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Species: Synthetic
Genetic Insert: sgRNA expression cassette
Vector Backbone Description: Backbone Size:9200; Vector Backbone:pWH1266; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:39162403
Comments: Gebhardt lab ID # pMJG440. 5' Cloning Site: SpeI (Not Destroyed), 3' Cloning Site: ApaI (Not Destroyed).
Proper citation: RRID:Addgene_218372 Copy
Vector Backbone Description: Vector Backbone:p15A and pWH1266; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39302127
Comments: Shuttle vector that replicates in A. baumannii and E. coli; replicates in common cloning strains such as DH10B.
Proper citation: RRID:Addgene_222350 Copy
Species: Derived from Aequorea victoria.
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:p15A and pWH1266; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39302127
Comments: Shuttle vector that replicates in A. baumannii and E. coli; replicates in common cloning strains such as DH10B.
Proper citation: RRID:Addgene_222352 Copy
Species: Derived from Aequorea victoria.
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:R6Kγ; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39302127
Comments: Tn7 expression vector.
Proper citation: RRID:Addgene_222355 Copy
Species: Francisella novicida U112
Genetic Insert: FnCpf1
Vector Backbone Description: Vector Backbone:pMMB67EH; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:38248339
Proper citation: RRID:Addgene_214198 Copy
Vector Backbone Description: Vector Backbone:pMMB207; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39162403
Comments: Gebhardt lab ID # pMJG584.
Proper citation: RRID:Addgene_218358 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: scbe3-NG-Hypa
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218161 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: escbe3-NG-HF1
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218164 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: escbe3-NG-Hypa
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218165 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: scbe3-NG-HF1-Hypa
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218162 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: scbe3-Hypa
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218157 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: scbe3-HF1-Hypa
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218158 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: scbe3-NG
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218159 Copy
Species: S. coelicolor (bacteria)
Genetic Insert: escbe3-NG-HF1-Hypa
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin
Defining Citation: PMID:38971862
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218166 Copy
Species: Stackebrandtia nassauensis
Genetic Insert: inactivated SnaO
Vector Backbone Description: Vector Backbone:pOSV801; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:38947204
Comments: also encodes SnaA, SnaB, SnaC, SnaD, SnaE, SnaT1, SnaT2
Proper citation: RRID:Addgene_225058 Copy
Species: Streptomyces albidoflavus
Genetic Insert: SaindC
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pSET152; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:34489377
Proper citation: RRID:Addgene_177204 Copy
Genetic Insert: nCas9-BEC-UGI; cas6f, gfp
Vector Backbone Description: Backbone Marker:Quanjiang Ji (Addgene plasmid # 113349); Backbone Size:5000; Vector Backbone:pnCas9PA-BEC; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:35641501
Proper citation: RRID:Addgene_187599 Copy
Vector Backbone Description: Vector Backbone:pIJ8668; Vector Types:Bacterial Expression, Streptomyces expression; Bacterial Resistance:Apramycin
Comments: GenBank reference sequence JN636796 for NitR
Proper citation: RRID:Addgene_78577 Copy
Species: Synthetic
Genetic Insert: codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:40138716
Comments: Please visit https://doi.org/10.1101/2023.05.09.539971 for bioRxiv preprint.
The used type I-C CRISPR system was first described by:
Csörgő, B., León, L.M., Chau-Ly, I.J. et al. A compact Cascade–Cas3 system for targeted genome engineering. Nat Methods 17, 1183–1190 (2020). https://doi.org/10.1038/s41592-020-00980-w
Plasmid contains an IS prokaryotic transposable element that does not affect plasmid function.
The sequences used in pCRISPR-Cas3 were obtained via gene synthesis following codon optimization for S. coelicolor.
Proper citation: RRID:Addgene_209427 Copy
Species: Synthetic
Genetic Insert: Codon optimized APOBEC1-nCas9-UGI fusion protein
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:38340355
Proper citation: RRID:Addgene_209448 Copy
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