_180521 Copy
Vector Backbone Description: Vector Backbone:pSEVA331; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34767345
Comments: Bpi I restriction site removed from replication protein coding gene. MCS replaced with Consitutive LacZ drop out TU with KTK GG overhands for D1.2 cloning. Read the preprint at https://www.biorxiv.org/content/10.1101/2021.06.09.447691v1.full
Proper citation: RRID:Addgene_180519 Copy
Species: Synthetic
Genetic Insert: mNeonGreen; tetR
Vector Backbone Description: Backbone Size:1706; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35994658
Proper citation: RRID:Addgene_181944 Copy
Species: Synthetic
Genetic Insert: human/zebrafish codon optimized SpCas9
Vector Backbone Description: Vector Backbone:BPK764 (Addgene Plasmid #65767); Vector Types:Bacterial Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36203014
Proper citation: RRID:Addgene_181745 Copy
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